Bio-protocol - Improve Research Reproducibility

关键词: BS-seq

Bio-protocol
Bio-101

研究领域

生物化学
生信
生物信息学与计算生物学
生物工程
生物科学
癌症生物学
细胞生物学
更正
发育生物学
药物发现
杂志社论
免疫学
力学生物学
医学
微生物学
分子生物学
神经科学
植物科学
产品文档
撤稿
干细胞
系统生物学
更新

研究物种

节肢动物
鱼类
真菌
无脊椎动物
哺乳纲
其它脊椎动物
植物
原核生物
原生动物
病毒
蠕虫

Peer Reviewed

Preprint

Vendor

Video

相关性
日期
浏览量

Computational Workflow for Genome-Wide DNA Methylation Profiling and Differential Methylation Analysis
全基因组DNA甲基化分析与差异甲基化分析的计算流程

作者：Pei-Yu Lin, Guan-Jun Lin, Kuan-Lin Chen, Shiang-Chin Huang and Pao-Yang Chen，日期：11/05/2025，浏览量：2220，Q&A： 0

...To illustrate the application of BS-seq and EM-seq, we demonstrate a case study on analyzing a mutant...Conversion rateFor methyl-seq (EM-seq and BS

Deaminase-Assisted Sequencing for the Identification of 5-glyceryl-methylcytosine
脱氨酶辅助测序用于鉴定 5-甘油基甲基胞嘧啶

作者：Bao-Dan He, Fan-Chen Wang and Jian-Huang Xue，日期：02/20/2026，浏览量：68，Q&A： 0

...Overall, DEA-seq provides an accessible, efficient, and highly accurate protocol for profiling 5gmC,...In this protocol, DEA-seq was applied to profile 5gmC in the genome of C. re

Reduced Representation Bisulfite Sequencing in Maize
玉米中简化代表性亚硫酸氢盐测序

作者：Fei-Man Hsu, Chung-Ju Rachel Wang and Pao-Yang Chen，日期：03/20/2018，浏览量：9116，Q&A： 0

...These characteristics make targeted bisulfite sequencing (BS-seq) more cost-effective. mCHH islands

Library Preparation for Genome-Wide DNA Methylation Profiling
全基因组DNA甲基化分析的文库构建方法

作者：Fei-Man Hsu, Matteo Pellegrini and Pao-Yang Chen，日期：11/05/2025，浏览量：1587，Q&A： 0

...This process, called bisulfite conversion, is the basis of bisulfite sequencing (BS-seq), which has

circFL-seq, A Full-length circRNA Sequencing Method
circFL-seq，一种全长circRNA的测序方法

作者：Zelin Liu and Ence Yang，日期：04/20/2022，浏览量：4303，Q&A： 1

...of circFL-seq make circRNA reads more than tenfold enriched, and show advantages for identification...Computational pipeline of circFL-seq data.Length distribution of circFL-seq

ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列

作者：Junaid Akhtar, Piyush More and Steffen Albrecht，日期：02/20/2020，浏览量：4732，Q&A： 0

...However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented...bowtie2-x $INDEX_PATH/genome -p 24 -1$SEQ_PATH/*file_1*.gz -2 $SEQ_PATH/*file_2*.

ChIP-seq Experiment and Data Analysis in the Cyanobacterium Synechocystis sp. PCC 6803
蓝藻集胞藻PCC 6803的ChIP-seq实验和数据分析

作者：Joaquín Giner-Lamia, Miguel A. Hernández-Prieto and Matthias E. Futschik，日期：06/20/2018，浏览量：13276，Q&A： 0

...for chromatin immunoprecipitation using NtcA antibodies, followed by DNA sequencing analysis (ChIP-seq...N_ChIP_sorted.bam and N_Input_sorted.bam, respectively, are: SAM to BAM conversion:

Fast TV-PRO-seq: Accelerated and Streamlined Protocol for Timing RNA Polymerase Pausing
Fast TV-PRO-seq：RNA聚合酶停滞时间测定的加速与简化流程

作者：Jie Zhang, Zhixian Liang, Mingxin Sun, Daniel Hebenstreit and Shaohui Zhang，日期：07/20/2025，浏览量：2425，Q&A： 0

...Unlike standard PRO-seq, Fast TV-PRO-seq employs sarkosyl-free biotin-NTP run-on with time gradients...TGGAATTCTCGGGTGCCAAGG Alignment: bowtie2 -k 1 --very-sensitive --no-unal -x

Quantitative ChIP-seq by Adding Spike-in from Another Species
通过引入另一物种的标准参照以定量分析ChIP-seq

作者：Kongyan Niu, Rui Liu and Nan Liu，日期：08/20/2018，浏览量：21845，Q&A： 0

...Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a routine procedure in the lab; however...gt; bamCoverage -b {sample}_ChIP_dm6_Sorted.bam -o {sample}_ChIP_dm6_scaleFacto