• 卷册 11, 2021
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  • 卷册 7, 2017
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  • 卷册 5, 2015
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  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.4160

往期刊物(2021)

卷册: 11 期号: 18

生物化学

Methods for the Extraction of Heme Prosthetic Groups from Hemoproteins 从血红素中提取血红素辅基的方法 作者:Kat Ellis-Guardiola, Jess Soule and Robert T. Clubb日期:09/20/2021,浏览量:851,Q&A: 0

Hemoproteins are widely researched because they contain redox-active heme prosthetic groups (iron + protoporphyrin IX) that enable them to perform a range of vital functions, acting as enzymes, participants in electron transfer reactions, or gas sensing, carrying, and storage proteins. While the heme prosthetic group is almost always essential for

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Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling 利用多聚体谱综合鉴定可翻译环状RNA 作者:Yanwen Ye, Zefeng Wang and Yun Yang日期:09/20/2021,浏览量:764,Q&A: 0

Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many exonic circRNAs, generated by back-splicing of pre-mRNAs, can be translated in a cap-independent

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生物工程

Anaerobic Expression and Purification of Holo-CCIS, an Artificial Iron-sulfur Protein 人工铁硫蛋白 Holo-CCIS的厌氧表达与纯化 作者:Bhanu P. Jagilinki, Irina Paluy, Vikas Nanda and Dror Noy日期:09/20/2021,浏览量:683,Q&A: 0

Iron-sulfur proteins are ubiquitous among all living organisms and are indispensable for almost all metabolic pathways ranging from photosynthesis, respiration, nitrogen, and carbon dioxide cycles. The iron-sulfur clusters primarily serve as electron acceptors and donors and transfer electrons to active sites of various enzymes, thus driving the

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细胞生物学

Isolation of Nasal Brush Cells for Single-cell Preparations 用于单细胞制备的鼻刷细胞的分离 作者:Saltanat Ualiyeva, Amelia A. Boyd, Nora A. Barrett and Lora G. Bankova日期:09/20/2021,浏览量:1198,Q&A: 0

Solitary chemosensory epithelial cells are scattered in most mucosal surfaces. They are referred to as tuft cells in the intestinal mucosa, brush cells in the trachea, and solitary chemosensory and microvillous cells in the nasal mucosa. They are the primary source of IL-25 in the epithelium and are also engaged in acetylcholine generation. We

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发育生物学

En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos 海鞘胚胎中DNA电穿孔的体内转录分析 作者:Sébastien Darras日期:09/20/2021,浏览量:746,Q&A: 0

Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for studying cis-regulatory elements that control gene expression during embryonic development. Here, I

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Inter-species Transplantation of Blastocysts between Medaka and Zebrafish 青鳉与斑马鱼间囊胚的种间移植 作者:Jana F. Fuhrmann, Jasmin Onistschenko and Lázaro Centanin日期:09/20/2021,浏览量:606,Q&A: 0

Transplantation of blastocysts from a donor to a host blastula constitutes a powerful experimental tool to tackle major developmental biology questions. The technique is widely implemented in diverse biological models including teleost fish, where it is typically used for intra-species blastula transplantations – i.e., labeled blastocysts

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免疫学

Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections 人体组织切片中中性粒细胞胞外陷阱的鉴定与定量 作者:Coraline Radermecker, Alexandre Hego, Philippe Delvenne and Thomas Marichal日期:09/20/2021,浏览量:804,Q&A: 0

Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into their environment. Detecting such NETs in humans has often proven challenging for both biological

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微生物学

Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae 无细胞翻译:酿酒酵母翻译活性提取物的制备和验证 作者:Brandon M. Trainor, Anton A. Komar, Dimitri G. Pestov and Natalia Shcherbik日期:09/20/2021,浏览量:734,Q&A: 0

Cell-free translation is a powerful technique for in vitro protein synthesis. While cell-free translation platforms prepared from bacterial, plant, and mammalian cells are commercially available, yeast-based translation systems remain proprietary knowledge of individual labs. Here, we provide a detailed protocol for simple, fast, and

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Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays 葡萄糖饥饿、镁离子饥饿和胆汁应激分析 作者:Aryashree Arunima and Mrutyunjay Suar日期:09/20/2021,浏览量:419,Q&A: 0

Salmonella enterica serovar Enteritidis (S. Enteritidis) is a leading causative pathogen for food-borne gastroenteritis. During its course of infection, it confronts myriads of physiological barriers inside the host, such as nutrient deprivation, low micronutrient availability, and toxicity from bile salts, to promote bacterial survival and

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An Inexpensive Imaging Platform to Record and Quantitate Bacterial Swarming 记录和定量细菌群集的廉价成像平台 作者:Weijie Chen, Sridhar Mani and Jay X. Tang日期:09/20/2021,浏览量:502,Q&A: 0

Bacterial swarming refers to a rapid spread, with coordinated motion, of flagellated bacteria on a semi-solid surface (Harshey, 2003). There has been extensive study on this particular mode of motility because of its interesting biological and physical relevance, e.g., enhanced antibiotic resistance (Kearns, 2010) and turbulent collective motion

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Intrathoracic Inoculation of Zika Virus in Aedes aegypti 埃及伊蚊胸内接种寨卡病毒 作者:Irma Sanchez-Vargas, Adeline E. Williams, Alexander W.E. Franz and Ken E. Olson日期:09/20/2021,浏览量:338,Q&A: 0

Aedes aegypti mosquitoes are the main vectors of many medically relevant arthropod-borne (arbo) viruses, including Zika (ZIKV), dengue (DENV), and yellow fever (YFV). Vector competence studies with Ae. aegypti often involve challenging mosquitoes with an artificial bloodmeal containing virus and later quantifying viral titer or infectious

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分子生物学

Construction of DNA/RNA Triplex Helices Based on GAA/TTC Trinucleotide Repeats 基于GAA/TTC三核苷酸重复序列构建DNA/RNA三链螺旋 作者:Jiahui Zhang, Ashkan Fakharzadeh, Feng Pan, Christopher Roland and Celeste Sagui日期:09/20/2021,浏览量:469,Q&A: 0

Atypical DNA and RNA secondary structures play a crucial role in simple sequence repeat (SSR) diseases, which are associated with a class of neurological and neuromuscular disorders known as “anticipation diseases,” where the age of disease onset decreases and the severity of the disease is increased as the intergenerational expansion

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神经科学

A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin 一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法 作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:441,Q&A: 0

Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact mechanisms underlying its fundamental roles in neuronal growth cones are not fully explored. Recent

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植物科学

Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants 农杆菌介导粳稻成熟胚和再生转基因植株的转化 作者:Ammar Elakhdar, Masako Fukuda and Takahiko Kubo日期:09/20/2021,浏览量:553,Q&A: 0

Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica

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Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing 利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析 作者:Yang Yang and Weixing Shan日期:09/20/2021,浏览量:396,Q&A: 0

Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and chloroplasts, such as poisoned primer extension (PPE) assays, high-resolution melting (HRM) analysis,

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系统生物学

Protocol for RNA-seq Expression Analysis in Yeast 酵母RNA-seq表达分析方法 作者:Stefan Bohn日期:09/20/2021,浏览量:547,Q&A: 0

Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in a few days; however, in particular, data processing, curation, and analysis involve numerous steps

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Assessing the in vitro Binding Specificity of Histone Modification Reader Proteins Using Histone Peptide Arrays 利用组蛋白肽阵列评估组蛋白修饰阅读蛋白的体外结合特异性 作者:Mark W. Soo and Arneet L. Saltzman日期:09/20/2021,浏览量:345,Q&A: 0

In the field of chromatin biology, a major goal of understanding the roles of histone post-translational modifications is to identify the proteins and domains that recognize these modifications. Synthetic histone peptides containing one or more modifications are a key tool to probe these interactions in pull-down assays with recombinant proteins

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