分子生物学

分类

    现刊
    Modeling NOTCH1 driven T-cell Acute Lymphoblastic Leukemia in Mice
    NOTCH1诱导小鼠T细胞急性淋巴细胞白血病模型的建立
    作者:Agnieszka A. Wendorff and Adolfo A. Ferrando日期:05/20/2020,浏览量:435,Q&A: 0
    T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy that arises from transformation of T-cell primed hematopoietic progenitors. Although T-ALL is a heterogenous and molecularly complex disease, more than 65% of ...
    Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography
    Cyanobacterium Synechocystis sp. PCC 6803 中蛋白混合物的标记亲和层析纯化方法
    作者:Minna M. Koskela, Petra Skotnicová, Éva Kiss and Roman Sobotka日期:05/20/2020,浏览量:411,Q&A: 0
    Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are ...
    Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation
    重组输入蛋白-组蛋白配合物分子大小的超离心分析
    作者:Abhilash Padavannil, Chad A. Brautigam and Yuh Min Chook日期:05/20/2020,浏览量:326,Q&A: 0
    Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone ...
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    果蝇IBMPFD模型的建立、分析及体内药物治疗
    作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:318,Q&A: 0
    Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and ...
    In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes
    细菌膜蛋白在蛋白质脂质体中的体外整合
    作者:Hanako Nishikawa, Masaru Sasaki and Ken-ichi Nishiyama日期:05/20/2020,浏览量:508,Q&A: 0
    It is important to experimentally determine how membrane proteins are integrated into biomembranes to unveil the roles of the integration factors, and to understand the functions and structures of membrane proteins. We have developed a ...
    Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
    通过移码荧光蛋白报告基因评估基因组编辑工具的效率
    作者:Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang日期:05/20/2020,浏览量:550,Q&A: 0
    In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription ...
    Modeling NOTCH1 driven T-cell Acute Lymphoblastic Leukemia in Mice
    NOTCH1诱导小鼠T细胞急性淋巴细胞白血病模型的建立
    作者:Agnieszka A. Wendorff and Adolfo A. Ferrando日期:05/20/2020,浏览量:435,Q&A: 0
    [Abstract] T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy that arises from transformation of T-cell primed hematopoietic progenitors. Although T-ALL is a heterogenous and molecularly complex disease, more than 65% of T-ALL patients carry activating mutations in the NOTCH1 gene. The majority of T-ALL–associated ...
    Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography
    Cyanobacterium Synechocystis sp. PCC 6803 中蛋白混合物的标记亲和层析纯化方法
    作者:Minna M. Koskela, Petra Skotnicová, Éva Kiss and Roman Sobotka日期:05/20/2020,浏览量:411,Q&A: 0
    [Abstract] Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are especially notorious. The cyanobacterium Synechocystis sp. PCC 6803 is a widely used model ...
    Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation
    重组输入蛋白-组蛋白配合物分子大小的超离心分析
    作者:Abhilash Padavannil, Chad A. Brautigam and Yuh Min Chook日期:05/20/2020,浏览量:326,Q&A: 0
    [Abstract] Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones. Determining the molecular size of the importin-histone complexes is key to understanding the ...
    Generation, Analyzing and in-vivo Drug Treatment of Drosophila Models with IBMPFD
    果蝇IBMPFD模型的建立、分析及体内药物治疗
    作者:Ting Zhang, Bruce A. Hay and Ming Guo日期:05/20/2020,浏览量:318,Q&A: 0
    [Abstract] Missense mutations of p97/cdc48/Valosin-containing protein (VCP) cause inclusion body myopathy, Paget disease with frontotemporal dementia (IBMPFD) and other neurodegenerative diseases. The pathological mechanism of IBMPFD is not clear and there is no treatment. We generated Drosophila models of IBMPFD in adult flight muscle in ...
    In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes
    细菌膜蛋白在蛋白质脂质体中的体外整合
    作者:Hanako Nishikawa, Masaru Sasaki and Ken-ichi Nishiyama日期:05/20/2020,浏览量:508,Q&A: 0
    [Abstract] It is important to experimentally determine how membrane proteins are integrated into biomembranes to unveil the roles of the integration factors, and to understand the functions and structures of membrane proteins. We have developed a reconstitution system for membrane protein integration in E. coli using purified factors, in which the ...
    Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
    通过移码荧光蛋白报告基因评估基因组编辑工具的效率
    作者:Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang日期:05/20/2020,浏览量:550,Q&A: 0
    [Abstract] In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases (TALEN), and the clustered regularly interspaced short ...
    Confocal and Super-resolution Imaging of RNA in Live Bacteria Using a Fluorogenic Silicon Rhodamine-binding Aptamer
    含氟硅-罗丹明适配体用于活性菌中RNA的激光共聚焦高分辨率成像
    作者:Regina Wirth, Peng Gao, G. Ulrich Nienhaus, Murat Sunbul and Andres Jäschke日期:05/05/2020,浏览量:921,Q&A: 0
    [Abstract] Genetically encoded light-up RNA aptamers have been shown to be promising tools for the visualization of RNAs in living cells, helping us to advance our understanding of the broad and complex life of RNA. Although a handful of light-up aptamers spanning the visible wavelength region have been developed, none of them have yet been reported to be ...
    Split Nano Luciferase-based Assay to Measure Assembly of Japanese Encephalitis Virus
    基于分割纳米荧光素酶的乙型脑炎病毒组装检测
    作者:Simon Goto, Kotaro Ishida, Ryosuke Suzuki and Eiji Morita日期:05/05/2020,浏览量:303,Q&A: 0
    [Abstract] Cells infected with flavivirus release various forms of infectious and non-infectious particles as products and by-products. Comprehensive profiling of the released particles by density gradient centrifugation is informative for understanding viral particle assembly. However, it is difficult to detect low-abundance minor particles in such ...
    Viral Double-Stranded RNA Detection by DNase I and Nuclease S1 digestions in Leishmania parasites
    在利什曼原虫中利用DNase I和 Nuclease S1酶解进行病毒双链RNA鉴定
    作者:Nathalie Isorce and Nicolas Fasel日期:05/05/2020,浏览量:314,Q&A: 0
    [Abstract] Many RNA viruses are found in protozoan parasites. They can be responsible for more serious pathology or treatment failure. For the detection of viral double-stranded RNA (dsRNA), sequence-dependent and -independent methods are available, such as quantitative real-time PCR and immunofluorescence, dot blot, ELISA or sequencing. The technique ...
    Enzymatic Construction of Protein Polymer/Polyprotein Using OaAEP1 and TEV Protease
    利用OaAEP1和TEV蛋白酶进行蛋白聚合物/多聚蛋白的酶促构建
    作者:Yibing Deng, Shengchao Shi, Bin Zheng, Tao Wu and Peng Zheng日期:04/20/2020,浏览量:784,Q&A: 0
    [Abstract] The development of chemical and biological coupling technologies in recent years has made possible of protein polymers engineering. We have developed an enzymatic method for building polyproteins using a protein ligase OaAEP1 (asparagine endopeptidase 1) and protease TEV (tobacco etching virus). Using a mobile TEV protease site compatible with the ...