生物科学

分类

    现刊
    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:0,Q&A: 0

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including

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    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:355,Q&A: 0

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples

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    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    小鼠汗液分析模型对局部出汗区域的客观定量
    作者:Choongjin Ban and Dae-Hyuk Kwoen日期:06/05/2021,浏览量:169,Q&A: 0

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is

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    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

    ...
    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    小鼠汗液分析模型对局部出汗区域的客观定量
    作者:Choongjin Ban and Dae-Hyuk Kwoen日期:06/05/2021,浏览量:169,Q&A: 0
    [Abstract]

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is quantitated by manually counting the number of sweating spots, which can contribute to various

    ...
    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:355,Q&A: 0
    [Abstract]

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    ODELAM:结核分枝杆菌耐药性的快速序列独立检测
    作者:Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison日期:05/20/2021,浏览量:477,Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    Operant Vapor Self-administration in Mice
    小鼠蒸汽吸入自我给药操作
    作者:Renata C. N. Marchette, Brendan J. Tunstall, Leandro F. Vendruscolo and Khaled Moussawi日期:05/20/2021,浏览量:615,Q&A: 0
    [Abstract]

    Models of drug addiction in rodents are instrumental in understanding the underlying neurobiology. Intravenous self-administration of drugs in mice is currently the most commonly used model; however, several challenges exist due to complications related to catheter patency. To take full advantage of the genetic tools available to study opioid

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    曼氏血吸虫感染小鼠的寄生虫病评估
    作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:487,Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves
    年龄、伤口大小和损伤部位 – 生长叶片中依赖于损伤的维管再生试验
    [Abstract]

    Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in

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    COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR
    COVID-19样本汇集:从RNA提取到实时定量RT-PCR
    [Abstract]

    The COVID-19 pandemic requires mass screening to identify those infected for isolation and quarantine. Individually screening large populations for the novel pathogen, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is costly and requires a lot of resources. Sample pooling methods improve the efficiency of mass screening and consume

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    High-throughput Method for Detecting Siderophore Production by Rhizosphere Bacteria
    根际细菌产生铁载体的高通量检测方法
    作者:ShaoHua Gu, Wen Wan, ZhengYing Shao and Wei Zhong日期:05/05/2021,浏览量:420,Q&A: 0
    [Abstract]

    Siderophores, a key substance that microorganisms produce to obtain iron under iron-limited conditions, play an important role in regulating interactions between beneficial bacteria and pathogenic bacteria. A large number of bacteria were isolated from the rhizosphere, and we used the method presented here to assay the siderophore production by

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    Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
    筛选与治疗相关的噬菌体的溶原活性
    作者:Fernando L. Gordillo Altamirano and Jeremy J. Barr日期:04/20/2021,浏览量:1381,Q&A: 1
    [Abstract]

    Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal

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