细胞生物学

分类

    现刊
    Skin Transplantation and Lymphoid Organ Analysis in Mice
    小鼠中皮肤移植和淋巴器官分析
    作者:Julie Ruer-Laventie, Rajesh Jayachandran, Mathias Schmaler, Simona W. Rossi and Jean Pieters日期:02/20/2020,浏览量:180,Q&A: 0
    Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft ...
    Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization
    基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位
    作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:292,Q&A: 0
    Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism ...
    Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry
    利用流式细胞术进行哺乳动物活细胞中Halo 标记蛋白细胞丰度测定
    作者:Claudia Cattoglio, Xavier Darzacq, Robert Tjian and Anders Sejr Hansen日期:02/20/2020,浏览量:404,Q&A: 0
    Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:134,Q&A: 0
    Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. ...
    Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
    小鼠乳腺单个上皮细胞悬液制备
    作者:Heng Sun, Xiaoling Xu and Chuxia Deng日期:02/20/2020,浏览量:74,Q&A: 0
    Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells ...
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task ...
    RNA Sequencing of Single Myofibers from Mus musculus
    小鼠单肌纤维的RNA序列分析
    作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:319,Q&A: 0
    Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but ...
    Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
    大鼠脉络丛原代上皮细胞培养方法
    作者:Valeria Lallai, Amina Ahmed and Christie D. Fowler日期:02/20/2020,浏览量:76,Q&A: 0
    The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:127,Q&A: 0
    Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. ...
    Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation
    骨髓和脂肪间充质干细胞:分离、培养、鉴定和分化
    Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and ...
    Skin Transplantation and Lymphoid Organ Analysis in Mice
    小鼠中皮肤移植和淋巴器官分析
    作者:Julie Ruer-Laventie, Rajesh Jayachandran, Mathias Schmaler, Simona W. Rossi and Jean Pieters日期:02/20/2020,浏览量:180,Q&A: 0
    [Abstract] Skin transplantation in mice is an important procedure to evaluate immune responses generated against heterologous grafts, especially given its highly immunogenic nature. In fact, skin is one of the most challenging organs in terms of allograft retention. In this protocol, we provide a detailed procedure for skin grafting using the tail skin as ...
    Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization
    基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位
    作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:292,Q&A: 0
    [Abstract] Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes in vivo. While recent advances in super-resolution ...
    Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry
    利用流式细胞术进行哺乳动物活细胞中Halo 标记蛋白细胞丰度测定
    作者:Claudia Cattoglio, Xavier Darzacq, Robert Tjian and Anders Sejr Hansen日期:02/20/2020,浏览量:404,Q&A: 0
    [Abstract] Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or require sophisticated experimental and computational infrastructure (e.g., fluorescence ...
    CRISPR-Cas9 Genome Editing of Plasmodium knowlesi
    诺氏疟原虫CRISPR-Cas9基因编辑
    作者:Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon日期:02/20/2020,浏览量:134,Q&A: 0
    [Abstract] Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. falciparum, P. knowlesi is the only other malaria parasite capable of being maintained in ...
    Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
    小鼠乳腺单个上皮细胞悬液制备
    作者:Heng Sun, Xiaoling Xu and Chuxia Deng日期:02/20/2020,浏览量:74,Q&A: 0
    [Abstract] Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    [Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
    RNA Sequencing of Single Myofibers from Mus musculus
    小鼠单肌纤维的RNA序列分析
    作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:319,Q&A: 0
    [Abstract] Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the ...
    Method for Primary Epithelial Cell Culture from the Rat Choroid Plexus
    大鼠脉络丛原代上皮细胞培养方法
    作者:Valeria Lallai, Amina Ahmed and Christie D. Fowler日期:02/20/2020,浏览量:76,Q&A: 0
    [Abstract] The choroid plexus consists of a network of secretory epithelial cells localized throughout the lateral, third and fourth ventricles of the brain. Cerebrospinal fluid (CSF) is generated by the choroid plexus and released into the ventricular environment. This biofluid contains an enriched source of proteins, ions, and other signaling molecules for ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:127,Q&A: 0
    [Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...
    Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation
    骨髓和脂肪间充质干细胞:分离、培养、鉴定和分化
    [Abstract] Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and ...