细胞生物学

分类

    现刊
    Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections
    人体组织切片中中性粒细胞胞外陷阱的鉴定与定量
    作者:Coraline Radermecker, Alexandre Hego, Philippe Delvenne and Thomas Marichal日期:09/20/2021,浏览量:652,Q&A: 0

    Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into

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    Isolation of Nasal Brush Cells for Single-cell Preparations
    用于单细胞制备的鼻刷细胞的分离
    作者:Saltanat Ualiyeva, Amelia A. Boyd, Nora A. Barrett and Lora G. Bankova日期:09/20/2021,浏览量:820,Q&A: 0

    Solitary chemosensory epithelial cells are scattered in most mucosal surfaces. They are referred to as tuft cells in the intestinal mucosa, brush cells in the trachea, and solitary chemosensory and microvillous cells in the nasal mucosa. They are

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    Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections
    人体组织切片中中性粒细胞胞外陷阱的鉴定与定量
    作者:Coraline Radermecker, Alexandre Hego, Philippe Delvenne and Thomas Marichal日期:09/20/2021,浏览量:652,Q&A: 0
    [Abstract]

    Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into their environment. Detecting such NETs in humans has often proven challenging for both biological

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    Isolation of Nasal Brush Cells for Single-cell Preparations
    用于单细胞制备的鼻刷细胞的分离
    作者:Saltanat Ualiyeva, Amelia A. Boyd, Nora A. Barrett and Lora G. Bankova日期:09/20/2021,浏览量:820,Q&A: 0
    [Abstract]

    Solitary chemosensory epithelial cells are scattered in most mucosal surfaces. They are referred to as tuft cells in the intestinal mucosa, brush cells in the trachea, and solitary chemosensory and microvillous cells in the nasal mucosa. They are the primary source of IL-25 in the epithelium and are also engaged in acetylcholine generation. We

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    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:Julie C. Caruana, Scott N. Dean and Scott A. Walper日期:09/05/2021,浏览量:809,Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

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    Cytoduction and Plasmiduction in Yeast
    酵母细胞诱导中的细胞诱导和质粒诱导
    作者:Jane E. Dorweiler and Anita L. Manogaran日期:09/05/2021,浏览量:746,Q&A: 0
    [Abstract]

    Cytoduction, and a related technique referred to as plasmiduction, have facilitated substantial advancements in the field of yeast prion biology by providing a streamlined method of transferring prions from one yeast strain to another. Prions are cytoplasmic elements consisting of aggregated misfolded proteins, and as such, they exhibit

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    Plasmodium cynomolgi Berok Growth Inhibition Assay by Thiol-reactive Probe Based Flow Cytometric Measurement
    硫醇反应探针流式细胞术检测食蟹猴疟原虫生长抑制
    作者:Jessica Jie Ying Ong, Bruce Russell and Jin-Hee Han日期:09/05/2021,浏览量:658,Q&A: 0
    [Abstract]

    The relapsing malaria species, Plasmodium vivax, is the most widely distributed and difficult-to-treat cause of human malaria. The merozoites of P. vivax preferentially invade ephemeral human CD71+ reticulocytes (nascent reticulocytes), thereby limiting the development of a robust continuous culture in vitro. Fortunately, P. vivax’s sister

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    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    体外和体内小鼠γδT细胞的分离和定量
    作者:Isha Rana, Krithika Badarinath, Ravindra K. Zirmire and Colin Jamora日期:09/05/2021,浏览量:821,Q&A: 0
    [Abstract]

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis. The deregulation of this crosstalk can lead to abnormal healing processes and is the foundation of

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    Microscopic Detection of ASC Inflammasomes in Bone Marrow Derived Macrophages Post Stimulation
    刺激后骨髓源性巨噬细胞中ASC炎症小体的显微检测
    作者:Longjun Li, Rudi Mao and Xiaopeng Qi日期:09/05/2021,浏览量:698,Q&A: 0
    [Abstract]

    An inflammasome is an intracellular multiprotein complex that plays important roles in host defense and inflammatory responses. Inflammasomes are typically composed of the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), cytoplasmic sensor protein, and the effector protein pro-caspase-1. ASC assembly into a protein

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    Measuring DNA Damage Using the Alkaline Comet Assay in Cultured Cells
    培养细胞DNA损伤的碱性彗星试验
    作者:Elena Clementi, Zuzana Garajova and Enni Markkanen日期:08/20/2021,浏览量:983,Q&A: 0
    [Abstract]

    Maintenance of DNA integrity is of pivotal importance for cells to circumvent detrimental processes that can ultimately lead to the development of various diseases. In the face of a plethora of endogenous and exogenous DNA-damaging agents, cells have evolved a variety of DNA repair mechanisms that are responsible for safeguarding genetic

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    Isolation of Single Cells from Mouse Periodontal Ligament
    小鼠牙周膜单细胞的分离
    作者:Jing Zhao and Paul Sharpe日期:08/20/2021,浏览量:906,Q&A: 0
    [Abstract]

    The periodontal ligament (PDL) is an essential tissue connecting teeth and bone. It is a complex tissue specifically designed to absorb the forces of mastication; analysis of its multiple cell populations is important to understand its function and the cell changes associated with periodontal disease. Cells in the periodontal ligament are not

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    Preparation of Drosophila Larval Blood Cells for Single-cell RNA Sequencing
    果蝇幼虫血细胞单细胞RNA测序的制备
    作者:Sudhir Gopal Tattikota and Norbert Perrimon日期:08/20/2021,浏览量:546,Q&A: 0
    [Abstract]

    Recent advances in single-cell RNA-sequencing (scRNA-seq) technologies provide unprecedented opportunities to identify new cell types and characterize cell states. One of the most important requirements for performing scRNA-seq is to obtain high-quality single cells in suspension. Recently, we used this approach to characterize Drosophila blood

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