细胞生物学

分类

    现刊
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    阿尔茨海默症幼鼠模型中海马单细胞记录和海马依赖先天性行为
    [Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
    Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH
    利用显色原位杂交进行固定细胞和组织中单个RNA的检测
    作者:Meng Jiang, Chen Lin and Rongqin Ke日期:02/05/2020,浏览量:216,Q&A: 0
    [Abstract] Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence detection. Herein we present a chromogenic in situ hybridization protocol for visualization of ...
    Live Mitochondrial or Cytosolic Calcium Imaging Using Genetically-encoded Cameleon Indicator in Mammalian Cells
    利用基因编码Cameleon指示剂进行哺乳动物细胞中活体线粒体或胞质钙成像
    作者:Elisa Greotti and Tullio Pozzan日期:02/05/2020,浏览量:292,Q&A: 0
    [Abstract] Calcium (Ca2+) imaging aims at investigating the dynamic changes in live cells of its concentration ([Ca2+]) in different pathophysiological conditions. Ca2+ is an ubiquitous and versatile intracellular signal that modulates a large variety of cellular functions thanks to a cell type-specific toolkit and a complex ...
    Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish
    通过斑马鱼视网膜损伤和视网膜组织制备进行视力恢复研究
    作者:Poonam Sharma and Rajesh Ramachandran日期:12/20/2019,浏览量:483,Q&A: 0
    [Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...
    Analysis of EGF Receptor Endocytosis Using Fluorogen Activating Protein Tagged Receptor
    利用荧光团激活蛋白标记受体分析EGF受体的内吞作用
    作者:Mireia Perez Verdaguer, Mads B. Larsen, Marcel P. Bruchez, Simon C. Watkins and Alexander Sorkin日期:12/20/2019,浏览量:541,Q&A: 0
    [Abstract] Functional activities of many transmembrane proteins are controlled by their endocytosis. One of the most studied experimental models is the epidermal growth factor (EGF) receptor (EGFR). However, endocytic trafficking of EGFR has been predominantly analyzed using labeled EGF, whereas quantitative analyses of the endocytosis of the receptor itself ...
    Semi-quantitative Determination of Protein Expression Using Immunohistochemistry Staining and Analysis: An Integrated Protocol
    一种综合的实验方法:利用免疫组织化学染色和分析进行蛋白表达的半定量测定
    作者:Alexandra R Crowe and Wei Yue日期:12/20/2019,浏览量:927,Q&A: 0
    [Abstract] Semi-quantitative IHC is a powerful method for investigating protein expression and localization within tissues. The semi-quantitative immunohistochemistry (IHC) involves using software such as free software ImageJ Fiji to conduct deconvolution and downstream analysis. Currently, there is lack of an integrated protocol that includes a detailed ...
    Quantification of Mitochondrial Dynamics in Fission Yeast
    裂殖酵母内线粒体动力学的定量研究
    作者:Leeba Ann Chacko and Vaishnavi Ananthanarayanan日期:12/05/2019,浏览量:579,Q&A: 0
    [Abstract] Mitochondria are double-membraned organelles responsible for several functions in the cell including energy production, calcium signaling, and cellular metabolism. An equilibrium between fission and fusion events of mitochondria is required for their proper functioning. Mitochondrial morphologies have been quantified in yeast using image ...
    Detection of in vivo Protein Interactions in All Bacterial Compartments by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair
    利用超折叠mTurquoise2 ox-mNeongreen荧光分子共振能量转移法检测细菌体内蛋白相互作用
    作者:Nils Y. Meiresonne, Elisa Consoli, Laureen M.Y. Mertens and Tanneke den Blaauwen日期:12/05/2019,浏览量:652,Q&A: 0
    [Abstract] This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in all compartments of Escherichia coli by Förster Resonance Energy Transfer (FRET) using the Superfolder mTurquoise2 ox-mNeonGreen FRET pair (sfTq2ox-mNG). This FRET pair has more than twice the detection range for FRET ...
    Mapping the Mechanome–A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression
    力学组学映射—一种用于同时进行实时成像和细胞力学适应和侵入定量分析的方案
    [Abstract] Mechanomics, the mechanics equivalent of genomics, is a burgeoning field studying mechanical modulation of stem cell behavior and lineage commitment. Analogous to mechanical testing of a living material as it adapts and evolves, mapping of the mechanome necessitates the development of new protocols to assess changes in structure and function in ...
    Determination of Chromatin Accessibility in Drosophila Midgut Enterocytes by in situ 5mC Labeling
    利用原位5mC标记进行果蝇中肠细胞染色质开放性的鉴定
    作者:Eliya Bitman-Lotan, Hector Rincon-Arano, Gal Raz and Amir Orian日期:11/20/2019,浏览量:548,Q&A: 0
    [Abstract] Regulation of gene expression involves dynamic changes in chromatin organization, where in many cases open chromatin structure correlates with gene activation. Several methods enable monitoring changes in chromatin accessibility, including ATAC-seq, FAIRE-seq, MNase-seq and DNAse-seq methods, which involve Next-generation-sequencing (NGS). ...