免疫学

分类

    现刊
    Identification and Quantitation of Neutrophil Extracellular Traps in Human Tissue Sections
    人体组织切片中中性粒细胞胞外陷阱的鉴定与定量
    作者:Coraline Radermecker, Alexandre Hego, Philippe Delvenne and Thomas Marichal日期:09/20/2021,浏览量:579,Q&A: 0
    [Abstract]

    Neutrophils are one of the first innate immune cells recruited to tissues during inflammation. An important function of neutrophils relies on their ability to release extracellular structures, known as Neutrophil Extracellular Traps or NETs, into their environment. Detecting such NETs in humans has often proven challenging for both biological

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    Isolation and Quantification of Mouse γδT-cells in vitro and in vivo
    体外和体内小鼠γδT细胞的分离和定量
    作者:Isha Rana, Krithika Badarinath, Ravindra K. Zirmire and Colin Jamora日期:09/05/2021,浏览量:798,Q&A: 0
    [Abstract]

    The skin plays an important role in protecting the body from pathogens and chemicals in the external environment. Upon injury, a healing program is rapidly initiated and involves extensive intercellular communication to restore tissue homeostasis. The deregulation of this crosstalk can lead to abnormal healing processes and is the foundation of

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    One-step White Blood Cell Extracellular Staining Method for Flow Cytometry
    流式细胞术白细胞细胞外一步染色法
    作者:Ines Ait Belkacem, Pénélope Bourgoin, Jean Marc Busnel, Franck Galland and Fabrice Malergue日期:08/20/2021,浏览量:683,Q&A: 0
    [Abstract]

    Flow cytometry is a powerful analytical technique that is increasingly used in scientific investigations and healthcare; however, it requires time-consuming, multi-step sample procedures, which limits its use to specialized laboratories. In this study, we propose a new universal one-step method in which white blood cell staining and red blood cell

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    Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer
    多色流式细胞术综合分析乳腺癌小鼠模型中肿瘤免疫渗透
    作者:Ana S. Almeida, Miriam R. Fein and Mikala Egeblad日期:06/05/2021,浏览量:1709,Q&A: 0
    [Abstract]

    Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by

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    A Simple and Robust Protocol for in vitro Differentiation of Mouse Non-pathogenic T Helper 17 Cells from CD4+ T Cells
    一种简单稳定的用小鼠CD4+ T细胞体外分化非致病性T辅助17细胞的方法
    作者:Siwen Kang, Ruohan Wu and Ruoning Wang日期:05/20/2021,浏览量:1973,Q&A: 0
    [Abstract]

    Functional and mechanistic studies of CD4+ T cell lineages rely on robust methods of in vitro T cell polarization. Here, we report an optimized protocol for in vitro differentiation of a mouse non-pathogenic T helper 17 (TH17) cell lineage. Most of the previously established protocols require irradiated splenocytes as artificial antigen presenting

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    A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations
    量化人类和小鼠B细胞亚群中BCR介导的磷酸化的通用实验方法
    作者:Jasper Rip, Rudi W. Hendriks and Odilia B. J. Corneth日期:02/05/2021,浏览量:935,Q&A: 0
    [Abstract]

    Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR

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    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:3095,Q&A: 0
    [Abstract] Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate amounts of ROS act as signaling molecules important for several physiological processes such as regulation ...
    Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
    结核分枝杆菌感染小鼠肺和支气管肺泡灌洗样品中髓样细胞的流式细胞仪分析和荧光激活细胞分选
    作者:Alissa C. Rothchild, Dat Mai, Alan Aderem and Alan H. Diercks日期:05/20/2020,浏览量:3071,Q&A: 0
    [Abstract] Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events of in vivo infection following aerosol challenge is critical for ...
    Assessing in vitro and in vivo Trogocytosis By Murine CD4+ T cells
    用小鼠CD4+T细胞评价体内外胞啃作用
    作者:Jim Reed and Scott A. Wetzel日期:05/05/2020,浏览量:2329,Q&A: 0
    [Abstract] Recognition of antigens by lymphocytes (B, T, and NK) on the surface of an antigen-presenting cell (APC) leads to lymphocyte activation and the formation of an immunological synapse between the lymphocyte and the APC. At the immunological synapse APC membrane and associated membrane proteins can be transferred to the lymphocyte in a process called ...
    Characterization of Immunological Niches within Peyer’s Patches by ex vivo Photoactivation and Flow Cytometry Analysis
    体外光激活和流式细胞术分析派尔斑免疫龛位
    作者:Adi Biram and Ziv Shulman日期:03/20/2020,浏览量:2329,Q&A: 0
    [Abstract] T follicular helper (Tfh) cells regulate B cell selection for entry into the germinal center (GC) reaction or for differentiation into antibody forming cells. This process takes place at the border between the T and B zones in lymphoid organs and involves physical contacts between T and B cells. During these interactions, T cells endow the B cells ...