组织分析 —细胞生物学

Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
小鼠乳腺单个上皮细胞悬液制备

作者：Heng Sun, Xiaoling Xu and Chuxia Deng，日期：02/20/2020，浏览量：74，Q&A： 0

[Abstract] Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...

Bone-in-culture Array to Model Bone Metastasis in ex vivo Condition
体外骨碎片培养列阵模拟骨转移

作者：Hai Wang and Xiang H.-F. Zhang，日期：01/20/2020，浏览量：364，Q&A： 0

[Abstract] Bone is the most frequently affected organ by metastases of breast cancer and prostate cancer. Our knowledge on bone metastasis is extremely limited due to the lack of potent and efficient experimental models. We developed the “Bone-In-Culture Array (BICA)” platform to model the bone colonization of cancer cells in ex vivo cultures. The ...

Characterization of Biological Motion Using Motion Sensing Superpixels
基于运动传感超像素的生物运动特性研究

作者：Felix Y. Zhou, Carlos Ruiz-Puig, Richard P. Owen, Michael J. White, Jens Rittscher and Xin Lu，日期：09/20/2019，浏览量：707，Q&A： 0

[Abstract] Precise spatiotemporal regulation is the foundation for the healthy development and maintenance of living organisms. All cells must correctly execute their function in the right place at the right time. Cellular motion is thus an important dynamic readout of signaling in key disease-relevant molecular pathways. However despite the rapid ...

A Novel Technique for Imaging and Analysis of Hair Cells in the Organ of Corti Using Modified Sca/eS and Machine Learning
利用改进的Sca/eS和机器学习用于螺旋器中毛细胞的成像和分析

作者：Shinji Urata, Tadatsune Iida, Yuri Suzuki, Shiou-Yuh Lin, Yu Mizushima, Chisato Fujimoto, Yu Matsumoto and Tatsuya Yamasoba，日期：08/20/2019，浏览量：931，Q&A： 0

[Abstract] Here, we describe a sorbitol-based optical clearing method, called modified Sca/eS that can be used to image all hair cells (HCs) in the mouse cochlea. This modification of Sca/eS is defined by three steps: decalcification, de-lipidation, and refractive index matching, which can all be completed within 72 h. Furthermore, we established automated ...

Looking through Brains with Fast Passive CLARITY: Zebrafish, Rodents, Non-human Primates and Humans
利用 Fast Passive CLARITY技术研究斑马鱼、啮齿动物、灵长类动物和人类的大脑

作者：Farzad Mortazavi, Alexander J. Stankiewicz and Irina V. Zhdanova，日期：08/05/2019，浏览量：1576，Q&A： 0

[Abstract] Recently developed CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ-hybridization-compatible Tis-sue-hYdrogel) technique renders the tissue transparent by removing lipids in the tissue, while preserving and stabilizing the cellular and subcellular structures. This provides effective penetration of diverse ...

Precision Technique for Splenectomy Limits Mouse Stress Responses for Accurate and Realistic Measurements for Investigating Inflammation and Immunity
脾切除术限制小鼠应激反应的精准技术用于精确现实检测炎症和免疫

作者：Shengwen Calvin Li, Anthony D. Rangel and Mustafa H. Kabeer，日期：08/05/2019，浏览量：948，Q&A： 0

[Abstract] Splenectomy in an animal model requires a standardized technique utilizing best practice to avoid variability which can result in adverse impact to the animal resulting in flawed physiologic responses simply due to technique rather than to the studied variables. In the case of the spleen, often investigators are analyzing the animal immune or ...

Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats
在大鼠切除创面愈合模型中进行伤口愈合的形态学评估

作者：Lígia Reis de Moura Estevão, Puebla Cassini-Vieira, Ana Greice Borba Leite, Apolônia Agnes Vilar de Carvalho Bulhões, Lucíola da Silva Barcelos and Joaquim Evêncio-Neto，日期：07/05/2019，浏览量：1766，Q&A： 0

[Abstract] Skin wound healing is a complex process involving different events such as blood coagulation, inflammation, new blood vessels formation, and extracellular matrix deposition. These events can be observed by using histology techniques. However, the lack of the standardization of such parameters impacts on the reproducibility of results. Here, we ...

The Chick Chorioallantoic Membrane (CAM) Assay as a Three-dimensional Model to Study Autophagy in Cancer Cells
以鸡胚绒毛尿囊膜（CAM）为三维模型研究癌细胞自噬作用

作者：Félice A. Janser, Patricia Ney, Marta Teixeira Pinto, Rupert Langer and Mario P. Tschan，日期：07/05/2019，浏览量：1757，Q&A： 0

[Abstract] The chick chorioallantoic membrane (CAM) is an extra-embryonic organ and thus well accessible for seeding and harvesting 3D cell cultures. Samples from CAM assays are suitable for protein and gene expression analysis as well as for immuno-histochemical studies. Here we present the CAM assay as a possible model to study autophagy in different types ...

Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor
长期间歇性暴露于酒精蒸汽以诱导大鼠酒精依赖

作者：Elizabeth M. Avegno and Nicholas W. Gilpin，日期：05/05/2019，浏览量：1489，Q&A： 0

[Abstract] Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not ...

Immunofluorescence Staining of WT-1/Podocalyxin on Mouse Kidney Sections
免疫荧光标记小鼠肾脏切片中的WT-1/足糖萼

作者：Carole Henique, Olivia Lenoir and Pierre-Louis Tharaux，日期：04/20/2019，浏览量：1335，Q&A： 0

[Abstract] In glomerular diseases, podocytes are one type of cells involved in dysfunction of glomerular filtration. In these conditions, podocyte proteins expression change. Therefore, immunofluorescent staining of podocytes can be performed to visualize podocyte localization of proteins of interest. In this protocol, we detail a method to stain podocytes ...

细胞生物学

Surface Plasmon Resonance Analysis of the Protein-protein Binding Specificity Using Autolab ESPIRIT

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