生物科学

分类

    现刊
    Micrografting in Arabidopsis Using a Silicone Chip
    利用硅芯片进行拟南芥微嫁接
    作者:Hiroki Tsutsui, Yaichi Kawakatsu and Michitaka Notaguchi日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    The micrografting technique in the model plant Arabidopsis has been widely used in the field of plant science. Grafting experiments have demonstrated that signal transductions are systematically regulated in many plant characteristics, including defense mechanisms and responses to surrounding environments such as soil and light conditions.

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    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:355,Q&A: 0
    [Abstract]

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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    Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model
    小鼠汗液分析模型对局部出汗区域的客观定量
    作者:Choongjin Ban and Dae-Hyuk Kwoen日期:06/05/2021,浏览量:169,Q&A: 0
    [Abstract]

    In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is quantitated by manually counting the number of sweating spots, which can contribute to various

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    曼氏血吸虫感染小鼠的寄生虫病评估
    作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:487,Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    Age, Wound Size and Position of Injury – Dependent Vascular Regeneration Assay in Growing Leaves
    年龄、伤口大小和损伤部位 – 生长叶片中依赖于损伤的维管再生试验
    [Abstract]

    Recurring damage to the aerial organs of plants necessitates their prompt repair, particularly their vasculature. While vascular regeneration assays for aerial plant parts such as the stem and inflorescence stalk are well established, those for leaf vasculature remain unexplored. Recently, we established a new vascular regeneration assay in

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    Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole
    甲巯咪唑单次或多次消融小鼠嗅上皮细胞
    作者:Sofia Håglin, Staffan Bohm and Anna Berghard日期:04/20/2021,浏览量:790,Q&A: 0
    [Abstract]

    Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

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    Development of a Chemical Reproductive Aging Model in Female Rats
    雌性大鼠化学生殖衰老模型的建立
    [Abstract]

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from reproductive to non-reproductive life. It is usually characterized by neuroendocrine, metabolic and

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    Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
    筛选与治疗相关的噬菌体的溶原活性
    作者:Fernando L. Gordillo Altamirano and Jeremy J. Barr日期:04/20/2021,浏览量:1381,Q&A: 1
    [Abstract]

    Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal

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    Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction
    采用败血症盲肠结扎穿刺模型诱导大鼠多脏器功能障碍
    [Abstract]

    Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans.

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    Liposomal Clodronate-mediated Macrophage Depletion in the Zebrafish Model
    在斑马鱼模型中脂质体氯膦酸盐介导的巨噬细胞耗竭
    作者:Linlin Yang, Alison M. Rojas and Celia E. Shiau日期:03/20/2021,浏览量:1143,Q&A: 0
    [Abstract]

    The ability to conduct in vivo macrophage-specific depletion remains an effective means to uncover functions of macrophages in a wide range of physiological contexts. Compared to the murine model, zebrafish offer superior imaging capabilities due to their optical transparency starting from a single-cell stage to throughout larval development.

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