生物科学

分类

    现刊
    Inter-species Transplantation of Blastocysts between Medaka and Zebrafish
    青鳉与斑马鱼间囊胚的种间移植
    作者:Jana F. Fuhrmann, Jasmin Onistschenko and Lázaro Centanin日期:09/20/2021,浏览量:461,Q&A: 0
    [Abstract]

    Transplantation of blastocysts from a donor to a host blastula constitutes a powerful experimental tool to tackle major developmental biology questions. The technique is widely implemented in diverse biological models including teleost fish, where it is typically used for intra-species blastula transplantations – i.e., labeled blastocysts

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    Intrathoracic Inoculation of Zika Virus in Aedes aegypti
    埃及伊蚊胸内接种寨卡病毒
    作者:Irma Sanchez-Vargas, Adeline E. Williams, Alexander W.E. Franz and Ken E. Olson日期:09/20/2021,浏览量:233,Q&A: 0
    [Abstract]

    Aedes aegypti mosquitoes are the main vectors of many medically relevant arthropod-borne (arbo) viruses, including Zika (ZIKV), dengue (DENV), and yellow fever (YFV). Vector competence studies with Ae. aegypti often involve challenging mosquitoes with an artificial bloodmeal containing virus and later quantifying viral titer or infectious

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    A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin
    一种高效生产和Src激酶介导的马海兔磷酸化的共纯化方法
    作者:Sherlene L. Brown, Yuan Ren, Daniel M. Suter and Seema Mattoo日期:09/20/2021,浏览量:286,Q&A: 0
    [Abstract]

    Cortactin is an actin-binding protein that regulates processes like cell migration, endocytosis, and tumor cell metastasis. Although cortactin is associated with actin-cytoskeletal dynamics in non-neuronal cells and cell-free systems, the exact mechanisms underlying its fundamental roles in neuronal growth cones are not fully explored. Recent

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    In vitro Cleavage and Electrophoretic Mobility Shift Assays for Very Fast CRISPR
    快速CRISPR的体外裂解和电泳迁移率位移测定
    作者:Roger S. Zou, Yang Liu and Taekjip Ha日期:09/05/2021,浏览量:735,Q&A: 0
    [Abstract]

    CRISPR-Cas9 has transformed biomedical research and medicine through convenient and targeted manipulation of DNA. Time- and spatially-resolved control over Cas9 activity through the recently developed very fast CRISPR (vfCRISPR) system have facilitated comprehensive studies of DNA damage and repair. Understanding the fundamental principles of Cas9

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    Using Atomic Force Microscopy to Study the Real Time Dynamics of DNA Unwinding by Mitochondrial Twinkle Helicase
    使用原子力显微镜研究线粒体闪烁解旋酶对DNA解旋的实时动力学
    作者:Parminder Kaur, Hai Pan, Matthew J. Longley, William C. Copeland and Hong Wang日期:09/05/2021,浏览量:797,Q&A: 0
    [Abstract]

    Understanding the structure and dynamics of DNA-protein interactions during DNA replication is crucial for elucidating the origins of disorders arising from its dysfunction. In this study, we employed Atomic Force Microscopy as a single-molecule imaging tool to examine the mitochondrial DNA helicase Twinkle and its interactions with DNA. We used

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    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:Julie C. Caruana, Scott N. Dean and Scott A. Walper日期:09/05/2021,浏览量:809,Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

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    Cytoduction and Plasmiduction in Yeast
    酵母细胞诱导中的细胞诱导和质粒诱导
    作者:Jane E. Dorweiler and Anita L. Manogaran日期:09/05/2021,浏览量:746,Q&A: 0
    [Abstract]

    Cytoduction, and a related technique referred to as plasmiduction, have facilitated substantial advancements in the field of yeast prion biology by providing a streamlined method of transferring prions from one yeast strain to another. Prions are cytoplasmic elements consisting of aggregated misfolded proteins, and as such, they exhibit

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    Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish
    成年斑马鱼肌纤维的分离和肌肉干细胞的培养
    作者:Massimo Ganassi, Peter S. Zammit and Simon M. Hughes日期:09/05/2021,浏览量:1255,Q&A: 0
    [Abstract]

    Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes to both muscle growth and regeneration. Upon exposure to activating signals, MuSCs proliferate to

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    Tomato Stem Injection for the Precise Assessment of Ralstonia solanacearum Fitness in Planta
    番茄茎杆注射法精确评价青枯菌在植物中的适应性
    作者:Yaru Wang, Liu Xian, Gang Yu and Alberto P. Macho日期:08/20/2021,浏览量:480,Q&A: 0
    [Abstract]

    Ralstonia solanacearum is a soil-borne pathogen with worldwide distribution that causes bacterial wilt disease in more than 250 plant species. R. solanacearum invades plants through the roots, reaches the vascular system, and colonizes the whole plant by moving through the xylem, where it eventually replicates rapidly, causing plant death. Usual

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    Isolation and in vitro Culture of Mouse Oocytes
    小鼠卵母细胞的分离与体外培养
    作者:Jessica Greaney, Goutham Narayanan Subramanian, Yunan Ye and Hayden Homer日期:08/05/2021,浏览量:912,Q&A: 0
    [Abstract]

    Females are endowed at birth with a fixed reserve of oocytes, which declines both in quantity and quality with advancing age. Understanding the molecular mechanisms regulating oocyte quality is crucial for improving the chances of pregnancy success in fertility clinics. In vitro culture systems enable researchers to analyse important molecular and

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