生物化学

分类

    现刊
    Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
    蛋白质-蛋白质复合物的动力学分析,以确定其电离常数 (KD) 和使用生物层干涉法相互作用的效应分子的有效浓度 (EC50)
    作者:Tim Orthwein, Luciano F. Huergo, Karl Forchhammer and Khaled A. Selim日期:09/05/2021,浏览量:1193,Q&A: 0
    [Abstract]

    Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a 6×His tagged fusion protein as bait immobilized on the NTA sensor chip of the

    ...
    Oil Red O Staining for Lipid Content in Caenorhabditis elegans
    油红O染色测定秀丽隐杆线虫脂质含量  
    作者:Feng-Yung Wang and Tsui-Ting Ching日期:08/20/2021,浏览量:1029,Q&A: 0
    [Abstract]

    The nematode Caenorhabditis elegans has emerged as a popular model system for studying the regulation of lipid metabolism. Therefore, it is critical to develop a method for determining fat storage in individual worms. Oil Red O (ORO) staining has been validated as an accurate assessment for major fat storage in C. elegans. Here, we describe an

    ...
    Yeast Lipid Extraction and Analysis by HPTLC
    酵母脂质的HPTLC提取及分析
    作者:Dan Li, Zheng-Tan Zhang, Cheng-Wen He and Zhiping Xie日期:07/05/2021,浏览量:1229,Q&A: 0
    [Abstract]

    The diversity of lipid structures, properties, and combinations in biological tissues makes their extraction and analysis an experimental challenge. Accordingly, even for one of the simplest single-cellular fungi, the budding yeast (Saccharomyces cerevisiae), numerous extraction and analysis protocols have been developed to separate and quantitate

    ...
    Extraction and Quantification of Sphingolipids from Hemiptera Insects by Ultra-Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry
    超高效液相色谱-串联质谱联用法提取和定量半翅目昆虫鞘脂质
    作者:Ni Wang, Xiaoxiao Shi, Chao Zhang, Wenwu Zhou and Zeng-Rong Zhu日期:02/20/2021,浏览量:2391,Q&A: 0
    [Abstract]

    Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis.

    ...
    Using 14C-acetate Pulse-chase Labeling to Study Fatty Acid and Glycerolipid Metabolism in Plant Leaves
    用14C -乙酸酯脉冲追逐标记法研究植物叶片中脂肪酸和甘油脂代谢
    作者:Linhui Yu, Chao Zhou, Jilian Fan and Changcheng Xu日期:02/05/2021,浏览量:1747,Q&A: 0
    [Abstract]

    Lipids metabolism is comprised of networks of reactions occurred in different subcellular compartments. Isotopic labeling is a good way to track the transformations and movements of metabolites without perturbing overall cellular metabolism. Fatty acids, the building blocks of membrane lipids and storage triacylglycerols, are synthesized in

    ...
    Triacylglycerol Measurement in HeLa Cells
    HeLa细胞中三酰甘油的测定
    作者:Ximing Du and Hongyuan Yang日期:12/20/2020,浏览量:1575,Q&A: 0
    [Abstract]

    Lipid droplets store triacylglycerols (triglycerides) and sterol esters to regulate lipid and energy homeostasis. Triacylglycerol measurement is often performed during the investigation of lipid droplet formation and growth. This protocol describes a reliable method using a fluorometric lipid quantification kit to measure triacylglycerols

    ...
    Total Triglyceride Quantification in Caenorhabditis elegans
    秀丽隐杆线虫总甘油三酯的定量研究
    作者:Anjali Sandhu and Varsha Singh日期:11/20/2020,浏览量:1985,Q&A: 0
    [Abstract] Several studies suggest an important role of lipid metabolism in regulating longevity of Caenorhabditis elegans. Therefore, assays to quantify lipids have enormous value in understanding aging and pathologies associated with it. Approximately 70% of lipid metabolism genes in the nematode have orthologs in humans. Amenability of C. elegans ...
    Generation of Giant Unilamellar Vesicles (GUVs) Using Polyacrylamide Gels
    使用聚丙烯酰胺凝胶生成巨型磷脂囊泡(GUVs)
    作者:Eric Parigoris, Daniel L. Dunkelmann and Unai Silvan日期:11/05/2020,浏览量:2054,Q&A: 0
    [Abstract] Giant unilamellar vesicles (GUVs) are a widely used model system for a range of applications including membrane biophysics, drug delivery, and the study of actin dynamics. While several protocols have been developed for their generation in recent years, the use of these techniques involving charged lipid types and buffers of physiological ionic ...
    Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein
    钙黄绿素释放法测定重组α-突触核蛋白膜通透性
    作者:Sayan Dutta, Ben G. Watson, Seema Mattoo and Jean-Christophe Rochet日期:07/20/2020,浏览量:2987,Q&A: 0
    [Abstract] Lipid membranes are involved in regulating biochemical and biological processes and in modulating the selective permeability of cells, organelles, and vesicles. Membrane composition, charge, curvature, and fluidity all have concerted effects on cellular signaling and homeostasis. The ability to prepare artificial lipid assemblies that mimic ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:2550,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...