发育生物学

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    现刊
    Fe-NTA Microcolumn Purification of Phosphopeptides from Immunoprecipitation (IP) Eluates for Mass Spectrometry Analysis
    Fe-NTA微柱纯化免疫沉淀(IP)洗脱物中磷酸肽的质谱分析
    作者:Ethan J. Sanford and Marcus B. Smolka日期:08/05/2021,浏览量:503,Q&A: 0
    [Abstract]

    Protein phosphorylation is a nearly universal signaling mechanism. To date, a number of proteomics tools have been developed to analyze phosphorylation. Phosphoproteome-wide analyses using whole cell extracts suffer from incomplete coverage, often missing phosphorylation events from low-abundance proteins. In order to increase coverage of

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    Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
    果蝇翅成虫上皮中Wg运输的可视化和定量研究
    作者:Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross日期:06/05/2021,浏览量:1398,Q&A: 0
    [Abstract]

    Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the endosomal pathway. Long-range Wg secretion and spread depend on secondary secretion from endosomal

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    Imaging and Fluorescence Quantification in Caenorhabditis elegans with Flow Vermimetry and Automated Microscopy
    秀丽隐杆线虫的流动蠕虫仪和自动显微镜成像和荧光定量研究
    作者:Elissa Tjahjono, Alexey V. Revtovich and Natalia V. Kirienko日期:05/20/2021,浏览量:1797,Q&A: 0
    [Abstract]

    Gene activation and cellular biomarkers are commonly monitored using fluorescent signals from transgenic reporters or dyes. These quantifiable markers are critical for biological research and serve as an incredibly powerful tool, even more so when combined with high-throughput screening. Caenorhabditis elegans is a particularly useful model in

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    Analysis of TORC1-body Formation in Budding Yeast
    芽殖酵母TORC1-body形成的分析
    作者:Ryan L. Wallace, Eric Lu, Arron Sullivan, James E. Hughes Hallett and Andrew P. Capaldi日期:04/05/2021,浏览量:1976,Q&A: 0
    [Abstract]

    The Target of Rapamycin kinase Complex I (TORC1) is the master regulator of cell growth and metabolism in eukaryotes. In the presence of pro-growth hormones and abundant nutrients, TORC1 is active and drives protein, lipid, and nucleotide synthesis by phosphorylating a wide range of proteins. In contrast, when nitrogen and/or glucose levels fall,

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    Ligand and Carbohydrate Engagement (LACE) Assay and Fluorescence Quantification on Murine Neural Tissue
    小鼠神经组织中配体和碳水化合物结合(LACE)实验和荧光定量测定
    作者:James M. Clegg and Thomas Pratt日期:03/20/2021,浏览量:2222,Q&A: 0
    [Abstract]

    The interaction between cell surface heparan sulphate and diffusible ligands such as FGFs is of vital importance for downstream signaling, however, there are few techniques that can be used to investigate this binding event. The ligand and carbohydrate engagement (LACE) assay is a powerful tool which can be used to probe the molecular interaction

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    Molecular and Phenotypic Characterization Following RNAi Mediated Knockdown in Drosophila
    RNAi介导的基因敲除后果蝇的分子和表型特征
    作者:Saurabh Jayesh Kumar Mehta, Pradyumna A. Joshi and Ram Kumar Mishra日期:02/20/2021,浏览量:2054,Q&A: 0
    [Abstract]

    Loss of function studies shed significant light on the involvement of a gene or gene product in different cellular processes. Short hairpin RNA (shRNA) mediated RNA interference (RNAi) is a classical yet straightforward technique frequently used to knock down a gene for assessing its function. Similar perturbations in gene expression can be

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    DigiTAG–a RNA Sequencing Approach to Analyze Transcriptomes of Rare Cell Populations in Drosophila melanogaster
    DigiTAG–一种分析果蝇稀有细胞群转录组的RNA测序方法
    作者:Lisa Landskron, Francois Bonnay, Thomas R. Burkard and Jürgen A. Knoblich日期:11/05/2020,浏览量:1609,Q&A: 0
    [Abstract]

    Cell-type specific transcriptional programs underlie the development and maintenance of organs. Not only distinct cell types within a tissue, even cells with supposedly identical cell fates show a high degree of transcriptional heterogeneity. Inevitable, low cell numbers are a major hurdle to study transcriptomes of pure cell populations. Here we

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    Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus
    水云属褐藻配子体-孢子体发育重编程扩散因子的产生及生物测定
    [Abstract] The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
    In vitro Induction and Detection of Acrosomal Exocytosis in Human Spermatozoa
    人精子中顶体胞吐作用的体外诱导和检测
    作者:Shenae L. Cafe, Amanda L. Anderson and Brett Nixon日期:07/20/2020,浏览量:1768,Q&A: 0
    [Abstract] The acrosome reaction is a highly regulated exocytotic event that primes spermatozoa for successful fertilization. Upon induction, acrosomal exocytosis proceeds via a wave of vesiculation that radiates across the sperm head, destabilizing the acrosomal vesicle and resulting in the release of the acrosomal contents. Having shed their acrosome, ...
    Organotypic Slice Culture of the Embryonic Mouse Brain
    胚胎小鼠大脑的器官型切片培养
    作者:James M. Clegg and Thomas Pratt日期:07/05/2020,浏览量:1738,Q&A: 0
    [Abstract] Organotypic slice culture is a powerful technique for exploring the embryonic development of the mammalian brain. In this protocol we describe a basic slice culture technique we have used for two sets of experiments: axon guidance transplant assays and bead culture assays.