微生物学

分类

    现刊
    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:Stefan Bohn日期:09/20/2021,浏览量:357,Q&A: 0
    [Abstract]

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in a few days; however, in particular, data processing, curation, and analysis involve numerous steps

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    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    酸水解法提取古生菌核心脂质及HPLC-MS分析
    作者:Maxime Tourte, Philippe Schaeffer, Vincent Grossi and Philippe M. Oger日期:08/20/2021,浏览量:391,Q&A: 0
    [Abstract]

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to extreme conditions, which is now widely accepted to stem from the unique structure of their lipids. While

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    Monitoring Protein Splicing Using In-gel Fluorescence Immediately Following SDS-PAGE
    SDS-PAGE后立即使用凝胶内荧光监测蛋白质剪接
    作者:Joel Weinberger II and Christopher W. Lennon日期:08/20/2021,浏览量:1179,Q&A: 0
    [Abstract]

    Inteins garner significant interest from both basic and applied researchers due to their unique catalytic abilities. Herein, we describe a protocol for accurately monitoring protein splicing without purification using in-gel fluorescence immediately following Tris-Glycine SDS-PAGE. Following expression in Escherichia coli, cells are lysed by

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    Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies
    体外选择单体的高度多样性mRNA文库的构建
    [Abstract]

    Recently, we developed transcription/translation coupled with the association of puromycin linker (TRAP) display as a quick in vitro selection method to obtain antibody-like proteins. For the in vitro selection, it is important to prepare mRNA libraries among which the diversity is high. Here, we describe a method for the preparation of monobody

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    植物线粒体核糖体的纯化和低温电镜分析
    作者:Florent Waltz, Philippe Giegé and Yaser Hashem日期:08/05/2021,浏览量:794,Q&A: 0
    [Abstract]

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among mitochondrial gene expression processes, translation is the least understood; although, recent advances

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    In vitro Nitrate Reductase Activity Assay of Mycolicibacterium smegmatis Crude Extract
    耻垢分枝杆菌粗提物硝酸还原酶活性的体外测定
    作者:Wei Tan, Zhi-Hui Shao and Guo-Ping Zhao日期:07/20/2021,浏览量:633,Q&A: 0
    [Abstract]

    Nitrate is one of the major inorganic nitrogen sources for microorganisms. Many bacterial and archaeal lineages can express cytoplasmic assimilatory nitrate reductase (NAS), which catalyzes the rate-limiting reduction of nitrate to nitrite in the nitrate assimilation pathway. Here, we present a detailed protocol for measuring in vitro nitrate

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    Yeast Lipid Extraction and Analysis by HPTLC
    酵母脂质的HPTLC提取及分析
    作者:Dan Li, Zheng-Tan Zhang, Cheng-Wen He and Zhiping Xie日期:07/05/2021,浏览量:1139,Q&A: 0
    [Abstract]

    The diversity of lipid structures, properties, and combinations in biological tissues makes their extraction and analysis an experimental challenge. Accordingly, even for one of the simplest single-cellular fungi, the budding yeast (Saccharomyces cerevisiae), numerous extraction and analysis protocols have been developed to separate and quantitate

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    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
    作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:2218,Q&A: 0
    [Abstract]

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

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    Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins
    评估粘蛋白与细菌和细菌蛋白结合的实验
    作者:Lubov S. Grigoryeva, Saima Rehman, Richard C. White, James A. Garnett and Nicholas P. Cianciotto日期:03/05/2021,浏览量:1576,Q&A: 0
    [Abstract]

    Legionella pneumophila, a Gram-negative bacterium and the causative agent of Legionnaires’ disease, exports over 300 effector proteins/virulence factors, through its type II (T2SS) and type IV secretion systems (T4SS). One such T2SS virulence factor, ChiA, not only functions as a chitinase, but also as a novel mucinase, which we believe aids

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    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:2467,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

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