微生物学

分类

    现刊
    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
    作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:628,Q&A: 0
    [Abstract]

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

    ...
    Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins
    评估粘蛋白与细菌和细菌蛋白结合的实验
    作者:Lubov S. Grigoryeva, Saima Rehman, Richard C. White, James A. Garnett and Nicholas P. Cianciotto日期:03/05/2021,浏览量:1175,Q&A: 0
    [Abstract]

    Legionella pneumophila, a Gram-negative bacterium and the causative agent of Legionnaires’ disease, exports over 300 effector proteins/virulence factors, through its type II (T2SS) and type IV secretion systems (T4SS). One such T2SS virulence factor, ChiA, not only functions as a chitinase, but also as a novel mucinase, which we believe aids

    ...
    Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy
    用原子力显微镜描述DNA解旋酶PriA与停止的DNA复制叉的相互作用
    作者:Yaqing Wang, Zhiqiang Sun, Piero R. Bianco and Yuri L. Lyubchenko日期:03/05/2021,浏览量:1348,Q&A: 0
    [Abstract]

    In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding protein (SSB) is typically present at the abandoned forks,

    ...
    Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids
    受荧光标记和易于固化质粒影响的假单胞菌快速基因组工程
    作者:Daniel C. Volke, Nicolas T. Wirth and Pablo I. Nikel日期:02/20/2021,浏览量:2213,Q&A: 0
    [Abstract]

    Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted methods, homologous recombination, or λ

    ...
    Preparation of Bacterial Outer Membrane Vesicles for Characterisation of Periplasmic Proteins in Their Native Environment
    细菌外膜囊泡的制备及其原生环境中周质蛋白的表征
    作者:Johannes Thoma and Björn M. Burmann日期:12/20/2020,浏览量:1350,Q&A: 0
    [Abstract]

    Bacterial outer membrane vesicles (OMVs) are naturally formed by budding from the outer membrane of Gram-negative bacteria. OMVs consist of a lipid bilayer identical in composition to the original outer membrane and contain periplasmic content within their lumen. Enriched with specific envelope proteins, OMVs make for an excellent native-like

    ...
    Expression and Purification of Recombinant Skd3 (Human ClpB) Protein and Tobacco Etch Virus (TEV) Protease from Escherichia coli
    重组Skd3(人ClpB)蛋白和烟草蚀刻病毒(TEV)蛋白酶在大肠杆菌中的表达与纯化
    作者:Ryan R. Cupo and James Shorter日期:12/05/2020,浏览量:1423,Q&A: 0
    [Abstract]

    Skd3 (encoded by human CLPB) is a mitochondrial AAA+ protein comprised of an N-terminal ankyrin-repeat domain and a C-terminal HCLR-clade nucleotide-binding domain. The function of Skd3 has long remained unknown due to challenges in purifying the protein to high quality and near homogeneity. Recently we described Skd3 as a human mitochondrial

    ...
    Charging State Analysis of Transfer RNA from an α-proteobacterium
    α-变形杆菌转运RNA的装载状态分析
    作者:Liang Yin and Caroline S. Harwood日期:12/05/2020,浏览量:838,Q&A: 0
    [Abstract] Transfer RNA (tRNA) is an essential link between the genetic code and proteins. During the process of translation, tRNA is charged with its cognate amino acid and delivers it to the ribosome, thus serving as a substrate of protein synthesis. To analyze the charging state of a particular tRNA, total RNA is purified and analyzed on an acid-urea gel. ...
    Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot
    α-变形杆菌核糖体的纯化及Northern印迹分析
    作者:Liang Yin and Caroline S. Harwood日期:12/05/2020,浏览量:818,Q&A: 0
    [Abstract] Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we describe how to purify ribosomes from the α-proteobacterium Rhodopseudomonas palustris ...
    Combining Gel Retardation and Footprinting to Determine Protein-DNA Interactions of Specific and/or Less Stable Complexes
    结合凝胶阻滞和足印法测定特异性和/或不稳定复合物的蛋白质-DNA相互作用
    作者:Meng-Lun Hsieh, Alice Boulanger, Leslie G. Knipling and Deborah M. Hinton日期:12/05/2020,浏览量:853,Q&A: 0
    [Abstract]

    DNA footprinting is a classic technique to investigate protein-DNA interactions. However, traditional footprinting protocols can be unsuccessful or difficult to interpret if the binding of the protein to the DNA is weak, the protein has a fast off-rate, or if several different protein-DNA complexes are formed. Our protocol differs from traditional

    ...
    In vitro Glutamylation Inhibition of Ubiquitin Modification and Phosphoribosyl-Ubiquitin Ligation Mediated by Legionella pneumophila Effectors
    嗜肺军团菌介导的泛素修饰和磷酸核糖泛素连接的体外谷氨酰化抑制
    作者:Alan G. Sulpizio, Marena E. Minelli and Yuxin Mao日期:11/05/2020,浏览量:864,Q&A: 0
    [Abstract]

    Glutamylation is a posttranslational modification where the amino group of a free glutamate amino acid is conjugated to the carboxyl group of a glutamate side chain within a target protein. SidJ is a Legionella kinase-like protein that has recently been identified to perform protein polyglutamylation of the Legionella SdeA Phosphoribosyl-Ubiquitin

    ...