细胞生物学

分类

    现刊
    Fixation and Immunostaining of Endogenous Proteins or Post-translational Modifications in Caenorhabditis elegans
    秀丽隐杆线虫内源性蛋白质的固定和免疫染色或翻译后修饰
    作者:Robert O'Hagan and Irini Topalidou日期:10/05/2021,浏览量:726,Q&A: 0
    [Abstract]

    Although the advent of genetically-encoded fluorescent markers, such as the green fluorescent protein (GFP; Chalfie et al., 1994), has enabled convenient visualization of gene expression in vivo, this method is generally not effective for detecting post-translational modifications because they are not translated from DNA sequences.

    ...
    Preparation of Human Chondrocytes for Profiling Using Cytometry by Time-of-flight (cyTOF)
    用飞行时间(cyTOF flight)流式细胞术制备人软骨细胞用于分析研究
    作者:Fiorella Carla Grandi and Nidhi Bhutani日期:07/20/2021,浏览量:1262,Q&A: 0
    [Abstract]

    Single-cell technologies have allowed high-resolution profiling of tissues and thus a deeper understanding of tissue homeostasis and disease heterogeneity. Understanding this heterogeneity can be especially important for tailoring treatments in a patient-specific manner. Here, we detail methods for preparing human cartilage tissue for profiling

    ...
    Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy
    用免疫荧光显微镜追踪金黄色葡萄球菌表面蛋白的亚细胞定位
    作者:Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu日期:05/20/2021,浏览量:3484,Q&A: 0
    [Abstract]

    Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal membrane at mid-cell, anchored to the cell wall peptidoglycan at the cross-wall compartment,

    ...
    Molecular and Phenotypic Characterization Following RNAi Mediated Knockdown in Drosophila
    RNAi介导的基因敲除后果蝇的分子和表型特征
    作者:Saurabh Jayesh Kumar Mehta, Pradyumna A. Joshi and Ram Kumar Mishra日期:02/20/2021,浏览量:2145,Q&A: 0
    [Abstract]

    Loss of function studies shed significant light on the involvement of a gene or gene product in different cellular processes. Short hairpin RNA (shRNA) mediated RNA interference (RNAi) is a classical yet straightforward technique frequently used to knock down a gene for assessing its function. Similar perturbations in gene expression can be

    ...
    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    两个不同植物谱系根内真菌结构的酸化蓝墨水染色法观察流程
    作者:Jill Kowal, Elena Arrigoni and Sophie Lane日期:10/20/2020,浏览量:1565,Q&A: 0
    [Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...
    Double Labeling of PDGFR-β and α-SMA in Swine Models of Acute Kidney Injury to Detect Pericyte-to-Myofibroblast Transdifferentation as Early Marker of Fibrosis
    双标记PDGFR-β和α-SMA在猪急性肾损伤模型中检测周细胞-肌成纤维细胞转化作为早期纤维化标志物的研究
    [Abstract] Growing evidences suggest that peritubular capillaries pericytes are the main source of scar-forming myofibroblasts during chronic kidney disease (CKD), as well as early phases of acute kidney injury (AKI). In a swine model of sepsis and I/R (Ischemia Reperfusion) injury-induced AKI we demonstrated that renal pericytes are able to ...
    Immunofluorescent Staining of Claudin-2 in Cultured Kidney Tubular Cells
    肾脏肾小管培养细胞中Claudin-2的免疫荧光染色
    作者:Shaista Anwer and Katalin Szaszi日期:07/20/2020,浏览量:1802,Q&A: 0
    [Abstract] Members of the claudin family of tight junction proteins regulate paracellular permeability and modulate cell signaling. During junction remodeling, these proteins are selectively inserted into or retrieved from the tight junctions, but the control and coordination of these processes remain incompletely understood. Visualization of claudins allows ...
    Microscopy-based Methods for Rosetting Assay in Malaria Research
    基于显微技术的疟疾玫瑰花环试验
    作者:Wenn-Chyau Lee and Laurent Rénia日期:07/05/2020,浏览量:2273,Q&A: 0
    [Abstract] In malaria, rosetting phenomenon is a condition where a Plasmodium-infected erythrocyte stably adheres to at least an uninfected erythrocyte. This phenomenon that occurs in all species of human malaria parasite is likely to be an immune escape mechanism for the parasite. However, it has been associated with malaria pathogenesis, possibly ...
    Testing Bone Formation Induction by Calvarial Injection Assay in vivo
    体内颅骨注射分析检测骨形成
    作者:Narelle E McGregor, Ingrid J Poulton, Emma C Walker and Natalie A Sims日期:03/20/2020,浏览量:2604,Q&A: 0
    [Abstract] Bone formation occurs during embryogenesis, skeletal growth and during the process of skeletal renewal throughout life. In the process of bone formation, osteoblasts lay down a collagen-containing matrix, termed osteoid, which is gradually hardened by incorporation of mineral crystals. Although osteoblasts can be induced to differentiate and to ...
    Manganese Superoxide Dismutase Activity Assay in the Yeast Saccharomyces cerevisiae
    啤酒酵母中的锰超氧化物歧化酶活性测定
    作者:Louise Thines and Pierre Morsomme日期:03/05/2020,浏览量:1989,Q&A: 0
    [Abstract] Superoxide dismutases (SODs) act as a primary defence against reactive oxygen species (ROS) by converting superoxide anion radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2). Members of this enzyme family include CuZnSODs, MnSODs, FeSODs, and NiSODs, depending on the nature ...