癌症生物学

分类

    现刊
    Comprehensive Identification of Translatable Circular RNAs Using Polysome Profiling
    利用多聚体谱综合鉴定可翻译环状RNA
    作者:Yanwen Ye, Zefeng Wang and Yun Yang日期:09/20/2021,浏览量:38,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs), a special type of RNAs without 5’- and 3’-ends, are widely present in eukaryotes and known to function as noncoding RNAs to regulate gene expression, including as miRNA sponges. Recent studies showed that many exonic circRNAs, generated by back-splicing of pre-mRNAs, can be translated in a cap-independent

    ...
    Antisense Oligo Pulldown of Circular RNA for Downstream Analysis
    用于下游分析的环状RNA的反义Oligo Pulldown
    作者:Debojyoti Das, Aniruddha Das and Amaresh C. Panda日期:07/20/2021,浏览量:733,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs (miRNAs) and RNA-binding proteins (RBPs). Several computational tools have been developed to predict circRNA interaction with target miRNAs and RBPs with a view to studying their potential effect

    ...
    RI-SEC-seq: Comprehensive Profiling of Nonvesicular Extracellular RNAs with Different Stabilities
    RI-SEC-seq:具有不同稳定性的非泡性细胞外RNA的综合分析
    作者:Juan Pablo Tosar, Fabiana Gámbaro, Mauricio Castellano and Alfonso Cayota日期:02/20/2021,浏览量:2420,Q&A: 0
    [Abstract]

    Exosomes and other extracellular vesicles (EVs) are considered the main vehicles transporting RNAs in extracellular samples, including human bodily fluids. However, a major proportion of extracellular RNAs (exRNAs) do not copurify with EVs and remain in ultracentrifugation supernatants of cell-conditioned medium or blood serum. We have observed

    ...
    Advances in Proximity Ligation in situ Hybridization (PLISH)
    PLISH技术的研究进展
    作者:Monica Nagendran, Adam M Andruska, Pehr B Harbury and Tushar J Desai日期:11/05/2020,浏览量:2453,Q&A: 0
    [Abstract] Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native in vivo spatial context. We developed a Proximity Ligation in situ Hybridization technology (PLISH) that enables quantitative measurement of single cell gene expression ...
    A CRISPR Competition Assay to Identify Cancer Genetic Dependencies
    基于CRISPR竞争法鉴定癌症的遗传依赖性
    作者:Vishruth Girish and Jason M. Sheltzer日期:07/20/2020,浏览量:4272,Q&A: 0
    [Abstract] The CRISPR/Cas9 system is a powerful tool for genome editing, wherein the RNA-guided nuclease Cas9 can be directed to introduce double-stranded breaks (DSBs) at a targeted locus. In mammalian cells, these DSBs are typically repaired through error-prone processes, resulting in insertions or deletions (indels) at the targeted locus. Researchers can ...
    Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis
    哺乳动物细胞脂筏的分离及其脂质组学分析
    作者:Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle and Dmitri Sviridov日期:07/05/2020,浏览量:2466,Q&A: 0
    [Abstract] Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and ...
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:7312,Q&A: 0
    [Abstract] Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development of single-molecule RNA fluorescence in situ hybridization (smFISH) allows the detection of ...
    Protocol for in situ Proximity Ligation Assay (PLA) and Microscopy Analysis of Epidermal Growth Factor Receptor (EGFR) Homodimerization
    原位邻位连接检测和显微镜分析表皮生长因子的同源二聚化的实验方法
    作者:Keiichi Ota and Taishi Harada日期:11/05/2018,浏览量:5446,Q&A: 0
    [Abstract] Oncogenic drivers play central roles in tumorigenesis as well as in tumor cell survival and proliferation. Mutations of the epidermal growth factor receptor gene (EGFR) that result in constitutive activation of the receptor tyrosine kinase have been identified as oncogenic drivers in a subset of non-small cell lung cancer (NSCLC). ...
    Microarray, IPA and GSEA Analysis in Mice Models
    关于小鼠模型的微阵列,IPA和GSEA分析
    作者:Stephanie N. Oprescu, Katharine A. Horzmann, Feng Yue, Jennifer L. Freeman and Shihuan Kuang日期:09/05/2018,浏览量:6935,Q&A: 0
    [Abstract] This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms underlying biological differences across two samples and thus can be applied to interrogate a variety ...
    Hypoxia Reporter Element Assay
    低氧反应元件的检测分析
    作者:Daelynn R Buelow and Sharyn D. D Baker日期:08/05/2018,浏览量:4507,Q&A: 0
    [Abstract] Hypoxia is a condition in which there is a decrease in oxygen supply to the cellular environment. Changes to cellular oxygen levels can lead to transcriptional changes of oxygen-regulated genes. Reporter assays are used to study gene expression alteration and modifications in response to environmental changes. Dual-reporter assays allow the ...