生物化学

分类

    现刊
    Protein Import Assay into Mitochondria Isolated from Human Cells
    从人细胞中分离的线粒体蛋白质输入测定
    作者:Lena M. Murschall, Esra Peker, Thomas MacVicar, Thomas Langer and Jan Riemer日期:06/20/2021,浏览量:1193,Q&A: 0
    [Abstract]

    Mitochondria are essential organelles containing approximately 1,500 proteins. Only approximately 1% of these proteins are synthesized inside mitochondria, whereas the remaining 99% are synthesized as precursors on cytosolic ribosomes and imported into the organelle. Various tools and techniques to analyze the import process have been developed.

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    Proteomics Profiling of S-sulfurated Proteins in Acinetobacter baumannii
    鲍曼不动杆菌硫酸化蛋白的蛋白质组学分析
    作者:Brenna J. C. Walsh and David P. Giedroc日期:05/05/2021,浏览量:1949,Q&A: 0
    [Abstract]

    Hydrogen sulfide (H2S) is emerging as an important modulator in bacterial cytoprotection against the host immune response in infected animals, which may well be attributed to downstream highly oxidized sulfur species, termed reactive sulfur species (RSS), derived from H2S. One mechanism by which H2S/RSS may signal in the cell is through proteome

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    Click Chemistry for Imaging in-situ Protein Palmitoylation during the Asexual Stages of Plasmodium falciparum
    恶性疟原虫无性期原位蛋白质棕榈酰化的点击化学成像
    作者:Mansoor A. Siddiqui日期:05/05/2021,浏览量:1910,Q&A: 0
    [Abstract]

    Palmitoylation refers to the modification of the cysteine thiols in proteins by fatty acids, most commonly palmitic acid, through ‘thioester bond’ formation. In vivo, palmitoylation of proteins is catalyzed by palmitoyl acyltransferases (PATs or DHHC-PATs). Palmitoylation has recently emerged as a crucial post-translational

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    In vitro Glutamylation Inhibition of Ubiquitin Modification and Phosphoribosyl-Ubiquitin Ligation Mediated by Legionella pneumophila Effectors
    嗜肺军团菌介导的泛素修饰和磷酸核糖泛素连接的体外谷氨酰化抑制
    作者:Alan G. Sulpizio, Marena E. Minelli and Yuxin Mao日期:11/05/2020,浏览量:982,Q&A: 0
    [Abstract]

    Glutamylation is a posttranslational modification where the amino group of a free glutamate amino acid is conjugated to the carboxyl group of a glutamate side chain within a target protein. SidJ is a Legionella kinase-like protein that has recently been identified to perform protein polyglutamylation of the Legionella SdeA Phosphoribosyl-Ubiquitin

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    Radioactive Assay of in vitro Glutamylation Activity of the Legionella pneumophila Effector Protein SidJ
    嗜肺军团菌效应蛋白SidJ体外谷氨酰化活性的放射性测定
    作者:Alan G. Sulpizio, Jung-Ho Shin, Marena E. Minelli and Yuxin Mao日期:10/05/2020,浏览量:1306,Q&A: 0
    [Abstract] The Legionella effector protein SidJ has recently been identified to perform polyglutamylation on another Legionella effector, SdeA, ablating SdeA’s activity. SidJ is a kinase-like protein that requires the small eukaryotic protein calmodulin to perform glutamylation. Glutamylation is a relatively uncommon type of ...
    A Workflow for Ultra-rapid Analysis of Histone Post-translational Modifications with Direct-injection Mass Spectrometry
    使用直接进样质谱对组蛋白翻译后修饰进行超快速分析的工作流程
    作者:Natarajan V. Bhanu, Simone Sidoli and Benjamin A Garcia日期:09/20/2020,浏览量:2526,Q&A: 0
    [Abstract] Chromatin modifications, like histone post translational modifications (PTMs), are critical for tuning gene expression and many other aspects of cell phenotype. Liquid chromatography coupled to mass spectrometry (LC-MS) has become the most suitable method to analyze histones and histone PTMs in a large-scale manner. Selected histone PTMs have ...
    Electrophoretic Mobility Shift Assay of in vitro Phosphorylated RNA Polymerase II Carboxyl-terminal Domain Substrates
    体外磷酸化的RNA聚合酶II羧基末端域底物的电泳迁移率测定
    作者:Joshua E. Mayfield, Seema Irani and Yan Zhang日期:06/20/2020,浏览量:2123,Q&A: 0
    [Abstract] Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit, RPB1, at specific residues. A variety of techniques ...
    In vitro Crosslinking Reactions and Substrate Incorporation Assays for The Identification of Transglutaminase-2 Protein Substrates
    转谷氨酰胺酶-2蛋白底物的体外交联反应和底物掺入鉴定
    作者:William L. Willis, Abigail Foster, Caitlin Henry, Lai Chu Wu and Wael Jarjour日期:06/20/2020,浏览量:1622,Q&A: 0
    [Abstract] Transglutaminase (TG2) catalyzes protein crosslinking between glutamyl and lysyl residues. Catalytic activity occurs via a transamidation mechanism resulting in the formation of isopeptide bonds. Since TG2-mediated transamidation is of mechanistic importance for a number of biological processes, assays that enable rapid and efficient ...
    Preparation, FPLC Purification and LC-FT-ICR-MS of Proteins
    蛋白质的制备、FPLC纯化及LC-FT-ICR-MS
    作者:Tyler B. Johnson, Jiri Adamec and Paul Blum日期:04/05/2020,浏览量:2231,Q&A: 0
    [Abstract] High magnetic field Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometers provide extremely high mass resolution (resolving power of ~200,000 at 400 m/z) protein detection across a broad mass range, enabling analysis of fine structure of isotopic peak clusters that is missed in other types of mass spectrometers. The protocol ...
    Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network
    反面高尔基体网状结构中货物蛋白包装成囊泡的无细胞重组
    作者:Xiao Tang, Feng Yang and Yusong Guo日期:03/05/2020,浏览量:2093,Q&A: 0
    [Abstract] Protein sorting at the trans Golgi network (TGN) plays important roles in targeting newly synthesized proteins to their specific destinations. The aim of this proposal is to reconstitute the packaging of non-Golgi resident cargo proteins into vesicles at the TGN, utilizing rat liver cytosol, semi-intact mammalian cells and nucleotides. ...