免疫学

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    现刊
    An Image-based Dynamic High-throughput Analysis of Adherent Cell Migration
    一种基于图像的动态高通量分析黏附细胞的迁移
    [Abstract]

    In this protocol, we describe a method to monitor cell migration by live-cell imaging of adherent cells. Scratching assay is a common method to investigate cell migration or wound healing capacity. However, achieving homogenous scratching, finding the optimal time window for end-point analysis and performing an objective image analysis imply, even

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    Multiplication and Growth Inhibition Activity Assays for the Zoonotic Malaria Parasite, Plasmodium knowlesi
    人畜共患病疟原虫——诺氏疟原虫增殖和生长抑制活性测定
    作者:Franziska Mohring, Thomas A. Rawlinson, Simon J. Draper and Robert W. Moon日期:09/05/2020,浏览量:2481,Q&A: 0
    [Abstract] Malaria remains a major cause of morbidity and mortality globally. Clinical symptoms of the disease arise from the growth and multiplication of Plasmodium parasites within the blood of the host. Thus in vitro assays to determine how drug, antibody and genetic perturbations affect the growth rate of Plasmodium parasites ...
    A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
    类风湿性关节炎患者单个B细胞中瓜氨酸特异单克隆抗体的改进鉴定方案
    [Abstract] We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from single B cells of your choice and followed by amplification of the variable region of immunoglobulin ...
    Nab Escaping AAV Mutants Isolated from Mouse Muscles
    从小鼠肌肉中分离Nab逃脱AAV突变体
    作者:Zheng Chai, R. Jude Samulski and Chengwen Li日期:05/05/2018,浏览量:4765,Q&A: 0
    [Abstract] Neutralizing antibodies (Nabs) are a major challenge in clinical trials of adeno-associated virus (AAV) vector gene therapy, because Nabs are able to inhibit AAV transduction in patients. We have successfully isolated several novel Nab-escaped AAV chimeric capsids in mice by administrating a mixture of AAV shuffled library and patient serum. These ...
    Hemagglutination Inhibition (HI) Assay of Influenza Viruses with Monoclonal Antibodies
    单克隆抗体介导的流感病毒血细胞凝集抑制(HI)试验
    [Abstract] Heamagglutination is inhibited when antibodies are present because antibodies to the influenza virus will prevent attachment of the virus to red blood cells. The highest dilution of antibody that prevents hemagglutination is called the HI titer. Human monoclonal antibodies generated from single human B cells were tested to characterize their ...
    Micro Neutralization (MN) Assay of Influenza Viruses with Monoclonal Antibodies
    单克隆抗体介导的流感病毒微中和(MN)测定试验
    [Abstract] The human monoclonal antibodies generated from single human B cells were tested to characterize their ability to neutralize virus infectivity. The microneutralization assay is a highly sensitive and specific assay for detecting virus-specific neutralizing antibodies to influenza viruses. This protocol is to measure the ability of human monoclonal ...
    Cell-based Assays to Monitor AID Activity
    基于细胞的监测AID活性的方法
    作者:Ludivine C. Litzler, Stephen P. Methot, Anne-Marie Patenaude, Astrid Zahn and Javier M. Di Noia日期:02/05/2016,浏览量:7189,Q&A: 0
    [Abstract] The enzyme Activation induced deaminase (AID) underpins antibody affinity maturation and isotype switching through its mutagenic activity of deaminating deoxycytidine to deoxyuridine in DNA. Subsequent processing of the deoxyuridine initiates the processes of somatic hypermutation (SHM) and class switch recombination (CSR) in B cells. ...
    Binding Affinity Measurement of Antibodies from Crude Hybridoma Samples by SPR
    表面等离子共振法测定原初杂交瘤样本中抗体与抗原的结合亲和力
    [Abstract] Surface Plasmon Resonance (SPR) is widely used to generate kinetic and affinity information on specific interactions between biomolecules. This technique is label-free and monitors the binding event in real-time. It is generally used for characterization of monoclonal antibody - antigen interactions. This protocol describes specifically the use of ...
    Detection of Tumor Cell Surface-reactive Antibodies
    肿瘤细胞膜结合性抗体的检测
    作者:Brian M. Andersen, Michelle R. Goulart, Michael R. Olin and G. Elizabeth Pluhar日期:05/05/2014,浏览量:6927,Q&A: 0
    [Abstract] Vaccine-based immunotherapy is being used to treat dogs with primary brain tumors. The vaccines are composed of a lysate of autologous tumor cells, which stimulate an immune response producing tumor specific antibodies that are capable of inducing antibody-dependent cell-mediated cytotoxicity to allogeneic, as well as autologous, tumor cells. This ...
    DNA PCR Assays for Igh Rearrangement
    基于DNA的PCR法分析免疫球蛋白重链(Igh)的重排
    作者:Yusuke Satoh, Takao Sudo and Takafumi Yokota日期:02/20/2014,浏览量:8562,Q&A: 0
    [Abstract] This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].