分子生物学

分类

    现刊
    Efficient and Rapid Analysis of Polysomes and Ribosomal Subunits in Cells and Tissues Using Ribo Mega-SEC
    利用Ribo Mega-SEC高效快速分析细胞和组织中的多聚体和核糖体亚基
    作者:Harunori Yoshikawa, Ramasubramanian Sundaramoorthy, Daniel Mariyappa, Hao Jiang and Angus I. Lamond日期:08/05/2021,浏览量:1243,Q&A: 0
    [Abstract]

    Polysome profile analysis is a popular method for separating polysomes and ribosomal subunits and is typically achieved using a sucrose density gradient (SDG). This has remained the gold standard method since ribosomes were first discovered; however, this method is time-consuming and requires multiple steps from making the gradient and long

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    Antisense Oligo Pulldown of Circular RNA for Downstream Analysis
    用于下游分析的环状RNA的反义Oligo Pulldown
    作者:Debojyoti Das, Aniruddha Das and Amaresh C. Panda日期:07/20/2021,浏览量:742,Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs (miRNAs) and RNA-binding proteins (RBPs). Several computational tools have been developed to predict circRNA interaction with target miRNAs and RBPs with a view to studying their potential effect

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    A New Method for Studying RNA-binding Proteins on Specific RNAs
    一种研究特异性RNA结合蛋白的新方法
    作者:Weiping Sun, Ziheng Zhang, Ji-Long Liu and Min Zhuang日期:05/20/2021,浏览量:6360,Q&A: 0
    [Abstract]

    Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by combining the RNA-binding capacity of CRISPR/Cas13 and the proximity-tagging activity of PUP-IT.

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    Biochemical Pulldown of mRNAs and Long Noncoding RNAs from Cellular Lysates Coupled with Mass Spectrometry to Identify Protein Binding Partners
    通过细胞裂解液中mRNAs和长链非编码RNA的生化沉降结合质谱法鉴定蛋白结合物
    作者:Anca F. Savulescu, Stoyan Stoychev, Sipho Mamputha and Musa M. Mhlanga日期:06/05/2020,浏览量:2900,Q&A: 2
    [Abstract] RNA binding proteins (RBPs) interact with cellular mRNAs, controlling various steps throughout the lifetime of these transcripts, including transcription, cellular transport, subcellular localization, translation and degradation. In addition to binding mRNA transcripts, a growing number of RBPs are shown to bind long noncoding RNAs (lncRNAs), ...
    Identification of RNase-sensitive LINE-1 Ribonucleoprotein Interactions by Differential Affinity Immobilization
    利用差异化亲和固定鉴定核糖核酸酶敏感的LINE-1核糖核蛋白间的相互作用
    作者:Hua Jiang, Martin S. Taylor, Kelly R. Molloy, Ilya Altukhov and John LaCava日期:04/05/2019,浏览量:4528,Q&A: 0
    [Abstract] Long Interspersed Nuclear Element-1 (LINE-1, L1) constitutes a family of autonomous, self-replicating genetic elements known as retrotransposons. Although most are inactive, copious L1 sequences populate the human genome. L1s proliferate in a ‘copy-and-paste’ fashion through an RNA intermediate; a full-length L1 transcript is ~6,000 nucleotides ...
    RNA Immunoprecipitation Assay to Determine the Specificity of SRSF3 Binding to Nanog mRNA
    利用RNA免疫沉淀试验确定SRSF3结合Nanog mRNA的特异性
    作者:Madara Ratnadiwakara and Minna-Liisa Änko日期:11/05/2018,浏览量:5454,Q&A: 0
    [Abstract] Mammalian cells express hundreds of RNA binding proteins (RBPs) that are essential regulators of RNA metabolism. RBP activity plays a central role in the control of gene expression programs and identification of RNA-protein interactions is critical for comprehensive understanding of gene regulation in cells. In recent years, various tools and ...
    Detecting the Interaction of Double-stranded RNA Binding Protein, Viral Protein and Primary miRNA Transcript by Co-immunoprecipitation in planta
    通过免疫共沉淀法检测植物中双链RNA结合蛋白、病毒蛋白和初级miRNA转录产物的交互作用
    作者:Lijia Zheng, Chao Zhang, Jianguo Wu and Yi Li日期:05/05/2018,浏览量:4068,Q&A: 0
    [Abstract] MicroRNAs (miRNAs) play important roles in plant growth, development, and response to infection by microbes. Double-stranded RNA binding protein 1 (DRB1) facilitates the processing of primary miRNA transcripts into mature miRNAs. Recently, we found that NS3 protein encoded by rice stripe virus (RSV) associates with DRB1 and promotes miRNA ...
    Trimolecular Fluorescence Complementation (TriFC) Assay for Direct Visualization of RNA-Protein Interaction in planta
    三分子荧光互补(TriFC)实验直接观察植物中RNA-蛋白质的相互作用
    作者:Jun Sung Seo and Nam-Hai Chua日期:10/20/2017,浏览量:8436,Q&A: 0
    [Abstract] RNA-Protein interactions play important roles in various eukaryotic biological processes. Molecular imaging of subcellular localization of RNA/protein complexes in plants is critical for understanding these interactions. However, methods to image RNA-Protein interactions in living plants have not yet been developed until now. Recently, we have ...
    RNA-protein UV-crosslinking Assay
    紫外交联实验检测RNA-蛋白的相互作用
    作者:Dipak Kumar Poria and Partho Sarothi Ray日期:03/20/2017,浏览量:13459,Q&A: 2
    [Abstract] RNA-protein interactions play a crucial role in every aspect of RNA metabolism, and also plays a major role in post-transcriptional gene regulation. RNA-binding proteins have been implicated in viral gene expression (Ray and Das, 2002) and microRNA-mediated gene regulation (Poria et al., 2016). Here we have described the protocol which ...
    Polysome Fractionation to Analyze mRNA Distribution Profiles
    多聚核糖体分馏分析mRNA分布特征
    作者:Amaresh C. Panda, Jennifer L. Martindale and Myriam Gorospe日期:02/05/2017,浏览量:22989,Q&A: 5
    [Abstract] Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that ...