干细胞

分类

    现刊
    A Modified Semisolid Clonal Culture for Identification of B-1 and B-2 Progenitor Colony Forming Ability of Mouse Embryonic Hemogenic Endothelial Cells
    改良半固态克隆培养鉴定小鼠胚胎造血内皮细胞B-1和B-2祖细胞集落形成能力
    作者:Michihiro Kobayashi and Momoko Yoshimoto日期:05/05/2020,浏览量:1632,Q&A: 0
    [Abstract] The search for the origin of the first hematopoietic stem cells (HSCs) in the mouse embryo has been a hot topic in the field of developmental hematopoiesis. Detecting lymphoid potential is one of the supportive evidence to show the definitive hematopoietic activity of HSCs. However, the first B-lymphoid potential in the mouse embryos are reported ...
    Quantification of Mouse Hematopoietic Progenitors’ Formation Using Time-lapse Microscopy and Image Analysis
    使用延时显微技术和图像分析定量小鼠造血祖细胞的形成
    作者:Isabelle Bergiers, Christian Tischer, Özge Vargel Bölükbaşı and Christophe Lancrin日期:01/05/2019,浏览量:3983,Q&A: 0
    [Abstract] In vitro differentiation of mouse embryonic stem cells (mESCs) towards blood cells constitutes a well-established system to study the endothelial-to-hematopoietic transition (EHT) at the onset of blood development. Assessing the emergence of small non-adherent round blood cells in the culture without disturbing it is essential to evaluate ...
    FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
    基于FACS利用EGF配体从新鲜人组织中分离神经和胶质瘤干细胞群
    作者:Jessica Tome-Garcia, Fiona Doetsch and Nadejda M. Tsankova日期:12/20/2017,浏览量:9610,Q&A: 0
    [Abstract] Direct isolation of human neural and glioma stem cells from fresh tissues permits their biological study without prior culture and may capture novel aspects of their molecular phenotype in their native state. Recently, we demonstrated the ability to prospectively isolate stem cell populations from fresh human germinal matrix and glioblastoma ...
    Single-cell Visualization of Chromosome Transcriptional Territories by RNA-paint
    通过RNA染色显示单细胞的染色体转录区
    作者:Céline Vallot and Claire Rougeulle日期:09/05/2016,浏览量:7216,Q&A: 0
    [Abstract] We developed a FISH-based method to directly assess chromosome-wide transcriptional activity, thereby enabling the visualization of the actively transcribed fraction of a chromosome at the single-cell level. We applied this method to probe the activity of X-chromosomes and its instability in the context of human embryonic stem cells and cancer ...
    Single-cell Gene Expression Profiling of Mouse Stem Cells With Fluidigm BiomarkTM Dynamic Array
    采用Fluidigm Biomark™动态芯片检测小鼠干细胞中的单细胞基因表达谱
    作者:Ana Sevilla日期:05/05/2013,浏览量:16450,Q&A: 3
    [Abstract] This protocol describes how to use Fluidigm BiomarkTM 96.96 dynamic arrays for high-throughput expression profiling from single mouse stem cells, assaying up to 96 independent samples with up to 96 quantitative PCR (qPCR) probes (equivalent to 9,216 reactions) in a single experiment. This Dynamic Array contains a network of microfluidic ...
    Synchronize Human Embryonic Stem Cells at Different Cell Cycle Stage
    人类胚胎干细胞在细胞分裂周期不同阶段的同步实验
    作者:Hui Zhu日期:06/05/2012,浏览量:18932,Q&A: 0
    [Abstract] Pluripotency and the capability for self-renewal are essential characteristics of human embryonic stem cells (hESCs), which hold great potential as a cellular source for tissue replacement. Short cell cycle (15-16 h) compared to somatic cells is another property of hESCs. Efficient synchronization of hESCs at different cell cycle stages is ...