植物科学

分类

    现刊
    Phytophthora infestans (Late blight) Infection Assay in a Detached Leaf of Potato
    在离体叶片中的马铃薯疫霉(晚疫病)感染实验
    作者:Hari S. Karki and Dennis A. Halterman日期:02/20/2021,浏览量:3062,Q&A: 0
    [Abstract]

    Phytophthora infestans is a hemibiotroph oomycete that primarily infects potato and tomato. It infects stems, leaves, and tubers and fruits of potato and tomato. High throughput and reproducible infection assays are prerequisites to find sources of resistance in any crop. In this protocol, we describe a detached leaf assay (DLA) for conducting the

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    Rice Ragged Stunt Virus Propagation and Infection on Rice Plants
    稻株中水稻齿叶矮缩病毒的传播和感染
    作者:Chao Zhang, Chaonan Shi, Dong Chen and Jianguo Wu日期:10/20/2018,浏览量:3734,Q&A: 0
    [Abstract] Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice Ragged Stunt Virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH), can suppress jasmonic acid-mediated antiviral defense through ...
    Fusarium graminearum Inoculation on Wheat Head
    禾谷镰刀菌的麦穗接种
    作者:Chanjing Feng, Huiquan Liu and Zhe Tang日期:08/05/2018,浏览量:5828,Q&A: 0
    [Abstract] Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis mechanisms and breeding for disease resistance plants. The efficiency of these actions could be evaluated by ...
    Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
    水稻幼苗RYMV接种和抗性评估方法
    作者:Agnès Pinel-Galzi, Eugénie Hébrard, Oumar Traoré, Drissa Silué and Laurence Albar日期:06/05/2018,浏览量:5084,Q&A: 0
    [Abstract] Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and ...
    In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato
    番茄离体及活体灰葡萄孢菌接种试验
    作者:Jiajie Lian, Hongyu Han, Jiuhai Zhao and Chuanyou Li日期:04/20/2018,浏览量:9833,Q&A: 0
    [Abstract] Botrytis cinerea (B. cinerea) attacks many crops of economic importance, represents one of the most extensively studied necrotrophic pathogens. Inoculation of B. cinerea and phenotypic analysis of plant resistance are key procedures to investigate the mechanism of plant immunity. Here we describe a protocol for B. ...
    Large Scale Field Inoculation and Scoring of Maize Southern Leaf Blight and Other Maize Foliar Fungal Diseases
    玉米南方叶斑病及其他叶片真菌病的大面积野外接种与评分
    作者:Shannon M. Sermons and Peter J. Balint-Kurti日期:03/05/2018,浏览量:6449,Q&A: 0
    [Abstract] Field-grown maize is inoculated with Cochliobolus heterostrophus, causal agent of southern leaf blight disease, by dropping sorghum grains infested with the fungus into the whorl of each maize plant at an early stage of growth. The initial lesions produce secondary inoculum that is dispersed by wind and rain, causing multiple cycles of ...
    Quantification of Plant Cell Death by Electrolyte Leakage Assay
    通过电解质渗漏测定法定量植物细胞死亡
    作者:Noriyuki Hatsugai and Fumiaki Katagiri日期:03/05/2018,浏览量:17653,Q&A: 2
    [Abstract] We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is designed to measure the electrolyte leakage from a tissue sample over a time course, so that the extent of cell death in the tissue can be monitored ...
    Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method
    通过剪叶法接种水稻黄单胞菌水稻致病变种并测定其生长速率
    作者:Yinggen Ke, Shugang Hui and Meng Yuan日期:10/05/2017,浏览量:13154,Q&A: 0
    [Abstract] Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of Xoo and disease scoring are necessary to investigate the nature of the disease and the mechanism of plant ...
    Wheat Coleoptile Inoculation by Fusarium graminearum for Large-scale Phenotypic Analysis
    用于大规模表型分析的小麦胚芽鞘禾谷镰孢菌接种
    作者:Lei-Jie Jia, Wan-Qiu Wang and Wei-Hua Tang日期:08/05/2017,浏览量:8326,Q&A: 0
    [Abstract] The ascomycete fungus Fusarium graminearum is a destructive fungal pathogen of wheat, barley and maize. Although reverse genetics and homologous recombination gene deletion methods have generated thousands of gene deletion mutants of F. graminearum, evaluating virulence of these fungal mutants is still a rate-limiting step. Here ...
    Isolation of Ustilago bromivora Strains from Infected Spikelets through Spore Recovery and Germination
    通过孢子恢复和萌发从感染的小穗分离雀麦黑粉菌菌株
    作者:Jason Bosch and Armin Djamei日期:07/20/2017,浏览量:5991,Q&A: 0
    [Abstract] Ustilago bromivora is a biotrophic smut fungus infecting Brachypodium sp. It is closely related to the barley-infecting smut Ustilago hordei, and related to the well-studied, gall-inducing model pathogen Ustilago maydis. Upon flowering, the spikelets of U. bromivora-infected plants are filled with black ...