细胞生物学

分类

    现刊
    Cryo-transmission Electron Microscopy of Outer-inner Membrane Vesicles Naturally Secreted by Gram-negative Pathogenic Bacteria
    革兰阴性致病菌自然分泌的外-内膜囊泡低温透射电子显微镜观察
    作者:Lidia Delgado, Nicolás Baeza, Carla Pérez-Cruz, Carmen López-Iglesias and Elena Mercadé日期:09/20/2019,浏览量:3232,Q&A: 0
    [Abstract] A protocol was developed to visualize and analyze the structure of membrane vesicles (MVs) from Gram-negative bacteria. It is now accepted that these micrometric spherical vesicles are commonly produced by cells from all three domains of life, so the protocol could be useful in the study of vesicles produced by eukaryotes and archaea as well as ...
    Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs
    通过电镜样品制备实现的人体组织和器官中细胞连接体及其定位的纳米与介观图谱分析
    作者:Lucy Ngo, Anton D. Nathanson, Tomasz Garbowski, Ulf Knothe, Dirk Zeidler and Melissa L. Knothe Tate日期:07/20/2019,浏览量:4777,Q&A: 0
    [Abstract] Multibeam scanning electron microscopy (multiSEM) provides a technical platform for seamless nano-to-mesoscale mapping of cells in human tissues and organs, which is a major new initiative of the U.S. National Institutes of Health. Such cross-length-scale imaging is expected to provide unprecedented understanding of relationships between cellular ...
    Preparation and Manipulation of Olfactory Epithelium Explant Cultures for Measurement of the Mechanical Tension of Individual Axons Using the Biomembrane Force Probe
    嗅上皮外植体的制备及培养以用于通过生物膜力学探针测定单个轴突的机械张力
    作者:Coralie Fouquet and Alain Trembleau日期:04/20/2019,浏览量:3139,Q&A: 0
    [Abstract] In this paper, we describe a protocol allowing measurement of the mechanical tension of individual axons grown ex vivo from neural tissue explants. This protocol was developed with primary cultures of olfactory epithelium explants from embryonic (E13.5) mice. It includes a detailed description of explant dissection and culture, as well as ...
    Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy
    利用电子显微镜免疫金标记法进行植物细胞壁表位的可视化观察
    作者:Mateusz Majda日期:04/05/2019,浏览量:4352,Q&A: 0
    [Abstract] Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane. The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development. The visualization of cell wall components in ...
    Electron Microscopic Detection of Proteins and Protein Complexes in Mammalian Cells Using APEX-tagged, Conditionally Stable Nanobodies
    利用APEX标记的条件性稳定纳米体电子显微检测哺乳动物细胞中的蛋白和蛋白复合物
    作者:Thomas E. Hall, James Rae, Nicholas Ariotti and Robert G. Parton日期:11/20/2018,浏览量:3752,Q&A: 0
    [Abstract] The recent development of 3D electron microscopic techniques for cells and tissues has necessitated the development of new methods for the detection of proteins and protein-complexes in situ. The development of new genetic tags, such as the ascorbate peroxidase, APEX, for electron microscopic detection of tagged proteins has expanded the ...
    Examining Autophagy in Plant by Transmission Electron Microscopy (TEM)
    利用透射电镜检测植物细胞自噬
    作者:Xiyin Zheng, Chenguang Zhao and Yule Liu日期:10/20/2018,浏览量:4202,Q&A: 0
    [Abstract] In plants, macroautophagy, here referred as autophagy, is a degradation pathway during which the double-membrane structure named autophagosome engulfs the cargo and then fuses with vacuole for material recycling.

    To investigate the process of autophagy, transmission electron microscopy (TEM) was used to monitor the ultrastructure of ...
    Metal-tagging Transmission Electron Microscopy for Localisation of Tombusvirus Replication Compartments in Yeast
    使用金属标签透射电子显微技术定位酵母菌中番茄丛矮病毒复制区
    作者:Isabel Fernández de Castro and Cristina Risco日期:04/20/2018,浏览量:4837,Q&A: 0
    [Abstract] Positive-stranded (+) RNA viruses are intracellular pathogens in humans, animals and plants. To build viral replicase complexes (VRCs) viruses manipulate lipid flows and reorganize subcellular membranes. Redesigned membranes concentrate viral and host factors and create an environment that facilitates the formation of VRCs within replication ...
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:6999,Q&A: 0
    [Abstract] Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various proteins. Unfortunately, they are also hijacked by phyto-viruses, enabling them to spread from ...
    Immunogold Electron Microscopy of the Autophagosome Marker LC3
    自噬体标志物LC3的免疫金电镜观察
    作者:Domenico Mattoscio, Andrea Raimondi, Carlo Tacchetti and Susanna Chiocca日期:12/20/2017,浏览量:9894,Q&A: 0
    [Abstract] Even though autophagy was firstly observed by transmission electron microscopy already in the 1950s (reviewed in Eskelinen et al., 2011), nowadays this technique remains one of the most powerful systems to monitor autophagic processes. The autophagosome, an LC3-positive double membrane structures enclosing cellular materials, represents ...
    Using Light and Electron Microscopy to Estimate Structural Variation in Thylakoid Membranes
    使用光学和电子显微镜估计类囊体膜的结构变异
    作者:Hatsumi Nozue and Kiyokazu Kametani日期:12/05/2017,浏览量:7247,Q&A: 0
    [Abstract] The shapes of chloroplasts and the architectures of internal thylakoid membranes are altered by growth and environmental changes (Lichtenthaler et al., 1981; Kutik, 1985; Terashima and Hikosaka, 1995). These morphological alterations proceed via transitional intermediates, during which dynamic and heterogeneous thylakoid membranes are ...