细胞生物学

分类

    现刊
    A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
    一个健康的乳腺有机样系统来模拟哺乳和退化样过程
    作者:Elsa Charifou, Jakub Sumbal, Zuzana Koledova, Han Li and Aurélie Chiche日期:04/20/2021,浏览量:2658,Q&A: 0
    [Abstract]

    The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding

    ...
    Using the Parafilm-assisted Microdissection (PAM) Method to Sample Rodent Nucleus Accumbens
    应用膜辅助显微切割(PAM)对啮齿类伏隔核进行采样
    [Abstract] Microdissection techniques are very important for anatomical and functional studies focused on neuroscience, where it is often necessary microdissect specific brain areas to perform molecular or anatomical analyses. The parafilm®-assisted microdissection (PAM) was previously described and involves the microdissection of tissue ...
    Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands
    小鼠乳腺单个上皮细胞悬液制备
    作者:Heng Sun, Xiaoling Xu and Chuxia Deng日期:02/20/2020,浏览量:2506,Q&A: 0
    [Abstract] Single cell RNA sequencing is a very powerful means for cellular heterogeneous studies and so becoming wildly utilized nowadays. To guarantee the success of such analysis, it is very important, though sometimes difficult, to obtain single cells suspension with high quality, especially from the primary solid organs like mammary glands. Digestion of ...
    Acute Cerebellar Slice Preparation Using a Tissue Chopper
    使用组织切碎机快速制备小脑切片
    作者:Yubin Wang and Michel Baudry日期:03/05/2019,浏览量:3673,Q&A: 0
    [Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
    Identification and Quantitation of Leukocyte Populations in Human Kidney Tissue by Multi-parameter Flow Cytometry
    利用多参数流式细胞技术对人肾脏组织中白细胞种群的鉴定与定量分析
    作者:Katrina Kildey, Becker M.P. Law, Kimberly A. Muczynski, Ray Wilkinson, Helen Healy and Andrew J. Kassianos日期:08/20/2018,浏览量:6226,Q&A: 0
    [Abstract] Inflammatory immune cells play direct pathological roles in cases of acute kidney injury (AKI) and chronic kidney disease (CKD). However, the identification and characterization of distinct populations of leukocytes in human kidney biopsies have been confounded by the limitations of immunohistochemical (IHC)-based techniques used to detect them. ...
    In vitro Explant Cultures to Interrogate Signaling Pathways that Regulate Mouse Lung Development
    探寻调控小鼠肺部发育的信号转导途径的体外外植体培养
    作者:Changfu Yao, Gianni Carraro and Barry R. Stripp日期:05/20/2018,浏览量:5058,Q&A: 0
    [Abstract] Early mouse lung development, including specification of primordia, patterning of early endoderm and determination of regional progenitor cell fates, is tightly regulated. The ability to culture explanted embryonic lung tissue provides a tractable model to study cellular interactions and paracrine factors that regulate these processes. We provide ...
    Organotypic Explants of the Embryonic Rodent Hippocampus: An Accessible System for Transgenesis
    啮齿动物胚胎海马器官型外植体:一个可进行转基因的系统
    作者:Archana Iyer , Lakshmi Subramanian and Shubha Tole日期:03/20/2018,浏览量:5189,Q&A: 0
    [Abstract] This protocol describes the technique of ex-vivo electroporation to target embryonic hippocampal progenitors in an organotypic slice preparation. This technique allows gene perturbation for examining developmental processes in the embryonic hippocampus while retaining the environment and connectivity of the cells. Gene perturbation can ...
    Obtaining Acute Brain Slices
    急性脑组织切片的制备
    作者:Thomas Papouin and Philip G. Haydon日期:01/20/2018,浏览量:16955,Q&A: 1
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
    Ex vivo Trophoblast-specific Genetic Manipulation Using Lentiviral Delivery
    使用慢病毒递送的离体滋养层特定基因操作
    作者:Damayanti Chakraborty, Masanaga Muto and Michael J. Soares日期:12/20/2017,浏览量:5561,Q&A: 0
    [Abstract] In this protocol report, we describe a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Lentiviral packaged gene constructs can be efficiently and specifically delivered to the trophoblast cell lineage of the blastocyst. The consequences of ‘gain-of-function’ and ‘loss-of-function’ blastocyst ...
    Mandibular Explant Assay for Investigating Extrinsic Stimuli on Bone and Cartilage Development
    研究骨及软骨外源刺激的下颌外植体实验
    作者:Jamie Jiang, Jessica W. Bertol and Walid D. Fakhouri日期:12/05/2017,浏览量:5621,Q&A: 0
    [Abstract] A major issue in developmental biology is to determine how embryonic tissues respond to molecular signals in a timely manner and given the position-restricted instructions during morphogenesis, of which Meckel’s cartilage is a classical example. The ex-vivo explant model is a practical and convenient system that allows investigation of ...