微生物学

分类

    现刊
    Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages
    两个不同植物谱系根内真菌结构的酸化蓝墨水染色法观察流程
    作者:Jill Kowal, Elena Arrigoni and Sophie Lane日期:10/20/2020,浏览量:1321,Q&A: 0
    [Abstract] Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure. By modifying established protocols, our root staining method stains roots using a safe ink- and ...
    Method for Assessing Virulence of Colletotrichum higginsianum on Arabidopsis thaliana Leaves Using Automated Lesion Area Detection and Measurement
    应用损伤面积的自动化检测和测量评价炭疽菌对拟南芥叶片致病性的方法
    作者:Ayako Tsushima, Pamela Gan and Ken Shirasu日期:11/20/2019,浏览量:3595,Q&A: 0
    [Abstract] The plant pathogenic fungus, Colletotrichum higginsianum is widely used to understand infection mechanisms, as it infects the model plant Arabidopsis thaliana. To determine the virulence of C. higginsianum, several methods have been developed, such as disease reaction scoring, lesion measurement, entry rate assays, and ...
    Endocytosis Detection in Magnaporthe oryzae
    稻瘟病菌内吞作用的检测
    作者:Muxing Liu and Zhengguang Zhang日期:08/05/2019,浏览量:2293,Q&A: 0
    [Abstract] Endocytosis is an intracellular trafficking pathway that occurs in nutrient uptake, signal transduction and reconstruction of cell polarity and is conserved in eukaryotic cells. In fungi, endocytosis plays crucial roles in the physiology of hyphal growth and pathogenicity. vidence for endocytosis in filamentous fungi is detected by the ...
    Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
    蓝甲醇衍生物作为丛枝菌根真菌与植物共生标志物的定量研究
    作者:Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin日期:07/20/2019,浏览量:3517,Q&A: 0
    [Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
    Isolation of Powdery Mildew Haustoria from Infected Barley
    感染大麦中白粉病吸器的分离提取
    作者:Linhan Li, Benjamin Collier and Pietro D. Spanu日期:07/20/2019,浏览量:4254,Q&A: 0
    [Abstract] Blumeria graminis is a fungus that causes powdery mildews on grasses, such as barley. Investigations of this pathogen present many challenges due to its obligate biotrophic nature. This means that the fungus can only grow in the presence of a living host plant. B. graminis forms epiphytic mycelia on the plant surface and feeding ...
    Field Inoculation and Classification of Maize Ear Rot Caused by Fusarium verticillioides
    田间接种和轮枝镰孢菌引起的玉米穗粒腐病的分类
    作者:Chaopei Dong, Yabin Wu, Jingyang Gao, Zijian Zhou, Cong Mu, Peipei Ma, Jiafa Chen and Jianyu Wu日期:12/05/2018,浏览量:4301,Q&A: 0
    [Abstract] Maize ear rot is a worldwide fungal disease mainly caused by Fusarium verticillioides and Fusarium graminearum. Maize planted in the field was inoculated with Fusarium verticillioides at the filling stage, 15 days after pollination. Two milliliters of spore suspension with a concentration of 5 x 106/ml was ...
    Preparation and Purification of Proteins Secreted from Phytophthora sojae
    大豆疫霉菌分泌蛋白的制备和纯化
    作者:Yeqiang Xia, Yan Wang and Yuanchao Wang日期:10/20/2018,浏览量:4140,Q&A: 1
    [Abstract] Phytophthora sojae, the causal agent of soybean root and stem rot, is responsible for enormous economic losses in soybean production. P. sojae secrets various effectors to reprogram host immunity. The plant apoplastic space is a major battleground in plant-pathogen interactions. Here we describe a protocol for purification and ...
    Artificial Inoculation of Epichloë festucae into Lolium perenne, and Visualisation of Endophytic and Epiphyllous Fungal Growth
    人工接种羊茅香柱菌到麦黑草,并可视化观察内生真菌和叶生真菌的生长
    作者:Yvonne Becker, Kimberly A. Green, Barry Scott and Matthias Becker日期:09/05/2018,浏览量:5516,Q&A: 0
    [Abstract] Natural hosts for the fungal endophyte Epichloë festucae include Festuca rubra (fine fescue) and Festuca trachyphylla (hard fescue). Some strains also form stable associations with Lolium perenne (perennial ryegrass). L. perenne is a suitable host to study fungal endophyte–grass interactions, such as endophytic ...
    In vivo and in vitro 31P-NMR Study of the Phosphate Transport and Polyphosphate Metabolism in Hebeloma cylindrosporum in Response to Plant Roots Signals
    利用31P-NMR技术体内外研究柱孢白桦在响应植物根组织信号后的磷酸盐转运和多聚磷酸盐代谢
    [Abstract] We used in vivo and in vitro phosphorus-31 nuclear magnetic resonance (31P-NMR) spectroscopy to follow the change in transport, compartmentation and metabolism of phosphate in the ectomycorrhizal fungus Hebeloma cylindrosporum in response to root signals originating from host (Pinus pinaster) or ...
    Fusarium graminearum Inoculation on Wheat Head
    禾谷镰刀菌的麦穗接种
    作者:Chanjing Feng, Huiquan Liu and Zhe Tang日期:08/05/2018,浏览量:5676,Q&A: 0
    [Abstract] Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis mechanisms and breeding for disease resistance plants. The efficiency of these actions could be evaluated by ...