细胞生物学

分类

    现刊
    Analysis of the Effects of Hexokinase 2 Detachment From Mitochondria-Associated Membranes with the Highly Selective Peptide HK2pep
    用高选择性肽HK2pep分析己糖激酶2脱离线粒体相关膜的作用
    作者:Francesco Ciscato, Federica Chiara, Riccardo Filadi and Andrea Rasola日期:07/20/2021,浏览量:1217,Q&A: 0
    [Abstract]

    The crucial role of hexokinase 2 (HK2) in the metabolic rewiring of tumors is now well established, which makes it a suitable target for the design of novel therapies. However, hexokinase activity is central to glucose utilization in all tissues; thus, enzymatic inhibition of HK2 can induce severe adverse effects. In an effort to find a selective

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    Evaluation of Toxicity with Brine Shrimp Assay
    通过卤虫实验进行毒性评价
    作者:Christina N. Banti and Sotiris K. Hadjikakou日期:01/20/2021,浏览量:2574,Q&A: 0
    [Abstract]

    The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with

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    Photodynamic Therapy in a 3D Model of Ovarian Cancer
    卵巢癌3D模型中的光动力学治疗
    作者:Shubhankar Nath and Kaitlin Moore日期:08/05/2019,浏览量:3055,Q&A: 0
    [Abstract] Photodynamic therapy (PDT), is a clinically-approved light-based anti-cancer treatment modality in which a photoactivatable photosensitizer is irradiated with an appropriate wavelength of light to generate cytotoxic molecules to kill cancer cells. In this article, we describe an in vitro PDT protocol using a 3-dimensional (3D) model of ...
    Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
    蚀斑测定弗氏志贺在TC7人肠上皮细胞中的侵袭和细胞间传播
    作者:Atin Sharma and Andrea Puhar日期:07/05/2019,浏览量:3148,Q&A: 0
    [Abstract] Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to gain access to the cytosol. Thereafter Shigella disseminates to neighboring cells in an ...
    Isolation of Neural Stem Cells from the Embryonic Mouse Hippocampus for in vitro Growth or Engraftment into a Host Tissue
    从胚胎小鼠海马分离神经干细胞用于体外生长或植入宿主组织
    作者:Oksana Rybachuk, Olga Kopach, Tetyana Pivneva and Vitaliy Kyryk日期:02/20/2019,浏览量:5649,Q&A: 0
    [Abstract] For both stem cell research and treatment of the central nervous system disorders, neural stem/progenitor cells (NSPCs) represent an important breakthrough tool. In the expanded stem cell-based therapy use, NSPCs not only provide a powerful cell source for neural cell replacement but a useful model for developmental biology research. Despite ...
    Cell Microencapsulation and Cryopreservation with Low Molecular Weight Hyaluronan and Dimethyl Sulfoxide
    利用低分子量透明质酸和二甲基亚砜进行细胞微胶囊化和冷冻保存
    作者:H. Gurruchaga, L. Saenz del Burgo, G. Orive, R. M. Hernandez, J. Ciriza and J. L. Pedraz日期:02/20/2019,浏览量:3605,Q&A: 0
    [Abstract] Cryopreservation is commonly used for the storage of cells, tissues, organs or 3D cell-based products using ultra-low temperatures, which involves the immersion in liquid nitrogen for their long-term preservation. The cryopreservation of several microencapsulated cells is usually performed by the slow freezing with the dimethyl sulfoxide (DMSO) as ...
    Evaluation of Anticancer activity of Silver Nanoparticles on the A549 Human Lung Carcinoma Cell Lines through Alamar Blue Assay
    通过阿尔玛蓝显色法评估A549人肺癌细胞系上的银纳米粒子
    作者:Nikita Sharma, Geeta Arya, R. Mankamna Kumari, Nidhi Gupta and Surendra Nimesh日期:01/05/2019,浏览量:9362,Q&A: 0
    [Abstract] Silver nanoparticles have been widely studied to possess antimicrobial as well as anticancer activity, and have found its applications in various fields including pharmaceutical industry, diagnostics, drug delivery, food industry, and others. For this purpose, several cell proliferation assays are widely used for the evaluation of anticancer ...
    Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid
    在人卵巢腹水癌细胞中监测自然杀手细胞功能
    作者:Vikash Kumar and Asok Mukhopadhyay日期:12/20/2018,浏览量:3845,Q&A: 0
    [Abstract] Natural killer (NK) cells are the major effectors of the innate immune system when activated resulting in modulation of immune response of the host defense through target cell lysis and secretion of cytokines. Precise functions of NK cells are essential for the treatment outcome of different virus infections and malignant diseases. NK cells impart ...
    Detection of Cell Death in Planarians
    真涡虫细胞死亡的检测
    作者:Nídia de Sousa and Teresa Adell日期:10/05/2018,浏览量:4508,Q&A: 0
    [Abstract] Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the ...
    An Image-based Assay for High-throughput Analysis of Cell Proliferation and Cell Death of Adherent Cells
    采用基于成像的分析法进行粘附细胞的细胞增殖和细胞死亡高通量分析
    作者:Paula Szalai and Nikolai Engedal日期:05/05/2018,浏览量:7299,Q&A: 0
    [Abstract] In this protocol, we describe a method to monitor cell proliferation and death by live-cell imaging of propidium iodide (PI)-stained adherent mammalian cells. PI is widely used to assess cell death. However, it is usually used in end-point assays. Recently, we implemented the use of PI for real-time cell death assessment by automated imaging. ...