免疫学

分类

    现刊
    Isolation of Nasal Brush Cells for Single-cell Preparations
    用于单细胞制备的鼻刷细胞的分离
    作者:Saltanat Ualiyeva, Amelia A. Boyd, Nora A. Barrett and Lora G. Bankova日期:09/20/2021,浏览量:183,Q&A: 0
    [Abstract]

    Solitary chemosensory epithelial cells are scattered in most mucosal surfaces. They are referred to as tuft cells in the intestinal mucosa, brush cells in the trachea, and solitary chemosensory and microvillous cells in the nasal mucosa. They are the primary source of IL-25 in the epithelium and are also engaged in acetylcholine generation. We

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    Parasitemia Evaluation in Mice Infected with Schistosoma mansoni
    曼氏血吸虫感染小鼠的寄生虫病评估
    作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:2154,Q&A: 0
    [Abstract]

    Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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    In vivo CD40 Silencing by siRNA Infusion in Rodents and Evaluation by Kidney Immunostaining
    小鼠体内siRNA转染CD40沉默和肾免疫染色评价
    [Abstract]

    The co-stimulatory molecule CD40 and its ligand CD40L play a key role in the regulation of immunological processes and are involved in the pathophysiology of autoimmune and inflammatory diseases. Inhibition of the CD40-CD40L axis is a promising therapy, and a number of strategies and techniques have been designed to hinder its functionality. Our

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    Analysis of Monocyte Cell Fate by Adoptive Transfer in a Murine Model of TLR7-induced Systemic Inflammation
    TLR7诱导的系统性炎症小鼠模型中通过过继转移单核细胞命运分析
    作者:Jaba Gamrekelashvili, Hermann Haller and Florian P. Limbourg日期:05/05/2021,浏览量:2687,Q&A: 0
    [Abstract]

    Myeloid plasticity is a hallmark of the innate immune response to Toll-like receptor (TLR) activation. Here, we provide a protocol for monocyte cell fate tracking by adoptive transfer in the context of systemic inflammation induced by TLR7 activation, the principal innate immune receptor sensing viral RNA in mice. Defined monocyte subsets are

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    Preparation of Nippostrongylus brasiliensis Larvae for the Study of Host Skin Response
    用于研究寄主皮肤反应的巴西日圆线虫幼虫的制备
    作者:Tiffany Bouchery, Gillian Coakley and Nicola L. Harris日期:12/20/2020,浏览量:924,Q&A: 0
    [Abstract]

    Hookworms are skin penetrating parasites, however in the laboratory the hookworm model Nippostrongylus brasiliensis, the parasite is traditionally administered subcutaneously bypassing the skin (epidermis and dermis). Here, we describe two complementary approaches for infecting mice with N. brasiliensis in order to study the skin immune responses.

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    Surgical Induction of Endometriosis in Female Mice
    雌性小鼠子宫内膜异位症的手术诱导
    作者:Alejandra Escudero-Lara, David Cabañero and Rafael Maldonado日期:09/20/2020,浏览量:1637,Q&A: 0
    [Abstract] Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous ...
    Immunohistochemistry of Kidney a-SMA, Collagen 1, and Collagen 3, in A Novel Mouse Model of Reno-cardiac Syndrome
    心肾综合征新型小鼠模型中肾脏a-SMA,胶原1和胶原3的免疫组化研究
    [Abstract] Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease (ESRD) population compared to the population as a whole thus necessitating the need for improved ...
    Real-time in vivo Imaging of LPS-induced Local Inflammation and Drug Deposition in NF-κB Reporter Mice
    LPS诱导NF-κB报告小鼠局部炎症及药物沉积的实时体内显像研究
    作者:Artur Schmidtchen and Manoj Puthia日期:08/20/2020,浏览量:2493,Q&A: 0
    [Abstract] Wound, biomaterial, and surgical infections are all characterized by a localized and excessive inflammation, motivating the development of in vivo methods focused on the analysis of local immune events. However, current inflammation models, such as the commonly used in vivo models of endotoxin-induced inflammation are based on ...
    Isolation and Quantification of Extracellular DNA from Biofluids
    生物流体中细胞外DNA的分离和定量
    作者:Ľubica Janovičová, Barbora Konečná, Barbora Vlková and Peter Celec日期:08/20/2020,浏览量:1978,Q&A: 0
    [Abstract] Extracellular DNA is studied as a diagnostic biomarker, but also as a factor involved in the pathophysiology of several diseases due to its pro-inflammatory properties. Extracellular DNA can be extracted from plasma, urine, saliva or other biofluids using standard DNA isolation procedures and specialized commercial kits. Sample preparation for ...
    RNA Extraction from Ears and Draining Lymph Nodes of Mice Infected with Leishmania amazonensis
    亚马逊利什曼原虫感染小鼠耳及引流淋巴结RNA的提取
    作者:Emilie Giraud and Evie Melanitou日期:06/05/2020,浏览量:2456,Q&A: 0
    [Abstract] Parasites of the genus Leishmania infect the mammalian hosts, including mice and humans and cause cutaneous or visceral leishmaniasis depending upon the parasite species transmitted by the vector sandfly. Leishmania amazonensis is one of the Leishmania species responsible for the cutaneous form of the disease. We have ...