微生物学

分类

    现刊
    Differential Fractionation of Erythrocytes Infected by Plasmodium berghei
    感染伯氏疟原虫红细胞的差异分离
    作者:Bénédicte Gnangnon, Véronique Peucelle and Christine Pierrot日期:06/05/2020,浏览量:1782,Q&A: 0
    [Abstract] The study of host/pathogen interactions at the cellular level during Plasmodium intra-erythrocytic cycle requires differential extraction techniques aiming to analyze the different compartments of the infected cell. Various protocols have been proposed in the literature to study specific compartments and/or membranes in the infected ...
    Model of Chemotherapy-associated Mucositis and Oral Opportunistic Infections
    化疗相关性粘膜炎与口腔机会性感染模型的建立
    作者:Takanori Sobue, Martinna Bertolini, Angela Thompson and Anna Dongari-Bagtzoglou日期:11/05/2019,浏览量:2551,Q&A: 1
    [Abstract] Oral mucositis is a common complication of cancer chemotherapy treatment. Because of the lack of relevant oral mucositis experimental models, it is not clear how chemotherapeutic agents injure the oral mucosa and if commensal microorganisms accelerate tissue damage. We developed an organotypic oral mucosa model that mimics cellular responses ...
    Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages
    研究酗酒酒精毒性对小鼠肺泡巨噬细胞影响的细胞内侵袭和杀伤试验
    作者:Victor Jimenez Jr and Fernando P Monroy日期:01/20/2019,浏览量:4115,Q&A: 0
    [Abstract] Alcohol consumption has diverse and well-documented effects on the human immune system and its ability to defend against infective agents. While pulmonary related infections can occur in healthy humans, binge alcohol use is recognized as a major health risk factor (Nelson et al., 1991). Although binge alcohol consumption has been ...
    Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines
    使用基于睡美人转座子的系统快速生成HBV复制稳定细胞系
    作者:Jin-Wei Zheng, Jia-Li Cao and Quan Yuan 日期:07/05/2018,浏览量:6237,Q&A: 0
    [Abstract] The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV. However, previous reported strategies to generate HBV-replicating cell lines, which primarily rely on random integration of exogenous DNA by ...
    Adhesion of Enteroaggregative E. coli Strains to HEK293 Cells
    肠凝聚型大肠埃希杆菌对HEK293细胞的粘附测定
    作者:Jorge Luis Ayala-Lujan and Fernando Ruiz-Perez日期:04/20/2018,浏览量:4747,Q&A: 0
    [Abstract] Enteroaggregative Escherichia coli (EAEC) is a recognized cause of acute diarrhea among both children and adults worldwide. EAEC strains are characterized by the presence of aggregative adherence fimbriae (AAF), which play a key role in pathogenesis by mediating attachment to the intestinal mucosa and by triggering host inflammatory ...
    Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ-H2AX as a Marker
    γ-H2AX作为标志物采用细胞内蛋白质印迹法(ICW)定量HeLa细胞中Colibactin相关基因毒性
    作者:Sophie Tronnet and Eric Oswald日期:03/20/2018,浏览量:5333,Q&A: 0
    [Abstract] The genotoxin colibactin is produced by several species of Enterobacteriaceae. This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells (Nougayrède et al., 2006; Taieb et al., 2016). Here we describe a method to quantify the genotoxicity of bacteria producing colibactin following a ...
    Adhesion and Invasion Assay Procedure Using Caco-2 Cells for Listeria monocytogenes
    单核细胞增生李斯特菌对Caco-2细胞的粘附和侵袭试验程序
    作者:Swetha Reddy and Frank Austin日期:05/05/2017,浏览量:12220,Q&A: 0
    [Abstract] Listeria monocytogenes is an important Gram-positive foodborne pathogen that is a particular problem in ready-to-eat food. It has an ability to survive in harsh conditions like refrigeration temperatures and high salt concentrations and is known to cross intestinal, placental and blood-brain barriers. Several cancerous cell lines like ...
    Isolation of Exosomes from Semen for in vitro Uptake and HIV-1 Infection Assays
    从精液中分离外来体用于体外摄取和HIV-1感染测定
    作者:Marisa N. Madison, Jennifer L. Welch and Chioma M. Okeoma日期:04/05/2017,浏览量:9283,Q&A: 0
    [Abstract] Exosomes are membranous extracellular nanovesicles of endocytic origin. Exosomes are known to carry host and pathogen-derived genomic, proteomic, lipidomic cargos and other extraneous molecules. Exosomes are secreted by diverse cell types into the extracellular milieu and are subsequently internalized by recipient neighboring or distal cells. Upon ...
    Transient Transfection-based Fusion Assay for Viral Proteins
    基于瞬时转染的病毒蛋白融合分析法
    作者:Melina Vallbracht, Christina Schröter, Barbara G. Klupp and Thomas C. Mettenleiter日期:03/05/2017,浏览量:9266,Q&A: 1
    [Abstract] Membrane fusion is vital for entry of enveloped viruses into host cells as well as for direct viral cell-to-cell spread. To understand the fusion mechanism in more detail, we use an infection free system whereby fusion can be induced by a minimal set of the alphaherpesvirus pseudorabies virus (PrV) glycoproteins gB, gH and gL. Here, we describe an ...
    Murine Leukemia Virus (MLV)-based Coronavirus Spike-pseudotyped Particle Production and Infection
    基于鼠白血病病毒(MLV)的冠状病毒刺突假型颗粒的生产和感染
    作者:Jean Kaoru Millet and Gary R. Whittaker日期:12/05/2016,浏览量:10954,Q&A: 0
    [Abstract] Viral pseudotyped particles (pp) are enveloped virus particles, typically derived from retroviruses or rhabdoviruses, that harbor heterologous envelope glycoproteins on their surface and a genome lacking essential genes. These synthetic viral particles are safer surrogates of native viruses and acquire the tropism and host entry pathway ...