联合作者(
Francesca Cantini 1 protocol

Alexandre M. J. J. Bonvin Computational Structural Biology group, Bijvoet Center for Biomolecular Research, Faculty of Science Chemistry, Utrecht University, the Netherlands
2 protocols

Vincenzo Maione 1 protocol
联合评审(
Khyati Hitesh Shah Sutro BioPharma
21 protocols

评审
Andrea Saponaro
  • Post-Doc, University of Milan
研究方向
  • 生物物理学
  • 已发表 protocol 2

教育背景

University of Milan

发表论文

• Santoro B., Hu L., Liu H., Saponaro A., Pian P., Piskorowski R. A., Moroni A., Siegelbaum S. A., “Tetratricopeptide Repeat-Containing Rab8b Interacting Protein (TRIP8b) regulates hyperpolarization-activated cyclic nucleotide-regulated 1 (HCN1) channel trafficking and gating through two distinct C-terminal interaction sites”, The Journal of Neuroscience, 2011, 31(11): 4074-4086 [doi:10.1523/JNEUROSCI.5707-10.2011].
• Smeazzetto S., Saponaro A., Young H.S., Moncelli M.R., Thiel G., “Structure-Function Relation of Phospholamban: Modulation of Channel Activity as a Potential Regulator of SERCA Activity”, PLoS One., 2013, 8(1):e52744 [doi: 10.1371/journal.pone.0052744].
• Hu L., Santoro B., Saponaro A., Liu H., Moroni A., Siegelbaum S. A., “Binding of the auxiliary subunit TRIP8b to HCN channels shift the mode of action of cAMP”, The Journal of General Physiology, 2013, 142(6): 599-612 [doi: 10.1085/jgp.201311013].
• Saponaro A., Pauleta S.R., Cantini F., Hu L., Matzapetakis M., Hammann C., Donadoni C., Thiel G., Banci L., Santoro B., Moroni A. “Structural basis for the mutual antagonism of cAMP and TRIP8b in regulating HCN channel function”, Proc Natl Acad Sci U S A., 2014, 111(40) :14577-82 [doi: 10.1073/pnas.1410389111].
• Weißgraeber S, Saponaro A., Thiel G., Hamacher K. “A reduced mechanical model for cAMP-modulated gating in HCN channels”. Sci Rep., 2017, 7:40168 [doi: 10.1038/srep40168].
• Saponaro A., Porro A., Chaves-Sanjuan A., Nardini M., Rauh O., Thiel G., Moroni A. “Fusicoccin Activates KAT1 Channels by Stabilizing Their Interaction with 14-3-3 Proteins”. Plant Cell. 2017, 29(10):2570-2580. [doi: 10.1105/tpc.17.00375].
• Saponaro A., Cantini F., Porro A., Bucchi A., DiFrancesco D., Maione V., Donadoni C., Introini B., Mesirca P., Mangoni M., Thiel G., Banci L., Santoro B., Moroni A. “A synthetic peptide that prevents cAMP regulation in mammalian Hyperpolarization-activated Cyclic Nucleotide-regulated (HCN) channels”. Elife. 2018, pii: e35753. doi: 10.7554/eLife.35753.
• Groß C., Saponaro A., Santoro B., Moroni A., Thiel G., Hamacher K. "Mechanical transduction of cytoplasmic-to-transmembrane-domain movements in a hyperpolarization-activated cyclic nucleotide–gated cation channel" J Biol Chem. 2018, pii: jbc.RA118.002139. doi: 10.1074/jbc.RA118.002139.
• Saponaro A. "Isothermal Titration Calorimetry: a Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution". 2018, Bio-protocol, Vol 8, Iss 15. doi: 10.21769/BioProtoc.2957.
• Alberio L., Locarno A., Saponaro A., Romano E., Bercier V., Albadri S., Del Bene F., Simeaoni F., Moleri S., Beltrame M., Pelucchi S., MarcellO E., Di Luca M., Kukovetz K., Boender AJ.,Luo S., Moutal A., Ji Y., Khanna R., Colecraft H.M., Thiel G., Tonini R., Moroni A. "A light-gated K+ channel for sustained neuronal inhibition in freely moving animals". Nat Methods. 2018 Nov;15(11):969-976. doi: 10.1038/s41592-018-0186-9.
• Porro A, Saponaro A, Gasparri F, Bauer D, Gross C, Pisoni M, Abbandonato G, Hamacher K, Santoro B, Thiel G, Moroni A. “The HCN domain couples voltage gating and cAMP response in hyperpolarization-activated cyclic nucleotide-gated channels”. Elife. 2019 Nov 26;8. pii: e49672. doi: 10.7554/eLife.49672.
• Brocca P, Saponaro A, Introini B, Rondelli V, Pannuzzo M, Raciti D, Corti M, Raudino A. “Protein Adsorption at the Air-Water Interface by a Charge Sensing Interferometric Technique”. Langmuir. 2019 Dec 10;35(49):16087-16100. doi: 10.1021/acs.langmuir.9b02201. Epub 2019 Nov 19.
• Kukovetz K, Hertel B, Schvarcz CR, Saponaro A, Manthey M, Burk U, Greiner T, Steward GF, Van Etten JL,Moroni A, Thiel G, Rauh O. “A Functional K + Channel from Tetraselmis Virus 1, a Member of the Mimiviridae”. Viruses, 2020 12(10):1107. doi: 10.3390/v12101107.
• Nardone V, Chaves-Sanjuan A, Lapi M, Airoldi C, Saponaro A, Pasqualato S, Dolfini D, Camilloni C, Bernardini A, Gnesutta N, Mantovani R, Nardini M. “Structural Basis of Inhibition of the Pioneer Transcription Factor NF-Y by Suramin”. Cells. 2020 9(11):2370. doi: 10.3390/cells9112370.
• Porro A, Binda A, Pisoni M, Donadoni C, Rivolta I, Saponaro A. “Rational design of a mutation to investigate the role of the brain protein TRIP8b in limiting the cAMP response of HCN channels in neurons”. J General Physiol. 2020, doi: 10.1085/jgp.202012596.
• Saponaro A, Maione V, Bonvin A. M. J. J., Cantini F. “Understanding Docking Complexes of Macromolecules Using HADDOCK: The Synergy between Experimental Data and Computations”. Bio-protocol, 2020, Vol 10, Iss 20. doi: 10.21769/BioProtoc.3793.
• Porro A, Thiel G, Moroni A, Saponaro A. “cyclic AMP Regulation and Its Command in the Pacemaker Channel HCN4”. Front. Physiol. 2020, doi: 10.3389/fphys.2020.00771.
已发表 protocol 2篇
Understanding Docking Complexes of Macromolecules Using HADDOCK: The Synergy between Experimental Data and Computations
使用HADDOCK了解高分子对接复合物:实验数据与计算之间的协同作用

作者:Andrea Saponaro, Vincenzo Maione, Alexandre M. J. J. Bonvin and Francesca Cantini日期:10/20/2020,浏览量:1565,Q&A: 0
This protocol illustrates the modelling of a protein-peptide complex using the synergic combination of in silico analysis and experimental results. To this end, we use the integrative modelling software HADDOCK, which possesses the powerful ...
Isothermal Titration Calorimetry: A Biophysical Method to Characterize the Interaction between Label-free Biomolecules in Solution
等温滴定量热法:一种检测溶液中非标记生物分子相互作用的生物物理方法

作者:Andrea Saponaro日期:08/05/2018,浏览量:17982,Q&A: 0
This protocol can be applied to analyze the direct interaction between a soluble protein and a target ligand molecule using Isothermal Titration Calorimetry (ITC, Malvern). ITC allows the biophysical characterization of binding between label-free, ...
参审 protocol 1篇
Purification of Mitochondrial Ribosomes with the Translocase Oxa1L from HEK Cells
用转位酶Oxa1L纯化HEK细胞线粒体核糖体
作者:Hanting Yang and Nirupa Desai日期:08/05/2021,浏览量:985,Q&A: 0

Mitochondrial ribosomes (mitoribosomes) perform protein synthesis inside mitochondria, the organelles responsible for energy conversion and adenosine triphosphate (ATP) production in eukaryotic cells. To investigate their functions and structures,

...
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