• 卷册 12, 2022
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Image Credit: BioProtoc.4459

现期刊物(2022)

卷册: 12, 期号: 13

生物化学

Whole-mount Senescence-Associated Beta-Galactosidase (SA-β-GAL) Activity Detection Protocol for Adult Zebrafish 成年斑马鱼的整体衰老相关 β-半乳糖苷酶 (SA-β-GAL) 活性检测方案 作者:Marta Marzullo, Mounir El Maï and Miguel Godinho Ferreira日期:07/05/2022,浏览量:80,Q&A: 0

Senescence-associated beta-galactosidase (SA-β-GAL) is an enzyme that accumulates in the lysosomes of senescent cells, where it hydrolyses β-galactosides. With p16, it represents a well-recognized biomarker used to assess senescence both in vivo and in cell culture. The use of a chromogenic substrate, such as

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生物工程

A Robust Nanoparticle-based Magnetic Separation Method for Intact Lysosomes 一种用于分离完整溶酶体的基于纳米颗粒的磁分离方法 作者:The Son Le, Mari Takahashi and Shinya Maenosono日期:07/05/2022,浏览量:112,Q&A: 0

Lysosome isolation is a preresiquite for identifying lysosomal protein composition by mass spectroscopic analysis, to reveal lysosome functions, and their involvement in some diseases. Magnetic nanoparticle-based fractionation has received great attention for lysosome isolation, owing to its high efficiency, purity, and preservation of lysosomal

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VirScan: High-throughput Profiling of Antiviral Antibody Epitopes Vir Scan: 抗病毒抗体表位的高通量分析 作者:Ellen L. Shrock, Christine L. Shrock and Stephen J. Elledge日期:07/05/2022,浏览量:96,Q&A: 0

Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq) method for profiling the specificities of human antiviral antibodies. Accompanying this protocol is

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细胞生物学

Experimental Models for Cold Exposure of Muscle in vitro and in vivo 体外和体内肌肉低温暴露实验模型 作者:Tiril Schjølberg, Lucia Asoawe, Solveig Krapf, Arild C. Rustan, G. Hege Thoresen and Fred Haugen日期:07/05/2022,浏览量:52,Q&A: 0

Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of cold exposure in muscle, and since adequate methods were missing, we established two experimental cold

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发育生物学

In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labeling 利用CRISPR/Cas9 策略和基于BioID的邻近标记研究果蝇幼虫大脑内源性蛋白相互作用体的体内特性 作者:Ezgi Uçkun, Georg Wolfstetter, Johannes Fuchs and Ruth H. Palmer日期:07/05/2022,浏览量:65,Q&A: 0

Understanding protein-protein interactions (PPIs) and interactome networks is essential to reveal molecular mechanisms mediating various cellular processes. The most common method to study PPIs in vivo is affinity purification combined with mass spectrometry (AP–MS). Although AP–MS is a powerful method, loss of weak and transient

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免疫学

Serological Measurement of Poly-IgA Immune Complex Levels in IgA Nephropathy and IgA Vasculitis IgA 肾病和 IgA 血管炎中多聚 IgA 免疫复合物水平的血清学测定 作者:Xue Zhang, Jicheng Lv, Pan Liu, Xinfang Xie, Xinyan Li, Hong Zhang and Jing Jin日期:07/05/2022,浏览量:92,Q&A: 0

Both IgA nephropathy and IgA vasculitis, formerly known as Henoch-Schӧnlein purpura, are immune deposition diseases. IgA nephropathy is caused by the deposition of aberrantly formed poly-IgA complexes from blood circulation to the kidney glomerulus; IgA vasculitis is characterized by IgA-dominant immune deposits to small vessels of the skin

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微生物学

A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differences in Fitness during Plant Infection 基于β-葡萄糖醛酸酶 (GUS) 的细菌竞争测定以评估植物感染期间适应性的细微差异 作者:Julien S. Luneau, Laurent D. Noël, Emmanuelle Lauber and Alice Boulanger日期:07/05/2022,浏览量:47,Q&A: 0

Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences that would not otherwise be detectable. In the frame of host-pathogens interactions, they can be

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分子生物学

HaloChIP-seq for Antibody-Independent Mapping of Mouse Transcription Factor Cistromes in vivo HaloChIP-seq用于体内小鼠转录因子 Cistromes 的抗体独立映射 作者:Ann Louise Hunter, Antony D. Adamson, Toryn M. Poolman, Magdalena Grudzien, Andrew S. I. Loudon, David W. Ray and David A. Bechtold日期:07/05/2022,浏览量:67,Q&A: 0

Chromatin immunoprecipitation (ChIP) maps, on a genome-wide scale, transcription factor binding sites, and the distribution of other chromatin-associated proteins and their modifications. As such, it provides valuable insights into mechanisms of gene regulation. However, successful ChIP experiments are dependent on the availability of a

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神经科学

Automated Quantification of Multiple Cell Types in Fluorescently Labeled Whole Mouse Brain Sections Using QuPath QuPath对荧光标记的整个小鼠大脑切片中的多种细胞类型进行自动定量 作者:Jo-Maree Courtney, Gary P. Morris, Elise M. Cleary, David W. Howells and Brad A. Sutherland日期:07/05/2022,浏览量:154,Q&A: 0

The quantification of labeled cells in tissue sections is crucial to the advancement of biological knowledge. Traditionally, this was a tedious process, requiring hours of careful manual counting in small portions of a larger tissue section. To overcome this, many automated methods for cell analysis have been developed. Recent advances in whole

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