• 卷册 12, 2022
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  • 卷册 9, 2019
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  • 卷册 6, 2016
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  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.4422

现期刊物(2022)

卷册: 12, 期号: 10

生物工程

Preparation of Cas9 Ribonucleoproteins for Genome Editing 用于基因组编辑的 Cas9 核糖核蛋白的制备 作者:Sheng-Wei Lin, Viet Quoc Nguyen and Steven Lin日期:05/20/2022,浏览量:31,Q&A: 0

Genome editing by the delivery of pre-assembled Cas9 ribonucleoproteins (Cas9 RNP) is an increasingly popular approach for cell types that are difficult to manipulate genetically by the conventional plasmid and viral methods. Cas9 RNP editing is robust, precise, capable of multiplexing, and free of genetic materials. Its transient presence in

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Immunomagnetic Isolation and Enrichment of Microvascular Endothelial Cells from Human Adipose Tissue 人体脂肪组织微血管内皮细胞的免疫磁分离和富集 作者:Jeremy A. Antonyshyn, Vienna Mazzoli, Meghan J. McFadden, Anthony O. Gramolini, Stefan O. P. Hofer, Craig A. Simmons and J. Paul Santerre日期:05/20/2022,浏览量:52,Q&A: 0

Human adipose tissue-resident microvascular endothelial cells are not only garnering attention for their emergent role in the pathogenesis of obesity-related metabolic disorders, but are also of considerable interest for vascular tissue engineering due, in part, to the abundant, accessible, and uniquely dispensable nature of the tissue. Here, we

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生物物理学

Purification of Mitochondrial Ribosomal Complexes from Trypanosoma cruzi and Leishmania tarentolae for Cryo-EM Analysis 克氏锥虫和塔氏利什曼原虫线粒体核糖体复合物的纯化及低温电子显微镜分析 作者:Stéphanie Durrieu-Gaillard, Marie Sissler and Yaser Hashem日期:05/20/2022,浏览量:25,Q&A: 0

Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis, caused by Trypanosoma cruzi, Trypanosoma brucei, and various Leishmania spp., respectively. They harbor a single large mitochondrion that is essential for the survival of the parasite. Interestingly,

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癌症生物学

A Flow Cytometry-Based Method for Analyzing DNA End Resection in G0- and G1-Phase Mammalian Cells 一种基于流式细胞术的分析 G0和G1期哺乳动物细胞中 DNA 末端切除的方法 作者:Bo-Ruei Chen, Jessica K. Tyler and Barry P. Sleckman日期:05/20/2022,浏览量:29,Q&A: 0

DNA double strand breaks (DSBs) constantly arise in cells during normal cellular processes or upon exposure to genotoxic agents, and are repaired mostly by homologous recombination (HR) and non-homologous end joining (NHEJ). One key determinant of DNA DSB repair pathway choice is the processing of broken DNA ends to generate single strand DNA

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A Highly Sensitive Method to Efficiently Profile the Histone Modifications of FFPE Samples 一种高效分析 FFPE 样品组蛋白修饰的高灵敏度方法 作者:Linxuan Zhao, Vamsi Krishna Polavarapu, Ram Prakash Yadav, Pengwei Xing and Xingqi Chen日期:05/20/2022,浏览量:33,Q&A: 0

The majority of biopsies in both basic research and translational cancer studies are preserved in the format of archived formalin-fixed paraffin-embedded (FFPE) samples. Profiling histone modifications in archived FFPE tissues is critically important to understand gene regulation in human disease. The required input for current genome-wide

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细胞生物学

Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat 分离成年小鼠和大鼠的心肌细胞 作者:Huiliang Zhang and Peter S. Rabinovitch日期:05/20/2022,浏览量:36,Q&A: 0

The isolation of intact single adult cardiomyocytes from model animals, mouse and rat, is an essential tool for cardiac molecular and cellular research. While several methods are reported for adult mouse cardiomyocyte isolation, the viability and yield of the isolated cells have been variable. Here, we describe step-by-step protocols for high

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CRISPR/Cas9 Gene Editing of HeLa Cells to Tag Proteins with mNeonGreen mNeonGreen对 HeLa 细胞进行 CRISPR/Cas9 基因编辑以标记蛋白质 作者:Sachin Surve and Alexander Sorkin日期:05/20/2022,浏览量:81,Q&A: 0

Subcellular localization dynamics of proteins involved in signal transduction processes is crucial in determining the signaling outcome. However, there is very limited information about the localization of endogenous signaling proteins in living cells. For example, biochemical mechanisms underlying the signaling pathway from epidermal growth

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发育生物学

Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle 从小鼠虹膜角膜角制备单细胞悬液 作者:Benjamin R. Thomson and Susan E. Quaggin日期:05/20/2022,浏览量:23,Q&A: 0

Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified novel cell types within the retina, anterior chamber, and iridocorneal angle and explored

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免疫学

Protocol to Induce Follicular T Helper Cells, Germinal Centers, and Skin Lesions in Mouse Models for Skin Blistering Diseases 在皮肤起泡疾病小鼠模型中诱导滤泡 T 辅助细胞、生发中心和皮肤损伤的方法 作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Katja Bieber, Juergen Westermann and Kathrin Kalies日期:05/20/2022,浏览量:79,Q&A: 0

Autoreactive T cells in autoantibody-mediated autoimmune diseases can be divided into two major subsets: (i) follicular T helper cells (Tfh) that provide T cell help in germinal centers (GC) and (ii) effector T (Teff) cells that immigrate into peripheral tissue sites such as the skin and mediate local inflammation. To study the sequence of events

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微生物学

Flow Cytometry-based Measurement of Reactive Oxygen Species in Cyanobacteria 基于流式细胞仪的蓝藻活性氧测量 作者:Soumila Mondal and Shailendra P. Singh日期:05/20/2022,浏览量:36,Q&A: 0

Cyanobacteria are Gram-negative oxygen-producing photosynthetic bacteria that are useful in the pharmaceutical and biofuel industries. Monitoring of oxidative stress under fluctuating environmental conditions is important for determining the fitness, survival, and growth of cyanobacteria in the laboratory as well as in large scale cultivation

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Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues 从沙门氏菌鼠伤寒血清中纯化细菌淀粉样蛋白“Curli”并检测受感染宿主组织中的 Curli 作者:Murugesan Sivaranjani, Elizabeth G Hansen, Sumudu R. Perera, Pamela A. Flores, Çagla Tükel and Aaron P. White日期:05/20/2022,浏览量:30,Q&A: 0

Microbiologists are learning to appreciate the importance of “functional amyloids” that are produced by numerous bacterial species and have impacts beyond the microbial world. These structures are used by bacteria to link together, presumably to increase survival, protect against harsh conditions, and perhaps to influence cell-cell

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神经科学

Vestibular Organ Dissection and Whole-Mount Immunolabeling in Mouse 前庭器官解剖和整体免疫标记 在鼠标中 作者:Timothy S. Balmer and Laurence O. Trussell日期:05/20/2022,浏览量:32,Q&A: 0

The vestibular sensory apparatus contained in the inner ear is a marvelous evolutionary adaptation for sensing movement in 3 dimensions and is essential for an animal’s sense of orientation in space, head movement, and balance. Damage to these systems through injury or disease can lead to vertigo, Meniere’s disease, and other disorders

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Identification and Monitoring of Nucleotide Repeat Expansions Using Southern Blotting in Drosophila Models of C9orf72 Motor Neuron Disease and Frontotemporal Dementia 应用Southern Blotting鉴定和监测C9orf72运动神经元疾病和额颞叶痴呆模型的核苷酸重复扩增 作者:Joanne L. Sharpe, Nikki S. Harper and Ryan J. H. West日期:05/20/2022,浏览量:20,Q&A: 0

Repeat expansion diseases, including fragile X syndrome, Huntington’s disease, and C9orf72-related motor neuron disease and frontotemporal dementia, are a group of disorders associated with polymorphic expansions of tandem repeat nucleotide sequences. These expansions are highly repetitive and often hundreds to thousands of repeats in

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植物科学

In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions 评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定 作者:Hendry Susila, Snježana Jurić, Katarzyna Gawarecka, Kyung Sook Chung, Suhyun Jin, Soo-Jin Kim, Zeeshan Nasim, Geummin Youn and Ji Hoon Ahn日期:05/20/2022,浏览量:35,Q&A: 0

Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids participate in these interactions remains challenging. In vitro assays to assess the physical

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干细胞

Optimized CRISPR-Cas9-based Strategy for Complex Gene Targeting in Murine Embryonic Stem Cells for Germline Transmission 用于生殖系传递的基于 CRISPR-Cas9 的小鼠胚胎干细胞中优化的复杂基因靶向策略 作者:Thomas J. O'Neill, Daniel Krappmann and Andreas Gewies日期:05/20/2022,浏览量:72,Q&A: 0

Although CRISPR-Cas9 genome editing can be performed directly in single-cell mouse zygotes, the targeting efficiency for more complex modifications such as the insertion of two loxP sites, multiple mutations in cis, or the precise insertion or deletion of longer DNA sequences often remains low (Cohen, 2016). Thus, targeting and validation of

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