• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.3987

往期刊物(2021)

卷册: 11 期号: 8

生物化学

Quantitative Characterization of the Amount and Length of (1,3)-β-D-glucan for Functional and Mechanistic Analysis of Fungal (1,3)-β-D-glucan Synthase 通过(1,3)-β-d-葡聚糖量和长度的定量表征对真菌(1,3)-β-d-葡聚糖合酶进行功能和机理分析 作者:Abhishek Chhetri, Anna Loksztejn and Kenichi Yokoyama日期:04/20/2021,浏览量:801,Q&A: 0

(1,3)-β-d-Glucan synthase (GS) is an essential enzyme for fungal cell wall biosynthesis that catalyzes the synthesis of (1,3)-β-d-glucan, a major and vital component of the cell wall. GS is a proven target of antifungal antibiotics including FDA-approved echinocandin derivatives; however, the function and mechanism of GS remain largely

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Optimized Recombinant Production of Secreted Proteins Using Human Embryonic Kidney (HEK293) Cells Grown in Suspension 利用悬浮培养的人胚胎肾(HEK293)细胞优化分泌蛋白的重组生产 作者:Silvia Faravelli, Matteo Campioni, Martina Palamini, Anselmo Canciani, Antonella Chiapparino and Federico Forneris日期:04/20/2021,浏览量:1007,Q&A: 0

Recombinant proteins are an essential milestone for a plethora of different applications ranging from pharmaceutical to clinical, and mammalian cell lines are among the currently preferred systems to obtain large amounts of proteins of interest due to their high level of post-translational modification and manageable large-scale production. In

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癌症生物学

A Robust Mammary Organoid System to Model Lactation and Involution-like Processes 一个健康的乳腺有机样系统来模拟哺乳和退化样过程 作者:Elsa Charifou, Jakub Sumbal, Zuzana Koledova, Han Li and Aurélie Chiche日期:04/20/2021,浏览量:800,Q&A: 0

The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding

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细胞生物学

AIMTOR, a BRET Biosensor for Live Recording of mTOR Activity in Cell Populations and Single Cells 一种用于实时记录细胞群和单个细胞中mTOR活性的BRET生物传感器AIMTOR 作者:Nathalie Bouquier, Enora Moutin, Julie Perroy and Vincent Ollendorff日期:04/20/2021,浏览量:727,Q&A: 0

Mammalian target of rapamycin (mTOR) controls many crucial cellular functions, including protein synthesis, cell size, energy metabolism, lysosome and mitochondria biogenesis, and autophagy. Consequently, deregulation of mTOR signaling plays a role in numerous pathological conditions such as cancer, metabolic disorders and neurological diseases.

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发育生物学

Production of Phenotypically Uniform Human Cerebral Organoids from Pluripotent Stem Cells 从多能干细胞生产表型一致的人类大脑类器官 作者:Adam Sivitilli, Parisa Ghiasi and Liliana Attisano日期:04/20/2021,浏览量:658,Q&A: 0

Recent advances in stem cell technology have allowed researchers to generate 3D cerebral organoids (COs) from human pluripotent stem cells (hPSCs). Indeed, COs have provided an unprecedented opportunity to model the developing human brain in a 3D context, and in turn, are suitable for addressing complex neurological questions by leveraging

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Development of a Chemical Reproductive Aging Model in Female Rats 雌性大鼠化学生殖衰老模型的建立 作者:Nayara Pestana-Oliveira, Ruither O. G. Carolino, Bruna Kalil-Cutti, Cristiane M. Leite, Litamara C. Dalpogeto, Bruna Balbino De Paula, John P. Collister and Janete Anselmo-Franci日期:04/20/2021,浏览量:764,Q&A: 0

Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from reproductive to non-reproductive life. It is usually characterized by neuroendocrine, metabolic and

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微生物学

A Sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA while Preserving Cellular DNA 一种在保留细胞DNA的同时定量检测乙型肝炎病毒共价闭合环状(ccc)DNA的灵敏、特异PCR方法 作者:Benno Zehnder, Stephan Urban and Thomas Tu日期:04/20/2021,浏览量:570,Q&A: 0

Hepatitis B virus (HBV) is the major cause of liver diseases and liver cancer worldwide. After infecting hepatocytes, the virus establishes a stable episome (covalently closed circular DNA, or cccDNA) that serves as the template for all viral transcripts. Specific and accurate quantification of cccDNA is difficult because infected cells contain

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Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay 用CCF4-AM FRET法检测RAW264.7巨噬细胞中海洋分枝杆菌的细胞质移位 作者:Javier Aguilera and Jianjun Sun日期:04/20/2021,浏览量:485,Q&A: 0
The CCF4-AM Förster resonance energy transfer (FRET) assay is a sensitive approach to measure bacterial cytosolic translocation in live cells. The FRET pair hydroxycoumarin (donor) and fluorescein (acceptor) are linked by a CCF4-AM β-lactam ring, the substrate of β-lactamase. The exogenously added, neutral charged-FRET reagent can diffuse across ...
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Single Cell Analysis and Sorting of Aspergillus fumigatus by Flow Cytometry 用流式细胞仪对烟曲霉进行单细胞分析和分选 作者:Gareth Howell and Robert-Jan Bleichrodt日期:04/20/2021,浏览量:605,Q&A: 0

Experimental results in fungal biology research are usually obtained as average measurements across whole populations of cells, whilst ignoring what is happening at the single cell level. Microscopy has allowed us to study single-cell behavior, but it has low throughput and cannot be used to select individual cells for downstream experiments. Here

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Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages 筛选与治疗相关的噬菌体的溶原活性 作者:Fernando L. Gordillo Altamirano and Jeremy J. Barr日期:04/20/2021,浏览量:825,Q&A: 1

Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal

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神经科学

A Method to Quantify Drosophila Behavioral Activities Induced by GABAA Agonist Muscimol GABAA激动剂毒蝇蕈醇诱导果蝇行为活动的量化方法 作者:M. Rezaul Karim日期:04/20/2021,浏览量:401,Q&A: 0

Muscimol is a psychoactive isoxazole derived from the mushroom Amanita muscaria. As a potent GABAA receptor agonist, muscimol suppresses the activity of the central nervous system, reduces anxiety and induces sleep. We investigated the effects of muscimol on Drosophila behavior. Drosophila behavioral assays are powerful tools that are used to

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Single or Repeated Ablation of Mouse Olfactory Epithelium by Methimazole 甲巯咪唑单次或多次消融小鼠嗅上皮细胞 作者:Sofia Håglin, Staffan Bohm and Anna Berghard日期:04/20/2021,浏览量:469,Q&A: 0

Odor-detecting olfactory sensory neurons residing in the nasal olfactory epithelium (OE) are the only neurons in direct contact with the external environment. As a result, these neurons are subjected to chemical, physical, and infectious insults, which may be the underlying reason why neurogenesis occurs in the OE of adult mammals. This feature

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Single-unit Recording in Awake Behaving Non-human Primates 非人类灵长类动物清醒时的单细胞记录 作者:Mengxi Yun, Masafumi Nejime and Masayuki Matsumoto日期:04/20/2021,浏览量:428,Q&A: 0

Non-human primates (NHPs) have been widely used as a species model in studies to understand higher brain functions in health and disease. These studies employ specifically designed behavioral tasks in which animal behavior is well-controlled, and record neuronal activity at high spatial and temporal resolutions while animals are performing the

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Transfection and Activation of CofActor, a Light and Stress Gated Optogenetic Tool, in Primary Hippocampal Neuron Cultures 一种在原代海马神经元培养能辅助因子的转染和激活的光和应激门控的光遗传学工具 作者:Wyatt P. Bunner, Rachel Dodson, Robert M. Hughes and Erzsebet M. Szatmari日期:04/20/2021,浏览量:477,Q&A: 0

Proteins involved in neurodegeneration can be coupled with optogenetic reagents to create rapid and sensitive reporters to provide insight into the biochemical processes that mediate the progression of neurodegenerative disorders, including Alzheimer’s Disease (AD). We have recently developed a novel optically-responsive tool (the

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系统生物学

Computational Analysis and Phylogenetic Clustering of SARS-CoV-2 Genomes SARS-CoV-2基因组计算分析及系统进化聚类分析 作者:Bani Jolly and Vinod Scaria日期:04/20/2021,浏览量:919,Q&A: 0

COVID-19, the disease caused by the novel SARS-CoV-2 coronavirus, originated as an isolated outbreak in the Hubei province of China but soon created a global pandemic and is now a major threat to healthcare systems worldwide. Following the rapid human-to-human transmission of the infection, institutes around the world have made efforts to generate

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