• 卷册 11, 2021
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  • 卷册 9, 2019
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  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
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  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.4049

往期刊物(2021)

卷册: 11 期号: 11

生物物理学

Preparation of Doublet Microtubule Fraction for Single Particle Cryo-electron Microscopy 单粒子低温电子显微镜双态微管馏分的制备 作者:Corbin Black, Daniel Chen Dai, Katya Peri, Muneyoshi Ichikawa and Khanh Huy Bui日期:06/05/2021,浏览量:1753,Q&A: 0

Over the years, studying the ultrastructure of the eukaryotic cilia/flagella using electron microscopy (EM) has contributed significantly toward our understanding of ciliary function. Major complexes in the cilia, such as inner and outer dynein arms, radial spokes, and dynein regulatory complexes, were originally discovered by EM. Classical

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细胞生物学

Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium 果蝇翅成虫上皮中Wg运输的可视化和定量研究 作者:Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross日期:06/05/2021,浏览量:1390,Q&A: 0

Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the endosomal pathway. Long-range Wg secretion and spread depend on secondary secretion from endosomal

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Isolation of First-Trimester and Full-term Human Placental Hofbauer Cells 妊娠早期和足月人胎盘霍夫鲍尔细胞的分离 作者:Anna Appios, Jake R. Thomas and Naomi McGovern日期:06/05/2021,浏览量:1716,Q&A: 0

The placenta is the crucial organ that regulates the health of both mother and fetus during pregnancy. The human placenta is composed of villous tree-like structures that embed into the maternal decidua. Within the stroma of the villi resides a population of fetally-derived macrophages, the Hofbauer cells (HBC). HBC are the only fetal immune cells

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发育生物学

Analysis of Caenorhabditis elegans Sperm Number, Size, Activation, and Mitochondrial Content 秀丽隐杆线虫精子数量、大小、活化及线粒体含量分析 作者:Amy M. Hammerquist, Chia-An Yen and Sean P. Curran日期:06/05/2021,浏览量:1386,Q&A: 0

Infertility is a widespread and often unexplained issue. Studying reproduction using C. elegans males offers insight into the influence of individual factors on male fertility in humans. We have created a collection of protocols to assess several aspects of C. elegans sperm quality, including number, size, rate of activation, and mitochondrial

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Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands 果蝇幼虫唾液腺分泌颗粒大小的定量研究 作者:Cheng-I J. Ma and Julie A. Brill日期:06/05/2021,浏览量:1322,Q&A: 0

Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in cross-sectional area after maturation. Therefore, we developed a live imaging microscopy approach

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免疫学

Multi-color Flow Cytometry for Comprehensive Analysis of the Tumor Immune Infiltrate in a Murine Model of Breast Cancer 多色流式细胞术综合分析乳腺癌小鼠模型中肿瘤免疫渗透 作者:Ana S. Almeida, Miriam R. Fein and Mikala Egeblad日期:06/05/2021,浏览量:1678,Q&A: 0

Flow cytometry is a popular laser-based technology that allows the phenotypic and functional characterization of individual cells in a high-throughput manner. Here, we describe a detailed procedure for preparing a single-cell suspension from mammary tumors of the mouse mammary tumor virus-polyoma middle T (MMTV-PyMT) and analyzing these cells by

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微生物学

Sex-specific Separation of Plasmodium falciparum Gametocyte Populations 恶性疟原虫配子细胞群体的性别特异性分离 作者:Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier日期:06/05/2021,浏览量:1257,Q&A: 0

Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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分子生物学

Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions 培养神经元细胞成细胞体和神经突部分的机械分离 作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:1682,Q&A: 0

Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the

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神经科学

In vivo Fluorescence Imaging of Extracellular ATP in the Mouse Cerebral Cortex with a Hybrid-type Optical Sensor 混合型光传感器对小鼠大脑皮层细胞外ATP的体内荧光成像 作者:Nami Kitajima, Kenji Takikawa, Hiroshi Sekiya, Daisuke Asanuma, Hirokazu Sakamoto, Shigeyuki Namiki, Masamitsu Iino and Kenzo Hirose日期:06/05/2021,浏览量:1331,Q&A: 0

Adenosine 5’-triphosphate (ATP) works as an extracellular signaling molecule for cells in the brain, such as neurons and glia. Cellular communication via release of ATP is involved in a range of processes required for normal brain functions, and aberrant communication is associated with brain disorders. To investigate the mechanisms

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Objective Quantitation of Focal Sweating Areas Using a Mouse Sweat-assay Model 小鼠汗液分析模型对局部出汗区域的客观定量 作者:Choongjin Ban and Dae-Hyuk Kwoen日期:06/05/2021,浏览量:1187,Q&A: 0

In vivo sweat quantitation assays are required for the development of drugs for the management of focal hyperhidrosis before clinical trials; however, in vivo assays, particularly mouse models, are rare. Even in sweat assays using mice, sweating is quantitated by manually counting the number of sweating spots, which can contribute to various

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植物科学

Rice Root Hair Phenotypes Imaged by Cryo-SEM 水稻根毛表型的低温扫描电镜成像 作者:Haiting Yan, Yue Wang, Jingrong Zhang, Xinru Cui, Jiasong Wu, Jie Zhou, Yuan Chen, Jia Lu, Ruiyang Guo, Maggie Ou, Hongxu Lai and Zhiming Yu日期:06/05/2021,浏览量:1665,Q&A: 0

Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content. In contrast to traditional SEM, cryo-SEM requires no sample pretreatment processes; thus, we can

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干细胞

Muscle Cryoinjury and Quantification of Regenerating Myofibers in Mice 小鼠肌肉低温损伤及再生肌纤维的定量研究 作者:Yori Endo, Mehran Karvar and Indranil Sinha日期:06/05/2021,浏览量:1388,Q&A: 0

Cryoinjury, or injury due to freezing, is a method of creating reproducible, local injuries in skeletal muscle. This method allows studying the regenerative response following muscle injuries in vivo, thus enabling the evaluation of local and systemic factors that influence the processes of myofiber regeneration. Cryoinjuries are applicable to the

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Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis 小鼠多能干细胞衍生干细胞样结构的生成:植入术后胚胎发生的体外模型 作者:Adriano Bolondi, Leah Haut, Seher Ipek Gassaloglu, Polly Burton, Helene Kretzmer, René Buschow, Alexander Meissner, Bernhard G. Herrmann and Jesse V. Veenvliet日期:06/05/2021,浏览量:1513,Q&A: 0

Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several complementary in vitro systems comprising self-organized assemblies of mouse embryonic stem cells, such

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Generation and Maintenance of Homogeneous Human Midbrain Organoids 同种人类中脑类器官的生成与维持 作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:2060,Q&A: 0

Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

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系统生物学

Detecting Differentially Methylated Promoters in Genes Related to Disease Phenotypes Using R 利用R检测与疾病表型相关基因的差异甲基化启动子 作者:Jordi Martorell Marugán and Pedro Carmona-Sáez日期:06/05/2021,浏览量:1211,Q&A: 0

DNA methylation in gene promoters plays a major role in gene expression regulation, and alterations in methylation patterns have been associated with several diseases. In this context, different software suites and statistical methods have been proposed to analyze differentially methylated positions and regions. Among them, the novel statistical

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Simplified Epigenome Profiling Using Antibody-tethered Tagmentation 基于抗体系标记的简化表观基因组图谱 作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:1635,Q&A: 0

We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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