• 卷册 11, 2021
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  • 卷册 5, 2015
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  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.4170

生物化学

Proximity Dependent Biotin Labelling in Zebrafish for Proteome and Interactome Profiling 用于蛋白质组和相互作用组分析的斑马鱼中的邻近依赖性生物素标记 作者:Zherui Xiong, Harriet P. Lo, Kerrie-Ann McMahon, Robert G. Parton and Thomas E. Hall日期:10/05/2021,浏览量:1527,Q&A: 0

Identification of protein interaction networks is key for understanding intricate biological processes, but mapping such networks is challenging with conventional biochemical methods, especially for weak or transient interactions. Proximity-dependent biotin labelling (BioID) using promiscuous biotin ligases and mass spectrometry (MS)-based

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Transient yet Robust Expression of Proteins in the Mouse Liver via Intravenous Injection of Lipid Nanoparticle-encapsulated Nucleoside-modified mRNA 通过静脉注射脂质纳米颗粒包裹的核苷修饰的 mRNA 蛋白质在小鼠肝脏中瞬时而稳定地表达 作者:Elissa Everton, Fatima Rizvi, Anna R. Smith, Mitchell Beattie, Ying Tam, Norbert Pardi, Drew Weissman and Valerie Gouon-Evans日期:10/05/2021,浏览量:1199,Q&A: 0

With the recent availability of the SARS-CoV-2 mRNA-based vaccines, public attention has been drawn to this new technology and how it may be applied to other indications. Temporal activation of key hepatic regenerative pathways can induce liver regeneration, overcoming the lack of donor organs for liver transplantation and ineffectiveness of

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生物工程

Zebrafish Embryos as a Predictive Animal Model to Study Nanoparticle Behavior in vivo 斑马鱼胚胎作为研究体内纳米粒子行为的预测动物模型 作者:Gabriela Arias-Alpizar, Jeroen Bussmann and Frederick Campbell日期:10/05/2021,浏览量:930,Q&A: 0

A failure to fully understand the complex in vivo behavior of systemically administered nanomedicines has stymied clinical translation. To bridge this knowledge gap, new in vivo tools are needed to rapidly and accurately assess the nearly infinite array of possible nanoparticle designs. Zebrafish embryos are small, transparent, and easily

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癌症生物学

Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice 小鼠白血病细胞代谢的稳定同位素示踪分析 作者:Nick van Gastel, Jessica B. Spinelli, Marcia C. Haigis and David T. Scadden日期:10/05/2021,浏览量:1110,Q&A: 0

Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and targeting metabolic pathways can provide new therapeutic options. Cell metabolism is mostly studied in

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细胞生物学

Isolation of Microvesicles from Human Circulating Neutrophils 人循环中性粒细胞微泡的分离 作者:Dong Zhan, Ellie McConachie, Steven W. Edwards, Helen L. Wright, Robert J. Moots and Sittisak Honsawek日期:10/05/2021,浏览量:1058,Q&A: 0

Neutrophil-derived microvesicles (NDMVs) are liberated by neutrophils upon cell activation by molecules. Once activated, neutrophils are primarily involved in acute inflammation; however, the microvesicles they produce are largely anti-inflammatory. NDMVs have been shown to protect cartilage during inflammatory arthritis. They exert these effects

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Fixation and Immunostaining of Endogenous Proteins or Post-translational Modifications in Caenorhabditis elegans 秀丽隐杆线虫内源性蛋白质的固定和免疫染色或翻译后修饰 作者:Robert O'Hagan and Irini Topalidou日期:10/05/2021,浏览量:1044,Q&A: 0

Although the advent of genetically-encoded fluorescent markers, such as the green fluorescent protein (GFP; Chalfie et al., 1994), has enabled convenient visualization of gene expression in vivo, this method is generally not effective for detecting post-translational modifications because they are not translated from DNA sequences.

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Structural and Functional Mapping of Mesenchymal Bodies 间充质体的结构和功能图谱 作者:Sébastien Sart, Raphaël F.-X. Tomasi, Antoine Barizien, Gabriel Amselem, Ana Cumano and Charles N. Baroud日期:10/05/2021,浏览量:1424,Q&A: 0

The formation of spheroids with mesenchymal stem/stromal cells (MSCs), mesenchymal bodies (MBs), is usually performed using bioreactors or conventional well plates. While these methods promote the formation of a large number of spheroids, they provide limited control over their structure or over the regulation of their environment. It has

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发育生物学

Phalloidin Staining of Actin Filaments for Visualization of Muscle Fibers in Caenorhabditis elegans 秀丽隐杆线虫肌动蛋白丝的Phalloidin染色用于肌纤维可视化 作者:Mario Romani and Johan Auwerx日期:10/05/2021,浏览量:1036,Q&A: 0

Advances in C. elegans research have allowed scientists to recapitulate different human disorders, from neurodegenerative diseases to muscle dysfunction, in these nematodes. Concomitantly, the interest in visualizing organs affected by these conditions has grown, leading to the establishment of different antibody- and dye-based staining protocols

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Isolation of Primary Cytotrophoblasts From Human Placenta at Term 足月人胎盘原代细胞滋养层细胞的分离 作者:Arthur Colson, Christophe Louis Depoix, Corinne Hubinont and Frédéric Debiève日期:10/05/2021,浏览量:537,Q&A: 0

The placenta is a multifaceted organ, fulfilling critical functions for the fetus and the mother. Therefore, it is a critical regulator of the pregnancy, and its dysfunction leads to diseases, including fetal growth restriction and preeclampsia. Studying the placenta is a difficult task since its existence is transient, and its structure is

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免疫学

Calcium Imaging in T Lymphocytes: a Protocol for Use with Genetically Encoded or Chemical Ca2+ Indicators T 淋巴细胞中的钙成像:与遗传编码或化学 Ca2+ 指示一起使用的实验方案 作者:Amit Jairaman and Michael D. Cahalan日期:10/05/2021,浏览量:749,Q&A: 0

Elevations in cytosolic calcium (Ca2+) drive a wide array of immune cell functions, including cytokine production, gene expression, and cell motility. Live-cell imaging of cells loaded with ratiometric chemical Ca2+ indicators remains the gold standard for visualization and quantification of intracellular Ca2+ signals; ratiometric imaging can be

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Measuring Total Classical Pathway and Activities of Individual Components of the Mouse Complement Pathway 测量小鼠补体通路的各个组成部分的总经典通路和活动 作者:Wioleta M. Zelek日期:10/05/2021,浏览量:513,Q&A: 0

The complement system is a central component of innate immunity, responsible for recognition and killing of bacteria by tagging invaders through opsonisation, thereby promoting phagocytosis, and by direct lysis. Complement activity is routinely measured using functional assays that utilise erythrocytes as targets. The classical pathway haemolytic

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微生物学

Simple Methods for the Preparation of Colloidal Chitin, Cell Free Supernatant and Estimation of Laminarinase 胶体几丁质、无细胞上清液的简易制备方法及昆布多糖酶的测定 作者:Ananthamurthy Koteshwara日期:10/05/2021,浏览量:681,Q&A: 0

Colloidal chitin (CC) is a common substrate used in research work involving chitin-active enzymes (chitinases). Cell free supernatant (CFS) is prepared from fermented broth. Preparation of CC and CFS usually involve large amounts of liquid, which must be separated from the solids. This necessitates the use of a large volume centrifugation

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分子生物学

A High-throughput Pipeline to Determine DNA and Nucleosome Conformations by AFM Imaging 通过 AFM 成像确定 DNA 和核小体构象的高通量管道 作者:Sebastian F. Konrad, Willem Vanderlinden and Jan Lipfert日期:10/05/2021,浏览量:691,Q&A: 0

Atomic force microscopy (AFM) is a powerful tool to image macromolecular complexes with nanometer resolution and exquisite single-molecule sensitivity. While AFM imaging is well-established to investigate DNA and nucleoprotein complexes, AFM studies are often limited by small datasets and manual image analysis that is slow and prone to user bias.

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神经科学

Protocols to Induce, Prevent, and Treat Post-traumatic Stress Disorder-like Memory in Mice: Optogenetics and Behavioral Approaches 诱导、预防和治疗小鼠创伤后应激障碍样记忆的方案:光遗传学和行为方法 作者:Aline S. Al Abed, Azza Sellami, Eva-Gunnel Ducourneau, Chloé Bouarab, Aline Marighetto and Aline Desmedt日期:10/05/2021,浏览量:659,Q&A: 0

One of the cardinal features of post-traumatic stress disorder (PTSD) is a paradoxical memory alteration including both emotional hypermnesia for salient trauma-related cues and amnesia for the surrounding traumatic context. Interestingly, some clinical studies have suggested that contextual amnesia would causally contribute to the PTSD-related

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Isolation, Culture, and Identification of Primary Müller Cells from Human Retina 人视网膜原代 Müller 细胞的分离、培养和鉴定 作者:Yingying Chen, Ting Zhang, Shaoxue Zeng, Michelle Yam, Mark C. Gillies and Ling Zhu日期:10/05/2021,浏览量:645,Q&A: 0

Müller cells, the major glial cells of the retina, play vital roles in maintaining redox homeostasis and retinal metabolism. An immortalized human Müller cell line (MIO-M1) is widely used as an in vitro model to study Müller cells’ function, but they may not be exactly the same as primarily cultured human Müller cells. The use of human

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植物科学

Dark Respiration Measurement from Arabidopsis Shoots 拟南芥芽的暗呼吸测量 作者:Jose P. Fonseca, Marcus Griffiths, Larry M. York and Kirankumar S. Mysore日期:10/05/2021,浏览量:942,Q&A: 0

Dark respiration refers to experimental measures of leaf respiration in the absence of light, done to distinguish it from the photorespiration that occurs during photosynthesis. Dark aerobic respiration reactions occur solely in the mitochondria and convert glucose molecules from cytoplasmatic glycolysis and oxygen into carbon dioxide and water,

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