• 卷册 12, 2022
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  • 卷册 2, 2012
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Image Credit: BioProtoc.4441

往期刊物(2022)

卷册: 12 期号: 11

生物化学

H2O2 Release Assay H2O2释放测定 作者:Santosh Yadav, Shruthi Sanjitha Sampath, Brian J. Deskin and Victor J. Thannickal日期:06/05/2022,浏览量:1117,Q&A: 0

Reactive oxygen species are ubiquitous in nature, and function as signalling molecules in biological systems; they may also contribute to oxidative stress in several pathobiological disease states. In this report, we describe a simple, reliable, sensitive, and specific assay for the detection and quantitation of hydrogen peroxide (H2O2) release by

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生物工程

Patterned Substrate of Mobile and Immobile Ligands to Probe EphA2 Receptor Clustering 用于探测 EphA2 受体聚类的移动和固定配体的图案化底物 作者:Zhongwen Chen, Kabir H. Biswas and Jay T. Groves日期:06/05/2022,浏览量:783,Q&A: 0

A multitude of membrane-localized receptors are utilized by cells to integrate both biochemical and physical signals from their microenvironment. The clustering of membrane receptors is widely presumed to have functional consequences for subsequent signal transduction. However, it is experimentally challenging to selectively manipulate receptor

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癌症生物学

Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells 真核细胞的等离子膜损伤和修复试验 作者:Stine Lauritzen Sønder, Malene Laage Ebstrup, Catarina Dias, Anne Sofie Busk Heitmann and Jesper Nylandsted日期:06/05/2022,浏览量:741,Q&A: 0

Damage to the plasma membrane and loss of membrane integrity are detrimental to eukaryotic cells. It is, therefore, essential that cells possess an efficient membrane repair system to survive. However, the different cellular and molecular mechanisms behind plasma membrane repair have not been fully elucidated. Here, we present three complementary

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细胞生物学

Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans 用Argonaute NRDE-3标记活性转录位点—图像秀丽隐杆线虫体内的活性转录位点 作者:Antoine Barrière and Vincent Bertrand日期:06/05/2022,浏览量:522,Q&A: 0

Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat Protein binds, and labels transcription sites and transcripts. Here we describe another strategy, using

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Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice 将小干扰 RNA 电穿孔到小鼠胫骨前肌中 作者:Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis日期:06/05/2022,浏览量:795,Q&A: 0

Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the intramuscular electroporation of DNA plasmids. However, this technique requires surgery and high electrical

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发育生物学

Simple Methods for Permanent or Transient Denervation in Mouse Sciatic Nerve Injury Models 小鼠坐骨神经损伤模型中永久性或暂时性去神经的简单方法 作者:Alexis Osseni, Jean-Luc Thomas, Alireza Ghasemizadeh, Laurent Schaeffer and Vincent Gache日期:06/05/2022,浏览量:613,Q&A: 0

Our ability to move and breathe requires an efficient communication between nerve and muscle that mainly takes place at the neuromuscular junctions (NMJs), a highly specialized synapse that links the axon of a motor neuron to a muscle fiber. When NMJs or axons are disrupted, the control of muscle fiber contraction is lost and muscle are paralyzed.

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Efficient Superovulation and Egg Collection from Mice 高效的超排卵和小鼠卵子收集 作者:Miyuki Shindo, Kenji Miyado, Woojin Kang, Maki Fukami and Mami Miyado日期:06/05/2022,浏览量:566,Q&A: 0

Superovulation is a method used to reduce the number of mice used per experiment by increasing the egg number. Conventionally, superovulation for obtaining mouse eggs involves the use of equine chorionic gonadotropin (eCG) for stimulation and human CG for induction. Female mice of the C57BL/6 inbred strain spontaneously ovulate approximately 10

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Click-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells 在胚胎细胞中Click- iT®Plus OPP Alexa Fluor®蛋白合成分析 作者:Yan Li, Xu Ji, Lu Chang, Jianan Tang, Min-Min Hua, Jing Liu, Christopher O’Neil, Xuefeng Huang and Xingliang Jin日期:06/05/2022,浏览量:611,Q&A: 0

This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT® Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of puromycin, is efficiently incorporated into the nascent polypeptide of newly translated proteins in

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免疫学

Protocol to Isolate Germinal Centers by Laser Microdissection 通过激光显微切割分离生发中心的方法 作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Jürgen Westermann and Kathrin Kalies日期:06/05/2022,浏览量:515,Q&A: 0

During adaptive immune responses, germinal centers (GC) appear as transient microstructures, in which antigen-specific B and T cells interact with each other. Because only the antigen-activated B and T cells, such as Plasmablasts or follicular T helper (Tfh) cells, are present in GC, the in depth-analysis of GC is of great interest. To identify

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Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen 评估小鼠脾脏中造血干细胞和祖细胞的存在 作者:Isabelle J. Marié, Lara Brambilla and David E. Levy日期:06/05/2022,浏览量:654,Q&A: 0

Transplantation of hematopoietic material into recipient mice is an assay routinely used to determine the presence and function of hematopoietic stem and progenitor cells (HSPCs) in vivo. The principle of the method is to transplant donor cells being tested for HSPCs into a recipient mouse following bone marrow ablation and testing for

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Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein 表达红色荧光蛋白的肠沙门氏菌血清鼠伤寒菌体外感染巨噬细胞的流式细胞术研究 作者:Natascha Brigo, Christa Pfeifhofer-Obermair, Egon Demetz, Piotr Tymoszuk and Günter Weiss日期:06/05/2022,浏览量:437,Q&A: 0

Macrophages are important for host defense against intracellular pathogens like Salmonella and can be differentiated into two major subtypes. M1 macrophages, which are pro-inflammatory and induce antimicrobial immune effector mechanisms, including the expression of inducible nitric oxide synthase (iNOS), and M2 macrophages, which exert

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微生物学

A Modified Fluctuation Assay with a CAN1 Reporter in Yeast 酵母中CAN1报告基因的改良波动测定 作者:Pengyao Jiang, Anja R. Ollodart and Maitreya J. Dunham日期:06/05/2022,浏览量:528,Q&A: 0

Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA lines require serial bottlenecks to fix de novo mutations, followed by whole-genome sequencing.

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植物科学

Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation 利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况 作者:Vivek Hari Sundar G. and P. V. Shivaprasad日期:06/05/2022,浏览量:505,Q&A: 0

Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to reliably profile the DNA methylome, especially when considering large genomes such as in crops.

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