生物科学

分类

    现刊
    A Fast and Efficient Decellularization Method for Tissue Slices
    一种快速、高效的组织切片去细胞方法
    [Abstract]

    The study and use of decellularized extracellular matrix (dECM) in tissue engineering, regenerative medicine, and pathophysiology have become more prevalent in recent years. To obtain dECM, numerous decellularization procedures have been developed for the entire organ or tissue blocks, employing either perfusion of decellularizing agents through

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    In vitro Preparation of Homogenous Actin Filaments for Dynamic and Electrophoretic Light Scattering Measurements
    用于动态和电泳光散射测量的均质肌动蛋白丝的体外制备
    作者:Ernesto Alva, Annitta George, Lorenzo Brancaleon and Marcelo Marucho日期:11/20/2022,浏览量:350,Q&A: 0
    [Abstract]

    Actin filaments are essential for various biological activities in eukaryotic cellular processes. Available in vitro experimental data on these systems often lack details and information on sample preparation protocols and experimental techniques, leading to unreproducible results. Additionally, different experimental techniques and polymerization

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    In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay
    体外双泛素形成检测方法和E3协同分析方法
    作者:Rebecca J. Burge, Katie H. Jameson, Anthony J. Wilkinson and Jeremy C. Mottram日期:11/05/2022,浏览量:485,Q&A: 0
    [Abstract]

    Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation. The ubiquitination of substrate proteins typically requires three ubiquitination enzymes: a ubiquitin-activating E1, a

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    First-line Evaluation of Sperm Parameters in Mice (Mus musculus)
    对小鼠(家鼠)精子参数进行一线评估
    作者:Guillaume Martinez日期:10/20/2022,浏览量:581,Q&A: 0
    [Abstract]

    Infertility has become a major public health problem, with a male factor involved in about half the cases. Mice are the most widely used animal model in reproductive biology research laboratories, but changes in sperm parameters in mice can be subtle and, in the absence of official guidelines, it is important that analyses are carried out in a

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    Negative Staining Transmission Electron Microscopy of HIV Viral Particles Permeabilized with PFO and Capsid Stabilized with IP6
    应用透射电子显微镜负染技术观察 PFO 透化的和 IP6 稳定衣壳的 HIV 病毒颗粒
    作者:Derrick Lau, Chantal L. Márquez, Michael W. Parker and Till Böcking日期:10/20/2022,浏览量:622,Q&A: 0
    [Abstract]

    The human immunodeficiency virus 1 (HIV-1) consists of a viral membrane surrounding the conical capsid. The capsid is a protein container assembled from approximately 1,500 copies of the viral capsid protein (CA), functioning as a reaction and transport chamber for the viral genome after cell entry. Transmission electron microscopy (TEM) is a

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    LIST: A Newly Developed Laser-assisted Cell Bioprinting Technology
    LIST:新开发的激光辅助细胞生物打印技术
    作者:Katiane Roversi, Hamid Ebrahimi Orimi, Mahyar Erfanian, Sebastien Talbot and Christos Boutopoulos日期:10/05/2022,浏览量:494,Q&A: 0
    [Abstract]

    Cell bioprinting technologies aim to fabricate tissue-like constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. We recently introduced a laser-assisted drop-on-demand bioprinting technology termed Laser

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    Human Skin Explant Preparation and Culture
    人体皮肤外植体制备和培养
    作者:Jessica L. Shannon, Stephen J. Kirchner and Jennifer Y. Zhang日期:09/20/2022,浏览量:730,Q&A: 0
    [Abstract]

    The ex vivo experimentation with surgically discarded human skin represents a unique methodology amenable for mechanism and pharmacologic agent studies without the involvement of human subjects. Here, we describe a protocol that includes preparation, culture, and stimulation of human skin explants, and subsequent analyses by quantitative reverse

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    Serial Room Temperature Crystal Loading, Data Collection, and Data Processing of Human Glutaminase C in Complex with Allosteric Inhibitors
    人谷氨酰胺酶 C 与变构抑制剂复合物的系列室温晶体加载、数据收集和数据处理
    作者:Shawn K. Milano, D. Marian Szebenyi and Richard A. Cerione日期:09/20/2022,浏览量:508,Q&A: 0
    [Abstract]

    Cancer cells often overexpress glutaminase enzymes, in particular glutaminase C (GAC). GAC resides in the mitochondria and catalyzes the hydrolysis of glutamine to glutamate. High levels of GAC have been observed in aggressive cancers and the inhibition of its enzymatic activity has been shown to reduce their growth and survival. Numerous GAC

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    Combination of Sterile Injury and Microbial Contamination to Model Post-surgical Peritoneal Adhesions in Mice
    结合无菌损伤和微生物污染对小鼠术后腹膜粘连进行建模
    作者:Julia Bayer, Deborah Stroka, Paul Kubes, Daniel Candinas and Joel Zindel日期:08/20/2022,浏览量:1234,Q&A: 0
    [Abstract]

    Abdominal surgeries are frequently associated with the development of post-surgical adhesions. These are irreversible fibrotic scar bands that appear between abdominal organs and the abdominal wall. Patients suffering from adhesions are at risk of severe complications, such as small bowel obstruction, chronic pelvic pain, or infertility. To date,

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    Aerotaxis Assay in Caenorhabditis elegans to Study Behavioral Plasticity
    秀丽隐杆线虫的趋气性测定以研究行为可塑性
    作者:Qiaochu Li, Daniel-Cosmin Marcu, Paul H. Dear and Karl Emanuel Busch日期:08/20/2022,浏览量:966,Q&A: 0
    [Abstract]

    C. elegans shows robust and reproducible behavioral responses to oxygen. Specifically, worms prefer O2 levels of 5–10% and avoid too high or too low O2. Their O2 preference is not fixed but shows plasticity depending on experience, context, or genetic background. We recently showed that this experience-dependent plasticity declines with age,

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