发育生物学

分类

    现刊
    Monitoring the Recruitment and Fusion of Autophagosomes to Phagosomes During the Clearance of Apoptotic Cells in the Nematode Caenorhabditis elegans
    监测秀丽隐杆线虫凋亡细胞清除过程中自噬体与吞噬体的募集与融合
    作者:Omar Peña-Ramos and Zheng Zhou日期:11/20/2022,浏览量:256,Q&A: 0
    [Abstract]

    During an animal's development, a large number of cells undergo apoptosis, a suicidal form of death. These cells are promptly phagocytosed by other cells and degraded inside phagosomes. The recognition, engulfment, and degradation of apoptotic cells is an evolutionarily conserved process occurring in all metazoans. Recently, we discovered a novel

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    In situ Dephosphorylation Assay with Recombinant Nil Phosphatase
    用重组无磷酸酶进行原位去磷酸化测定
    作者:Nilay Nandi, Charles Tracy and Helmut Krämer日期:09/20/2022,浏览量:934,Q&A: 0
    [Abstract]

    The activity of numerous autophagy-related proteins depends on their phosphorylation status, which places importance on understanding the responsible kinases and phosphatases. Great progress has been made in identifying kinases regulating autophagy, but much less is known about the phosphatases counteracting their function. Genetic screens and

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    Preparation of a Single-cell Suspension from Drosophila Wing Imaginal Discs
    果蝇翅成虫芽单细胞悬浮液的制备
    作者:Shu Yang, Brooke Sears and Xiaoyan Zheng日期:08/20/2022,浏览量:1048,Q&A: 0
    [Abstract]

    The wing imaginal discs in Drosophila larvae are a pair of sac-like structures that later form the wings of the adult fly. During the past decades, wing discs have been used as a simple and accessible model system, for identifying genes and deciphering signaling cascades that play crucial roles in many aspects of development. In this protocol, we

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    In vivo Characterization of Endogenous Protein Interactomes in Drosophila Larval Brain, Using a CRISPR/Cas9-based Strategy and BioID-based Proximity Labeling
    利用CRISPR/Cas9 策略和基于BioID的邻近标记研究果蝇幼虫大脑内源性蛋白相互作用体的体内特性
    作者:Ezgi Uçkun, Georg Wolfstetter, Johannes Fuchs and Ruth H. Palmer日期:07/05/2022,浏览量:1658,Q&A: 0
    [Abstract]

    Understanding protein-protein interactions (PPIs) and interactome networks is essential to reveal molecular mechanisms mediating various cellular processes. The most common method to study PPIs in vivo is affinity purification combined with mass spectrometry (AP–MS). Although AP–MS is a powerful method, loss of weak and transient

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    Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
    将小干扰 RNA 电穿孔到小鼠胫骨前肌中
    作者:Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis日期:06/05/2022,浏览量:1317,Q&A: 0
    [Abstract]

    Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the intramuscular electroporation of DNA plasmids. However, this technique requires surgery and high electrical

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    Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle
    从小鼠虹膜角膜角制备单细胞悬液
    作者:Benjamin R. Thomson and Susan E. Quaggin日期:05/20/2022,浏览量:1080,Q&A: 0
    [Abstract]

    Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified novel cell types within the retina, anterior chamber, and iridocorneal angle and explored

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    TUNEL Labeling to Detect Double-stranded DNA Breaks in Caenorhabditis elegans Gonads
    TUNEL 标记检测秀丽隐杆线虫性腺中的双链 DNA 断裂
    作者:Peter A. Kropp, Kyle Rhodehouse and Andy Golden日期:03/20/2022,浏览量:1030,Q&A: 0
    [Abstract]

    Analysis of DNA double strand breaks (DSBs) is important for understanding dyshomeostasis within the nucleus, impaired DNA repair mechanisms, and cell death. In the C. elegans germline, DSBs are important indicators of all three above-mentioned conditions. Although multiple methods exist to assess apoptosis in the germline of C. elegans, direct

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    Combination of Immunofluorescence and Quantitative Fluorescence In-situ Hybridization for Analysing Differential Gene Expression in the Niche Cells of the Drosophila Lymph Gland
    结合免疫荧光和定量荧光原位杂交分析果蝇淋巴腺小细胞中的差异基因表达
    作者:Parvathy Ramesh, Sushmit Ghosh and Lolitika Mandal日期:01/20/2022,浏览量:1816,Q&A: 0
    [Abstract]

    The Drosophila larval haematopoietic organ or lymph gland consists of multiple cell types arranged in zones. The smallest stem cell compartment consists of 40-45 cells that constitute the haematopoietic niche. In order to analyse the haematopoietic niche, it needs to be labelled with a specific antibody to differentiate it from the other cell

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    Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein
    使用探针 2',7'-二氯二氢荧光素测量活细胞中的活性氧和氮种类
    作者:Waleska Dornas and Detlef Schuppan日期:12/20/2021,浏览量:1644,Q&A: 0
    [Abstract]

    Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting

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    Ex-vivo Microtubule Stability Assay Using Drosophila Wing Disc
    利用果蝇翅原基进行体外微管稳定性分析
    作者:Jung-Wan Mok and Kwang-Wook Choi日期:12/05/2021,浏览量:1556,Q&A: 0
    [Abstract]

    Regulation of microtubule stability is crucial for diverse biological processes, including cell division, morphogenesis, and signaling. Various in vitro assays for microtubule stability have been developed to identify and characterize proteins involved in controlling microtubule stability. Here, we introduce a simple ex-vivo assay for identifying

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