生物科学

分类

    现刊
    In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay
    体外双泛素形成检测方法和E3协同分析方法
    作者:Rebecca J. Burge, Katie H. Jameson, Anthony J. Wilkinson and Jeremy C. Mottram日期:11/05/2022,浏览量:485,Q&A: 0
    [Abstract]

    Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation. The ubiquitination of substrate proteins typically requires three ubiquitination enzymes: a ubiquitin-activating E1, a

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    Negative Staining Transmission Electron Microscopy of HIV Viral Particles Permeabilized with PFO and Capsid Stabilized with IP6
    应用透射电子显微镜负染技术观察 PFO 透化的和 IP6 稳定衣壳的 HIV 病毒颗粒
    作者:Derrick Lau, Chantal L. Márquez, Michael W. Parker and Till Böcking日期:10/20/2022,浏览量:622,Q&A: 0
    [Abstract]

    The human immunodeficiency virus 1 (HIV-1) consists of a viral membrane surrounding the conical capsid. The capsid is a protein container assembled from approximately 1,500 copies of the viral capsid protein (CA), functioning as a reaction and transport chamber for the viral genome after cell entry. Transmission electron microscopy (TEM) is a

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    Cryptococcus neoformans Virulence Assay Using a Galleria mellonella Larvae Model System
    用大蜡螟幼虫模型系统进行新型隐球菌毒力测定
    作者:Piotr R. Stempinski, Daniel F. Q. Smith and Arturo Casadevall日期:08/05/2022,浏览量:1000,Q&A: 0
    [Abstract]

    Cryptococcus neoformans is a human pathogenic fungus that can cause pulmonary infections and meningitis in both immunocompromised and otherwise healthy individuals. Limited treatment options and a high mortality rate underlie the necessity for extensive research of the virulence of C. neoformans. Here we describe a detailed protocol for using the

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    Analysis of Caenorhabditis elegans Aging-related Neurodegeneration in Chemosensory Neurons
    秀丽隐杆线虫化学感觉神经元衰老相关神经退行性变分析
    作者:Cira Crespo and Roberto Grau日期:07/20/2022,浏览量:1073,Q&A: 0
    [Abstract]

    Aging and neuronal deterioration constitute important risk factors for the development of neuronal-related diseases, such as different dementia. The nematode Caenorhabditis elegans has emerged as a popular model system for studying neurodegeneration diseases, due to its complete neuronal connectivity map. DiI is a red fluorescent dye that can

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    A Modified Fluctuation Assay with a CAN1 Reporter in Yeast
    酵母中CAN1报告基因的改良波动测定
    作者:Pengyao Jiang, Anja R. Ollodart and Maitreya J. Dunham日期:06/05/2022,浏览量:1017,Q&A: 0
    [Abstract]

    Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA lines require serial bottlenecks to fix de novo mutations, followed by whole-genome sequencing.

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    Rhizoctonia solani Infection Assay of Young Sugar Beet and Arabidopsis plantlets
    甜菜和拟南芥幼苗丝核枯菌侵染试验
    作者:Fredrik Dölfors, Louise Holmquist, Georgios Tzelepis and Christina Dixelius日期:01/20/2022,浏览量:1924,Q&A: 0
    [Abstract]

    Rhizoctonia solani is a soil-borne fungus, which rarely produces any spores in culture. Hence, all inoculation procedures are based on mycelia, often as a coat on cereal kernels, placed in close vicinity to the plant to be infected. In this protocol, an inoculation method is described where the fungus is first allowed to infest a perlite-maize

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    A Retro-orbital Sinus Injection Mouse Model to Study Early Events and Reorganization of the Astrocytic Network during Pneumococcal Meningitis
    用于研究肺炎球菌脑膜炎期间星形胶质细胞网络的早期事件和重组的眶后窦注射小鼠模型
    作者:Chakir Bello, Martine Cohen-Salmon and Guy Tran Van Nhieu日期:12/05/2021,浏览量:1324,Q&A: 0
    [Abstract]

    Pneumococcal (PN) meningitis is a life-threatening disease with high mortality rates that leads to permanent neurological sequelae. Studies of the process of bacterial crossing of the blood brain barrier (BBB) are hampered by the lack of relevant in vitro and in vivo models of meningitis that recapitulate the human disease. PN meningitis involves

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    Quantification of RuBisCO Expression and Photosynthetic Oxygen Evolution in Cyanobacteria
    蓝藻RuBisCO表达和光合氧释放的定量研究
    作者:Mateusz Kędzior and Betul Kacar日期:10/20/2021,浏览量:1474,Q&A: 0
    [Abstract]

    Phototrophic microorganisms are frequently engineered to regulate the expression and the activity of targeted enzymes of interest for specific biotechnological and agricultural applications. This protocol describes a method to evaluate the expression of RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) in the model cyanobacterium

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    Intrathoracic Inoculation of Zika Virus in Aedes aegypti
    埃及伊蚊胸内接种寨卡病毒
    作者:Irma Sanchez-Vargas, Adeline E. Williams, Alexander W.E. Franz and Ken E. Olson日期:09/20/2021,浏览量:1575,Q&A: 0
    [Abstract]

    Aedes aegypti mosquitoes are the main vectors of many medically relevant arthropod-borne (arbo) viruses, including Zika (ZIKV), dengue (DENV), and yellow fever (YFV). Vector competence studies with Ae. aegypti often involve challenging mosquitoes with an artificial bloodmeal containing virus and later quantifying viral titer or infectious

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    Isolation and Characterization of Membrane Vesicles from Lactobacillus Species
    乳酸杆菌属膜囊泡中的分离和特性研究
    作者:Julie C. Caruana, Scott N. Dean and Scott A. Walper日期:09/05/2021,浏览量:2390,Q&A: 0
    [Abstract]

    Throughout their life cycle, bacteria shed portions of their outermost membrane comprised of proteins, lipids, and a diversity of other biomolecules. These biological nanoparticles have been shown to have a range of highly diverse biological activities, including pathogenesis, community regulation, and cellular defense (among others). In recent

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