XZ
Xueliang Zhu
  • State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, China
研究方向
  • Developmental biology
Purification and Immunostaining of Mouse Ependymal Ciliary Shafts
小鼠室管膜纤毛干的纯化和免疫染色
作者:Kai Hao, Xueliang Zhu and Xiumin Yan日期:07/20/2022,浏览量:1874,Q&A: 0

Cilia and flagella are microtubule-based hair-like organelles protruding from the surface of most eukaryotic cells, and play essential roles in cell locomotion, left-right asymmetry, embryo development, and tissue homeostasis. With isolated cilia and flagella, great progress has been made in understanding the composition, structure, and function of cilia. However, the current cilia/flagella isolation methods are deficient in the integrity or productivity of purified cilia when applied to mammalian motile cilia. Here, we describe a new protocol that isolates cilia shafts from mouse ependymal cells, by horizontal shear force and mild detergent. This method enables the production of virtually integral cilia with high yields and less cell body contamination. It is suitable for immunostaining, puromycin labeling assay, and proximity ligation assay of mammalian motile cilia.


Graphical abstract:




Fluorescence Microscopy for Cilia in Cultured Cells and Zebrafish Embryos
荧光显微镜法检测培养细胞和斑马鱼胚胎中的纤毛
作者:Jingli Cao, Xueliang Zhu and Xiumin Yan日期:07/20/2014,浏览量:14346,Q&A: 0
Cilia are microtubule-based hair-like projections found in organisms, ranging from protozoa to mammals. This protocol provides methods for immunofluorescence staining of cilia in cultured cells and zebrafish embryos.