联合作者(
Kentaro Kajiwara Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Japan
1 protocol

Takefumi Karashima Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan
1 protocol

Kouichi Funato
  • Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan
研究方向
  • 微生物学
  • 已发表 protocol 1

Education

Ph.D. in Graduate School of Pharmaceutical Sciences, University of Tokushima, 1994

Current position

Associate Professor in Graduate School of Biosphere Sciences, Hiroshima University

Publications

  1. Kajiwara, K., Muneoka, T., Watanabe, Y., Karashima, T., Kitagaki, H. and Funato, K. (2012). Perturbation of sphingolipid metabolism induces endoplasmic reticulum stress-mediated mitochondrial apoptosis in budding yeast. Mol Microbiol 86(5): 1246-1261.
  2. Kajiwara, K., Watanabe, R., Pichler, H., Ihara, K., Murakami, S., Riezman, H. and Funato, K. (2008). Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum. Mol Biol Cell 19(5): 2069-2082.
  3. Funato, K. Riezman, H. (2006). Part 2-8, Sphingolipid Trafficking, HIRABAYASHI, Yoshio, IGARASHI, Yasuyuki, MERRILL, Alfred H. Jr, (Eds.), Sphingolipid Biology, pp.123-139, Springer.
  4. Funato, K., Lombardi, R., Vallee, B. and Riezman, H. (2003). Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae. J Biol Chem 278(9): 7325-7334.
  5. Funato, K., Vallee, B. and Riezman, H. (2002). Biosynthesis and trafficking of sphingolipids in the yeast Saccharomyces cerevisiae. Biochemistry 41(51): 15105-15114.
  6. Funato, K. and Riezman, H. (2001). Vesicular and nonvesicular transport of ceramide from ER to the Golgi apparatus in yeast. J Cell Biol 155(6): 949-959.
已发表 protocol 1篇
Metabolic Labeling of Yeast Sphingolipids with Radioactive D-erythro-[4,5-3H]dihydrosphingosine
使用放射性D-赤氏-[4,5-3氢]二氢神经鞘氨醇对酵母鞘脂类进行代谢标记

作者:Takefumi Karashima, Kentaro Kajiwara and Kouichi Funato日期:08/20/2013,浏览量:9304,Q&A: 0
Yeast sphingolipids can be metabolically labeled with exogenously added radioactive precursors. Here we describe a method to label ceramides and complex sphingolipids of Saccharomyces cerevisiae with a radioactive ceramide precursor, ...