联合作者(
Wolf B. Frommer Heinrich Heine University Düsseldorf
4 protocols

Guido Grossmann Centre for Organismal Studies, Heidelberg University , Germany
2 protocols

Roberto De Michele CNR-IGV Institute of Biosciences and Bioresources
1 protocol
联合评审(
Arsalan Daudi University of California
157 protocols

Fanglian He Bio-protocol
205 protocols

Feng Li Huazhong Agricultural University
17 protocols

Igor Cesarino University of São Paulo
9 protocols

Cindy Ast Cooke
  • Post-Doc, Carnegie Institution for Science
研究方向
  • 生物物理学
  • Fluorescent Proteins, Biosensors, Spectroscopy, Microscopy
  • 已发表 protocol 1

教育背景

PhD, University of Potsdam

实验室信息

Frommer Lab

发表论文

Ast, C., Foret, J., Oltrogge, L.M., De Michele, R., Kleist, T.J., Ho, C.-H., and Frommer, W.B. Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun (2017).
Ast, C., De Michele, R., Kumke, M. U. & Frommer, W. B. Single-fluorophore membrane transport activity sensors with dual-emission read-out. eLife 4, (2015).
Ast, C., Frommer, W. B., Grossmann, G. & De Michele, R. Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors. Bio-Protoc. 5, e1372 (2015).
Ast, C. & Draaijer, A. in Low-Oxygen Stress in Plants (eds. Dongen, J. T. van & Licausi, F.) 397–417 (Springer Vienna, 2014).
Haupt, J. et al. ACVR1 p.Q207E causes classic fibrodysplasia ossificans progressiva and is functionally distinct from the engineered constitutively active ACVR1 p.Q207D variant. Hum. Mol. Genet. 23, 5364–5377 (2014).
De Michele, R. et al. Fluorescent sensors reporting the activity of ammonium transceptors in live cells. eLife 2, e00800 (2013).
Wang, Q. et al. Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization. Proc. Natl. Acad. Sci. U. S. A. 110, 13204–13209 (2013).
Ast, C., Schmälzlin, E., Löhmannsröben, H.-G. & van Dongen, J. T. Optical oxygen micro- and nanosensors for plant applications. Sensors 12, 7015–7032 (2012).
Hill, D. et al. Size determination of quantum dots with fluorescence correlation spectroscopy. in (eds. Parak, W. J., Yamamoto, K. & Osinski, M.) 790904 (2011). doi:10.1117/12.874660
Shen, Q. et al. The fibrodysplasia ossificans progressiva R206H ACVR1 mutation activates BMP-independent chondrogenesis and zebrafish embryo ventralization. J. Clin. Invest. 119, 3462–3472 (2009).
已发表 protocol 1篇
Quantification of Extracellular Ammonium Concentrations and Transporter Activity in Yeast Using AmTrac Fluorescent Sensors
采用AmTrac荧光传感器定量测定酵母细胞的胞外铵浓度和转运蛋白活性

作者:Cindy Ast, Wolf B. Frommer, Guido Grossmann and Roberto De Michele日期:01/05/2015,浏览量:9776,Q&A: 0
AmTracs are the first example of “activity sensors”, since they report the activity of ammonium transporters by means of fluorescence readout in vivo (De Michele et al., 2013). AmTracs are based on a single fluorescent protein, a ...
参审 protocol 11篇
Hydroponic Culture of ‘Micro-Tom’ Tomato
水培法培养‘Micro-Tom’ 番茄
作者:Reiko Motohashi, Harumi Enoki, Chikako Fukazawa and Yoshikazu Kiriiwa日期:10/05/2015,浏览量:12057,Q&A: 3
We use ‘Micro-Tom’ to study tomato fruit ripening and development mechanisms. ‘Micro-Tom’ is suitable for cultivation and experiments due to its small size of 10 to 20 cm in height and short life cycle of 3 months. There is also an abundance of ...
查看全文 >
Protein Degradation Assays in Arabidopsis Protoplasts
利用拟南芥原生质体系统检测蛋白质的降解
Plant transformation and exogenous protein expression is essential for molecular biology and biotechnology. Current approaches of stable plant transformation might be problematic and very time-consuming. Because of this, transient expression in ...
查看全文 >