Education

Ph.D. in Molecular Biology, University of Konstanz, Germany, 1992

Current position

Tenured Scientist, Departamento de Biología del Estrés y Patología, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Murcia, Spain

Publications

1. Miras, M., Sempere, R. N., Kraft, J. J., Miller, W. A., Aranda, M. A. and Truniger, V. (2014). Interfamilial recombination between viruses led to acquisition of a novel translation-enhancing RNA element that allows resistance breaking. New Phytol 202(1): 233-246.
2. Juarez, M., Legua, P., Mengual, C. M., Kassem, M. A., Sempere, R. N., Gómez, P., Truniger, V., and Aranda, M. A. (2013). Relative incidence, spatial distribution and genetic diversity of cucurbit viruses in eastern Spain. Ann Appl Biol 162(3): 362-370.
3. Rodriguez-Hernandez, A. M., Gosalvez, B., Sempere, R. N., Burgos, L., Aranda, M. A. and Truniger, V. (2012). Melon RNA interference (RNAi) lines silenced for Cm-eIF4E show broad virus resistance. Mol Plant Pathol 13(7): 755-763.
4. Gonzalez-Ibeas, D., Blanca, J., Cañizares, J., Blanca, V., Truniger and Aranda, M. A. (2012). A cost-effective double-stranded cDNA synthesis for plant microarrays. Plant Mol Biol Rep 30(5): 1276-1282.
5. Clepet, C., Joobeur, T., Zheng, Y., Jublot, D., Huang, M., Truniger, V., Boualem, A., Hernandez-Gonzalez, M. E., Dolcet-Sanjuan, R., Portnoy, V., Mascarell-Creus, A., Cano-Delgado, A. I., Katzir, N., Bendahmane, A., Giovannoni, J. J., Aranda, M. A., Garcia-Mas, J. and Fei, Z. (2011). Analysis of expressed sequence tags generated from full-length enriched cDNA libraries of melon. BMC Genomics 12: 252.
6. Sempere, R. N., Gomez, P., Truniger, V. and Aranda, M. A. (2011). Development of expression vectors based on pepino mosaic virus. Plant Methods 7: 6.
7. Nieto, C., Rodriguez-Moreno, L., Rodriguez-Hernandez, A. M., Aranda, M. A. and Truniger, V. (2011). Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E. Plant J 66(3): 492-501.
8. Truniger, V., Aranda, M. A. (2009). Recessive resistance to plant viruses. Adv Virus Res 75: 119-231.
9. Truniger, V., Nieto, C., Gonzalez-Ibeas, D. and Aranda, M. (2008). Mechanism of plant eIF4E-mediated resistance against a Carmovirus (Tombusviridae): cap-independent translation of a viral RNA controlled in cis by an (a)virulence determinant. Plant J 56(5): 716-727.
10. Gonzalez-Ibeas, D., Blanca, J., Roig, C., Gonzalez-To, M., Pico, B., Truniger, V., Gomez, P., Deleu, W., Cano-Delgado, A., Arus, P., Nuez, F., Garcia-Mas, J., Puigdomenech, P. and Aranda, M. A. (2007). MELOGEN: an EST database for melon functional genomics. BMC Genomics 8: 306.
11. Kassem, M. A., Sempere, R. N., Juárez, M., Aranda, M. A. and Truniger, V. (2007). Cucurbit aphid-borne yellows virus is prevalent in field-grown cucurbit crops of southeastern Spain. Plant Disease 91(3): 232-238.
12. Nieto, C., Piron, F., Dalmais, M., Marco, C. F., Moriones, E., Gomez-Guillamon, M. L., Truniger, V., Gomez, P., Garcia-Mas, J., Aranda, M. A. and Bendahmane, A. (2007). EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility. BMC Plant Biol 7: 34.
13. Trunige, V., Kassem, M. and Aranda, M. A. (2006). Cucurbit yellow stunting disorder virus (CYSDV). In: Rao, G., Kumar, L. and Holguín-Peña, R. (eds). Characterization, diagnosis and manegement of plant viruses. Studium Press, 283-301.
14. Truniger, V., Bonnin, A., Lazaro, J. M., de Vega, M. and Salas, M. (2005). Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding. Gene 348: 89-99.
15. Juarez, M., Truniger, V. and Aranda, M. A. (2004). First report of Cucurbit aphid-borne yellows virus in Spain. Plant Disease 88(8): 907-907.
16. Truniger, V., Nieto, C., Juan A. D. and Aranda, M. A. (2004). A Melon necrotic spot virus untranslated RNA sequence is the genetic avirulence determinant for both cultivar and non-host resistances. Int Proc. 2nd European Congress of Virology 7-10. 
17. Diaz-Pendon, J. A., Truniger, V., Nieto, C., Garcia-Mas, J., Bendahmane, A. and Aranda, M. A. (2004). Advances in understanding recessive resistance to plant viruses. Mol Plant Pathol 5(3): 223-233.
18. Diaz, J. A., Nieto, C., Moriones, E., Truniger, V. and Aranda, M. A. (2004). Molecular characterization of a Melon necrotic spot virus strain that overcomes the resistance in melon and nonhost plants. Mol Plant Microbe Interact 17(6): 668-675.
19. Truniger, V., Lázaro, J. M.  and Salas, M. (2004). Two positively charged residues of ø 29 DNA polymerase, conserved in protein-primed DNA polymerases are involved in stabilization of the incoming nucleotide. J Mol Biol 335: 481-494.
20. Truniger, V., Lazaro, J. M. and Salas, M. (2004). Function of the C-terminus of phi29 DNA polymerase in DNA and terminal protein binding. Nucleic Acids Res 32(1): 361-370.
21. Truniger, V., Lázaro, J. M., de Vega, M., Blanco, L. and Salas, M. (2003). ϕ29 DNA polymerase residue Leu384, highly conserved in motif B of eukaryotic type DNA replicases, is involved in nucleotide insertion fidelity. J Biol Chem 278(35): 33482-33491.
22. Aguilar, J. M., Franco, M., Marco, C. F., Berdiales, B., Rodriguez-Cerezo, E., Truniger, V. and Aranda, M. A. (2003). Further variability within the genus Crinivirus, as revealed by determination of the complete RNA genome sequence of Cucurbit yellow stunting disorder virus. J Gen Virol 84(Pt 9): 2555-2564.
23. Truniger, V., Lazaro, J. M., Esteban, F. J., Blanco, L. and Salas, M. (2002). A positively charged residue of phi29 DNA polymerase, highly conserved in DNA polymerases from families A and B, is involved in binding the incoming nucleotide. Nucleic Acids Res 30(7): 1483-1492.
24. Truniger, V., Lazaro, J. M., Blanco, L. and Salas, M. (2002). A highly conserved lysine residue in phi29 DNA polymerase is important for correct binding of the templating nucleotide during initiation of phi29 DNA replication. J Mol Biol 318(1): 83-96.
25. Brenkman, A. B., Heideman, M. R., Truniger, V., Salas, M. and van der Vliet, P. C. (2001). The (I/Y)XGG motif of adenovirus DNA polymerase affects template DNA binding and the transition from initiation to elongation. J Biol Chem 276(32): 29846-29853.   
    
26. Truniger, V., Blanco, L. and Salas, M. (2000). Analysis of O29 DNA polymerase by partial proteolysis: binding of terminal protein in the double-stranded DNA channel. J Mol Biol 295(3): 441-453.
27. Truniger, V., Blanco, L. and Salas, M. (1999). Role of the "YxGG/A" motif of Phi29 DNA polymerase in protein-primed replication. J Mol Biol 286(1): 57-69.
28. Truniger, V., Lazaro, J. M., Salas, M. and Blanco, L. (1998). Phi 29 DNA polymerase requires the N-terminal domain to bind terminal protein and DNA primer substrates. J Mol Biol 278(4): 741-755.
29. Truniger, V., Lazaro, J. M., Salas, M. and Blanco, L. (1996). A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases. EMBO J 15(13): 3430-3441.
30. Truniger, V. and Boos, W. (1994). Mapping and cloning of gldA, the structural gene of the Escherichia coli glycerol dehydrogenase. J Bacteriol 176(6): 1796-1800.
31. Truniger, V. and Boos, W. (1993). Glycerol uptake in Escherichia coli is sensitive to membrane lipid composition. Res Microbiol 144(7): 565-574.
32. Truniger, V., Boos, W. and Sweet, G. (1992). Molecular analysis of the glpFKX regions of Escherichia coli and Shigella flexneri. J Bacteriol 174(21): 6981-6991.
33. Sweet, G., Gandor, C., Voegele, R., Wittekindt, N., Beuerle, J., Truniger, V., Lin, E. C. and Boos, W. (1990). Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product. J Bacteriol 172(1): 424-430.