Denise Scuffi, Instituto de Investigaciones Biológicas, Universidad Nacional Mar del Plata, Consejo Científicas y Técnicas (IIB-UNMdP-CONICET), Argentina

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Denise Scuffi

Research Associate, Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (IIB-UNMdP-CONICET), Argentina

研究方向

Biochemistry, Cell Biology, Molecular Biology, Plant Science

个人信息

教育背景

Dr, Universidad Nacional de Mar del Plata, 2017

实验室信息

Signalling Mechanisms in Plants Lab ahead by Dr. Ana M. Laxalt
The group's research lines focus on the study of lipid second messengers, gasotransmitters and the regulation of ion channels as intermediaries in signal transduction processes in plants, among others. These processes are studied, mainly, in two experimental systems:

Plant-pathogen interaction: involves the role of the different isoenzymes of phospholipase C in the perception of pathogens and the induction of defence responses, to contribute to a deeper knowledge of the resistance/susceptibility mechanisms of plants; and
The regulation of stomatal pore: refers to the role of hydrogen sulfur (H2S). This is a low molecular weight gas, belonging to the group of gas transmitters. This line focuses on the role of H2S in the signalling pathways that regulate stomatal closure in response to different stimuli.
https://iib.conicet.gov.ar/mecanismos-de-senalizacion-en-plantas/

研究方向

We studied stomata regulation upon different stimuli through microscopy (fluorescence and optical), biosensors, molecular biology and biochemistry approximation.

发表论文

2023. Pantaleno R., Scuffi D*. Post-translational modifications triggered by H2S in plant cells. In: Modolo L. V. and Da-Silva, C.J. (eds) Plant Gasotransmitters and Molecules with Hormonal Activity. H2S in Plants
Past, Present and Beyond, El Sevier. ISBN: 978-0-323-99035-6. *Corresponding author

2022. Pantaleno, R; Scuffi, D; Costa, A, Welchen, E, Torregrossa, R, Whiteman, M and García-Mata, C. “The mitochondrial H2S donor AP39 induces stomatal closure through the modulation of guard cell’s mitochondrial activity”. Accepted for publication in Plant Physiology. DOI: 10.1093/plphys/kiac591

2021. Pantaleno, R; Scuffi, D; García-Mata, C. “Hydrogen sulphide as a guard cell network regulator”. New phytol. 230: 451-456.

2021. Scuffi D., García-Mata C. Hydrogen Sulfide and Stomatal Movement. In: Khan M.N., Siddiqui M.H., Alamri S., Corpas F.J. (eds) Hydrogen Sulfide and Plant Acclimation to Abiotic Stresses. Plant in Challenging Environments, vol 1. Springer, Cham. https://doi.org/10.1007/978-3-030-73678-1_6

2018. van Wijk, R; Zhang, Q; Zarza, X; Lamers, M; Reyes Marquez, F; Guardia, A; Scuffi, D; García-Mata, C; Ligterink, W; Haring, M. A; Laxalt, A. M; Munnik, T. “Role for Arabidopsis PLC7 in Stomatal Movement, Seed Mucilage Attachment, and Leaf Serration”. Front. Plant Sci. 9:1721. DOI: 10.3389/fpls.2018.01721

2018. Zhang, Q; van Wijk, R; Zarza, X; Shahbaz, M; van Hooren, M; Guardia, A; Scuffi, D; García-Mata, C; Van den Ende, W; Hoffmann-Benning, S; Haring, M. A; Laxalt, A. M; Munnik, T. “Knock-Down of Arabidopsis PLC5 Reduces Primary Root Growth and Secondary Root Formation While Overexpression Improves Drought Tolerance and Causes Stunted Root Hair Growth”. Plant Cell Physiol. 0(0): 1–16 doi:10.1093/pcp/pcy120

2018. Scuffi, D; Nietzel, T; Di Fino, L; Meyer, A.J; Lamattina, L; Schwarzländer, M; Laxalt, A.M; García-Mata, C. “Hydrogen sulfide increases production of NADPH oxidase-dependent hydrogen peroxide and phospholipase D-derived phosphatidic acid in guard cell signaling”. Plant Physiol. 176: 2532–2542

2017. Zhang Q; van Wijk R, Shahbaz M, Roels W, van Schooten B, Vermeer JEM, Zarza X, Guardia A, Scuffi D, García-Mata C, Laha D, Williams P, Willems LAJ, Ligterink W, Hoffmann-Benning S, Gillaspy G, Schaaf G, Haring MA, Laxalt AM, Munnik T. "Arabidopsis Phospholipase C3 is Involved in Lateral Root Initiation and ABA Responses in Seed Germination and Stomatal Closure". Plant Cell Physiol. DOI: 10.1093/pcp/pcx194

2017. D'Ambrosio JM, Couto D, Fabro G, Scuffi D, Lamattina L, Munnik T, Andersson M, Alvarez M, Zipfel C; Laxalt A. M. "PLC2 Regulates MAMP-Triggered Immunity by Modulating ROS Production in Arabidopsis". Plant Physiol. 175: 970–981

2016. Scuffi D, Lamattina L; García-Mata C. Gasotransmitters and stomatal closure: is there redundancy, concerted action, or both? Front. Plant Sci. 7:277. DOI: 10.3389/fpls.2016.00277

2016. Scuffi D, Lamattina L and García-Mata C. “Decoding the Interaction between Nitric Oxide and Hydrogen Sulfide in Stomatal Movement”. Gasotransmitters in Plants, Springer. ISBN 978-3-319-40713-5

2015. Distéfano, A.M., Valiñas, M.A., Scuffi, D., Lamattina, L., ten Have, A., García-Mata, C., Laxalt, A.M. Phospholipase D δ knock-out mutants are tolerant to severe drought stress. Plant Signaling and Behavior. DOI: 10.1080/15592324.2015.1089371.

2015. Papanatsiou, M., Scuffi, D., Blatt, R.M., García-Mata, C. Hydrogen sulphide regulates inward-rectifying K+ channels in conjunction with stomatal closure. Plant Physiol. 168: 29-35.

2015. Foresi, N., Mayta, M., Lodeyro, A., Scuffi, D., Correa-Aragunde, N., García-Mata, C., Casalongué, C., Carrillo, N; Lamattina, L. Expression of the tetrahydrofolate-dependent nitric oxide synthase from the green alga Ostreococcus tauri increases tolerance to abiotic stresses and influences stomatal development in Arabidopsis. P Journal. 82: 806-821.

2014. Scuffi, D., Álvarez, C., Laspina, N., Gotor, C,, Lamattina, L; García-Mata, C. Hydrogen sulfide generated by L-cysteine desulfhydrase acts upstream of nitric oxide to modulate ABA-dependent stomatal closure. Plant Physiol. 166: 2065-2076

2012. Distéfano, A.M; Scuffi, D; García-Mata, C; Lamattina, L; Laxalt, A.M. Phospholipase Dδ is involved in nitric oxide-induced stomatal closure. Planta. 236: 1899-907