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联合作者(
)
Rachael Marie Giersch
Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
1 protocol
Jeremy Thorner
University of California, Berkeley
1 protocol
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联合评审(
)
Joëlle Schläpfer
University of Zurich
13 protocols
Melike Çağlayan
University of Florida
4 protocols
Ralph Thomas Boettcher
Max Planck Institute for Biochemistry
54 protocols
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评审
Gregory C. Finnigan
Faculty, Kansas State University
研究方向
分子生物学
贡献
已发表 protocol
2
篇
参审 protocol
篇
主审 protocol
篇
教育背景
University of Oregon
实验室信息
https://www.k-state.edu/bmb/department/directory/finnigan/lab/
已发表 protocol 2篇
Method for Multiplexing CRISPR/Cas9 in
Saccharomyces cerevisiae
Using Artificial Target DNA Sequences
酿酒酵母中使用人工靶DNA序列进行多重CRISPR/ Cas9的方法
作者:
Rachael M. Giersch
and
Gregory C. Finnigan
,
日期:
09/20/2017,
浏览量:
10319,
Q&A:
0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
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Complex
in vivo
Ligation Using Homologous Recombination and High-efficiency Plasmid Rescue from
Saccharomyces cerevisiae
通过同源重组进行复合体的体内连接及在酿酒酵母中进行高效质粒拯救
作者:
Gregory C. Finnigan
and
Jeremy Thorner
,
日期:
07/05/2015,
浏览量:
11543,
Q&A:
0
The protocols presented here allow for the facile generation of a wide variety of complex multipart DNA constructs (tagged gene products, gene fusions, chimeric proteins, and other variants) using homologous recombination and
in vivo
...
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参审 protocol 2篇
Trypsin Sensitivity Assay to Study the Folding Status of Proteins
胰蛋白酶敏感性试验研究蛋白质的折叠状态
作者:
Satoshi Ninagawa
and
Kazutoshi Mori
,
日期:
10/05/2016,
浏览量:
9461,
Q&A:
0
This protocol aims to evaluate folding status of proteins, utilizing trypsin sensitivity. Unfolded/misfolded proteins are more susceptible to trypsin than folded proteins, because trypsin easily accesses and cleaves loosely folded parts of proteins. ...
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PNGase Sensitivity Assay to Study the Folding Status of Proteins
聚糖酶敏感性试验研究蛋白质的折叠状态
作者:
Satoshi Ninagawa
and
Kazutoshi Mori
,
日期:
10/05/2016,
浏览量:
6828,
Q&A:
0
This protocol aims to evaluate folding status of proteins, utilizing peptide:N-glycanase (PNGase) sensitivity. In the cytosol, PNGase works as a deglycosylation-enzyme. N-glycans on unfolded/misfolded proteins are more susceptible to PNGase than ...
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