Kyohei Higashi
  • Graduate School of Pharmaceutical Sciences, Chiba University, Japan, Japan,
  • Biochemistry


Ph.D in pharmaceutical sciences, Chiba University, Japan, 2006

Current position

Assistant Professor, Graduate School of Pharmaceutical Sciences , Chiba University, Japan

Publications (since 2013)

  1. Higashi, K., Shibasaki, M., Kuni, K., Uemura, T., Waragai, M., Uemura, K., Igarashi, K. and Toida, T. (2017). Determination of 3-hydroxypropylmercapturic acid in urine by three column-switching high-performance liquid chromatography with electrochemical detection using a diamond electrode. J Chromatogr A 1517: 79-85.
  2. Yamada, T., Kerever, A., Yoshimura, Y., Suzuki, Y., Nonaka, R., Higashi, K., Toida, T., Mercier, F. and Arikawa-Hirasawa, E. (2017). Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor-2 signaling impairment in the aged neurogenic niche. J Neurochem.
  3. Sugiyama, Y., Nakamura, A., Matsumoto, M., Kanbe, A., Sakanaka, M., Higashi, K., Igarashi, K., Katayama, T., Suzuki, H. and Kurihara, S. (2016). A Novel Putrescine Exporter SapBCDF of Escherichia coli. J Biol Chem 291(51): 26343-26351.
  4. Higashi, K., Takeda, K., Mukuno, A., Okamoto, Y., Masuko, S., Linhardt, R. J. and Toida, T. (2016). Identification of keratan sulfate disaccharide at C-3 position of glucuronate of chondroitin sulfate from Mactra chinensis. Biochem J 473(22): 4145-4158.
  5. Imamura, M., Higashi, K., Yamaguchi, K., Asakura, K., Furihata, T., Terui, Y., Satake, T., Maegawa, J., Yasumura, K., Ibuki, A., Akase, T., Nishimura, K., Kashiwagi, K., Linhardt, R. J., Igarashi, K. and Toida, T. (2016). Polyamines release the let-7b-mediated suppression of initiation codon recognition during the protein synthesis of EXT2. Sci Rep 6: 33549.
  6. Higashi, K., Igarashi, K. and Toida, T. (2016). Recent Progress in Analytical Methods for Determination of Urinary 3-Hydroxypropylmercapturic Acid, a Major Metabolite of Acrolein. Biol Pharm Bull 39(6): 915-919.
  7. Sugiyama, S., Ishikawa, S., Tomitori, H., Niiyama, M., Hirose, M., Miyazaki, Y., Higashi, K., Murata, M., Adachi, H., Takano, K., Murakami, S., Inoue, T., Mori, Y., Kashiwagi, K., Igarashi, K. and Matsumura, H. (2016). Molecular mechanism underlying promiscuous polyamine recognition by spermidine acetyltransferase. Int J Biochem Cell Biol 76: 87-97.
  8. Ge, D., Higashi, K., Ito, D., Nagano, K., Ishikawa, R., Terui, Y., Higashi, K., Moribe, K., Linhardt, R. J. and Toida, T. (2016). Poly-ion Complex of Chondroitin Sulfate and Spermine and Its Effect on Oral Chondroitin Sulfate Bioavailability. Chem Pharm Bull (Tokyo) 64(5): 390-398.
  9. Tamai, N., Akase, T., Minematsu, T., Higashi, K., Toida, T., Igarashi, K. and Sanada, H. (2016). Association Between Components of Exudates and Periwound Moisture-Associated Dermatitis in Breast Cancer Patients With Malignant Fungating Wounds. Biol Res Nurs 18(2): 199-206.
  10. Higashi, K., Okamoto, Y., Mukuno, A., Wakai, J., Hosoyama, S., Linhardt, R. J. and Toida, T. (2015). Functional chondroitin sulfate from Enteroctopus dofleini containing a 3-O-sulfo glucuronic acid residue. Carbohydr Polym 134: 557-565.
  11. Hirose, T., Saiki, R., Yoshizawa, Y., Imamura, M., Higashi, K., Ishii, I., Toida, T., Williams, K., Kashiwagi, K. and Igarashi, K. (2015). Spermidine and Ca2+, but not Na+, can permeate NMDA receptors consisting of GluN1 and GluN2A or GluN2B in the presence of Mg2+. Biochem Biophys Res Commun 463: 1190-1195 .
  12. Suabjakyong, P., Saiki, R., Van Griensven, L. J., Higashi, K., Nishimura, K., Igarashi, K. and Toida, T. (2015). Polyphenol extract from Phellinus igniarius protects against acrolein toxicity in vitro and provides protection in a mouse stroke model. PLoS One 10(3): e0122733.
  13. Higashi, K., Takeuchi, Y., Mukuno, A., Tomitori, H., Miya, M., Linhardt, R. J. and Toida, T. (2015). Composition of glycosaminoglycans in elasmobranchs including several deep-sea sharks: identification of chondroitin/dermatan sulfate from the dried fins of Isurus oxyrinchus and Prionace glauca. PLoS One 10(3): e0120860.
  14. Akiyama, H., Matsuoka, H., Okuyama, T., Higashi, K., Toida, T., Komatsu, H., Sugita-Konishi, Y., Kobori, S., Kodama, Y., Yoshida, M. and Endou, H. (2015). The acute encephalopathy induced by intake of sugihiratake mushroom in the patients with renal damage might be associated with the intoxication of cyanide and thiocyanate. Food Safety 3: 16-29.
  15. Yoshida, M., Higashi, K., Kuni, K., Mizoi, M., Saiki, R., Nakamura, M., Waragai, M., Uemura, K., Toida, T., Kashiwagi, K. and Igarashi, K. (2015). Distinguishing mild cognitive impairment from Alzheimer's disease with acrolein metabolites and creatinine in urine. Clin Chim Acta 441: 115-121.
  16. Terui, Y., Sakamoto, A., Yoshida, T., Kasahara, T., Tomitori, H., Higashi, K., Igarashi, K. and Kashiwagi, K. (2015). Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA. Amino Acids 47(2): 345-356.
  17. Higashi, K., Okamoto, Y., Mano, T., Wada, T. and Toida, T. (2014). A simple HPLC method for identification of the origin of chondroitin sulfate in health foods. Jpn J Food Chem 21: 187-194.
  18. Higashi, K., Imamura, M., Fudo, S., Uemura, T., Saiki, R., Hoshino, T., Toida, T., Kashiwagi, K. and Igarashi, K. (2014). Identification of functional amino acid residues involved in polyamine and agmatine transport by human organic cation transporter 2. PLoS One 9(7): e102234.
  19. Terui, Y., Saroj, S. D., Sakamoto, A., Yoshida, T., Higashi, K., Kurihara, S., Suzuki, H., Toida, T., Kashiwagi, K. and Igarashi, K. (2014). Properties of putrescine uptake by PotFGHI and PuuP and their physiological significance in Escherichia coli. Amino Acids 46(3): 661-670.
  20. Hayashi, Y., Sugiyama, H., Suganami, A., Higashi, K., Kashiwagi, K., Igarashi, K., Kawauchi, S. and Tamura, Y. (2013). Prediction of the interaction between spermidine and the G-G mismatch containing acceptor stem in tRNA(Ile): molecular modeling, density functional theory, and molecular dynamics study. Biochem Biophys Res Commun 441(4): 999-1004.
  21. Niiyama, M., Sugiyama, S., Hirose, M., Ishikawa, S., Tomitori, H., Higashi, K., Yamashita, T., Adachi, H., Takano, K., Murakami, S., Murata, M., Inoue, T., Mori, Y., Kashiwagi, K., Matsumura, H. and Igarashi, K. (2013). Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun 69(Pt 8): 884-887.
  22. Uemura, T., Tanaka, Y., Higashi, K., Miyamori, D., Takasaka, T., Nagano, T., Toida, T., Yoshimoto, K., Igarashi, K. and Ikegaya, H. (2013). Acetaldehyde-induced cytotoxicity involves induction of spermine oxidase at the transcriptional level. Toxicology 310: 1-7.
  23. Yamashita, T., Nishimura, K., Saiki, R., Okudaira, H., Tome, M., Higashi, K., Nakamura, M., Terui, Y., Fujiwara, K., Kashiwagi, K. and Igarashi, K. (2013). Role of polyamines at the G1/S boundary and G2/M phase of the cell cycle. Int J Biochem Cell Biol 45(6): 1042-1050.
  24. Igarashi, N., Takeguchi, A., Sakai, S., Akiyama, H., Higashi, K. and Toida, T. (2013). Effect of molecular sizes of chondroitin sulfate on interaction with L-Selectin. Int J Carbohydr Chem.
  25. Zhao, X., Yang, B., Solakyildirim, K., Joo, E. J., Toida, T., Higashi, K., Linhardt, R. J. and Li, L. (2013). Sequence analysis and domain motifs in the porcine skin decorin glycosaminoglycan chain. J Biol Chem 288(13): 9226-9237.