Education
Ph.D. in Biochemistry, Department of Biochemistry, University of Dundee, Scotland, U.K., 1985
Current position
Professor in General Microbiology, Institute of Biology, Martin-Luther University Halle-Wittenberg, Halle, Saale, Germany
Publications (since 2010)
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Jaroschinsky, M., and Sawers, R. G. (2014). Ferredoxin has a pivotal role in the biosynthesis of the hydrogen-oxidizing hydrogenases in Escherichia coli. Int. J. Hydrogen Energy. (In press).
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Pinske, C. and Sawers, R. G. (2014). The importance of iron in the biosynthesis and assembly of [NiFe]-hydrogenases. BioMolecular Concepts 5(1): 55-70.
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Sawers, R. G., Hunger, D. and Doberenza, C. (2014). Identification of key residues in the formate channel FocA that control import and export of formate. Biol Chem. (Epub ahead of print)
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Doberenz, C., Zorn, M., Falke, D., Nannemann, D., Hunger, D., Beyer, L., Ihling, C. H., Meiler, J., Sinz, A. and Sawers, R. G. (2014). Pyruvate Formate-Lyase Interacts Directly with the Formate Channel FocA to Regulate Formate Translocation. J Mol Biol. (Epub ahead of print)
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Trchounian, A. and Gary Sawers, R. (2013). Novel insights into the bioenergetics of mixed‐acid fermentation: Can hydrogen and proton cycles combine to help maintain a proton motive force? IUBMB Life.
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Sawers, R. G. (2013). Nickel in Bacteria and Archaea. Encyclopedia of Metalloproteins: 1490-1496
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Soboh, B. and Sawers, R. G. (2013). [NiFe]-hydrogenase cofactor assembly. Encyclopedia of Inorganic and Bioorganic Chemistry. Metals in Cells, Chapter eibc2154 (In press)
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Brauer, L., Schicht, M., Worlitzsch, D., Bensel, T., Sawers, R. G. and Paulsen, F. (2013). Staphylococcus aureus and Pseudomonas aeruginosa express and secrete human surfactant proteins. PLoS One 8(1): e53705.
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Beyer, L., Doberenz, C., Falke, D., Hunger, D., Suppmann, B. and Sawers, R. G. (2013). Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products. J Bacteriol 195(7): 1428-1435.
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Trchounian, K., Soboh, B., Sawers, R. G. and Trchounian, A. (2013). Contribution of hydrogenase 2 to stationary phase H2 production by Escherichia coli during fermentation of glycerol. Cell Biochem Biophys 66(1): 103-108.
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Wagner, A., Segler, L., Kleinsteuber, S., Sawers, G., Smidt, H. and Lechner, U. (2013). Regulation of reductive dehalogenase gene transcription in Dehalococcoides mccartyi. Philos Trans R Soc Lond B Biol Sci 368(1616): 20120317.
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Zorn, M., Ihling, C. H., Golbik, R., Sawers, R. G. and Sinz, A. (2013). Selective selC-independent selenocysteine incorporation into formate dehydrogenases. PLoS One 8(4): e61913.
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Pinske, C., Jaroschinsky, M. and Sawers, R. G. (2013). Levels of control exerted by the Isc iron-sulfur cluster system on biosynthesis of the formate hydrogenlyase complex. Microbiology 159(Pt 6): 1179-1189.
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Stripp, S. T., Soboh, B., Lindenstrauss, U., Braussemann, M., Herzberg, M., Nies, D. H., Sawers, R. G. and Heberle, J. (2013). HypD is the scaffold protein for Fe-(CN)2CO cofactor assembly in [NiFe]-hydrogenase maturation. Biochemistry 52(19): 3289-3296.
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Fischer, M. and Sawers, R. G. (2013). A universally applicable and rapid method for measuring the growth of streptomyces and other filamentous microorganisms by methylene blue adsorption-desorption. Appl Environ Microbiol 79(14): 4499-4502.
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Soboh, B., Stripp, S. T., Bielak, C., Lindenstrauss, U., Braussemann, M., Javaid, M., Hallensleben, M., Granich, C., Herzberg, M., Heberle, J. and Sawers, R. G. (2013). The [NiFe]-hydrogenase accessory chaperones HypC and HybG of Escherichia coli are iron- and carbon dioxide-binding proteins. FEBS Lett 587(16): 2512-2516.
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Fischer, M., Falke, D. and Sawers, R. G. (2013). A respiratory nitrate reductase active exclusively in resting spores of the obligate aerobe Streptomyces coelicolor A3(2). Mol Microbiol 89(6): 1259-1273.
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Poladyan, A., Trchounian, K., Sawers, R. G. and Trchounian, A. (2013). Hydrogen-oxidizing hydrogenases 1 and 2 of Escherichia coli regulate the onset of hydrogen evolution and ATPase activity, respectively, during glucose fermentation at alkaline pH. FEMS Microbiol Lett 348(2): 143-148.
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Sawers R.G. (2012) Hydrogenase maturation endopeptidase. Chapter 70. In: Rawlings, N. and Salvesen, G. (eds.) Handbook of Proteolytic Enzymes. 3rd Edition. Academic Press.
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Pinske, C. and Sawers, R. G. (2012). A-type carrier protein ErpA is essential for formation of an active formate-nitrate respiratory pathway in Escherichia coli K-12. J Bacteriol 194(2): 346-353.
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Pinske, C., McDowall, J. S., Sargent, F. and Sawers, R. G. (2012). Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12. Microbiology 158(Pt 3): 856-868.
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Pinske, C. and Sawers, R. G. (2012). Delivery of iron-sulfur clusters to the hydrogen-oxidizing [NiFe]-hydrogenases in Escherichia coli requires the A-type carrier proteins ErpA and IscA. PLoS One 7(2): e31755.
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Trchounian, K., Pinske, C., Sawers, R. G. and Trchounian, A. (2012). Characterization of Escherichia coli [NiFe]-hydrogenase distribution during fermentative growth at different pHs. Cell Biochem Biophys 62(3): 433-440.
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Kirchberg, J., Buttner, D., Thiemer, B. and Sawers, R. G. (2012). Aconitase B is required for optimal growth of Xanthomonas campestris pv. vesicatoria in pepper plants. PLoS One 7(4): e34941.
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Fischer, M., Schmidt, C., Falke, D. and Sawers, R. G. (2012). Terminal reduction reactions of nitrate and sulfate assimilation in Streptomyces coelicolor A3(2): identification of genes encoding nitrite and sulfite reductases. Res Microbiol 163(5): 340-348.
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Soboh, B., Kuhns, M., Braussemann, M., Waclawek, M., Muhr, E., Pierik, A. J. and Sawers, R. G. (2012). Evidence for an oxygen-sensitive iron-sulfur cluster in an immature large subunit species of Escherichia coli [NiFe]-hydrogenase 2. Biochem Biophys Res Commun 424(1): 158-163.
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Pinske, C., Jaroschinsky, M., Sargent, F. and Sawers, G. (2012). Zymographic differentiation of [NiFe]-hydrogenases 1, 2 and 3 of Escherichia coli K-12. BMC Microbiol 12: 134.
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Soboh, B., Stripp, S. T., Muhr, E., Granich, C., Braussemann, M., Herzberg, M., Heberle, J. and Gary Sawers, R. (2012). [NiFe]-hydrogenase maturation: isolation of a HypC-HypD complex carrying diatomic CO and CN- ligands. FEBS Lett 586(21): 3882-3887.
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Pinske, C., Kruger, S., Soboh, B., Ihling, C., Kuhns, M., Braussemann, M., Jaroschinsky, M., Sauer, C., Sargent, F., Sinz, A. and Sawers, R. G. (2011). Efficient electron transfer from hydrogen to benzyl viologen by the [NiFe]-hydrogenases of Escherichia coli is dependent on the coexpression of the iron-sulfur cluster-containing small subunit. Arch Microbiol 193(12): 893-903.
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Pinske, C., Bonn, M., Kruger, S., Lindenstrauss, U. and Sawers, R. G. (2011). Metabolic deficiences revealed in the biotechnologically important model bacterium Escherichia coli BL21(DE3). PLoS One 6(8): e22830.
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Soboh, B., Pinske, C., Kuhns, M., Waclawek, M., Ihling, C., Trchounian, K., Trchounian, A., Sinz, A. and Sawers, G. (2011). The respiratory molybdo-selenoprotein formate dehydrogenases of Escherichia coli have hydrogen: benzyl viologen oxidoreductase activity. BMC Microbiol 11: 173.
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Pinske, C. and Sawers, G. (2011). Iron restriction induces preferential down-regulation of H(2)-consuming over H(2)-evolving reactions during fermentative growth of Escherichia coli. BMC Microbiol 11: 196.
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Petkun, S., Shi, R., Li, Y., Asinas, A., Munger, C., Zhang, L., Waclawek, M., Soboh, B., Sawers, R. G. and Cygler, M. (2011). Structure of hydrogenase maturation protein HypF with reaction intermediates shows two active sites. Structure 19(12): 1773-1783.
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Trchounian, K., Pinske, C., Sawers, R. G. and Trchounian, A. (2011). Dependence on the F0F1-ATP synthase for the activities of the hydrogen-oxidizing hydrogenases 1 and 2 during glucose and glycerol fermentation at high and low pH in Escherichia coli. J Bioenerg Biomembr 43(6): 645-650.
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Bekker, M., Alexeeva, S., Laan, W., Sawers, G., Teixeira de Mattos, J. and Hellingwerf, K. (2010). The ArcBA two-component system of Escherichia coli is regulated by the redox state of both the ubiquinone and the menaquinone pool. J Bacteriol 192(3): 746-754.
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Falke, D., Schulz, K., Doberenz, C., Beyer, L., Lilie, H., Thiemer, B. and Sawers, R. G. (2010). Unexpected oligomeric structure of the FocA formate channel of Escherichia coli : a paradigm for the formate-nitrite transporter family of integral membrane proteins. FEMS Microbiol Lett 303(1): 69-75.
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Harris, R. L., Barbieri, S., Paraskevopoulos, K., Murphy, L. M., Eady, R. R., Hasnain, S. S. and Sawers, R. G. (2010). Characterization of cycP gene expression in Achromobacter xylosoxidans NCIMB 11015 and high-level heterologous synthesis of cytochrome c' in Escherichia coli. J Mol Microbiol Biotechnol 18(2): 102-108.
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Pinske, C. and Gary Sawers, R. (2010). The role of the ferric-uptake regulator Fur and iron homeostasis in controlling levels of the [NiFe]-hydrogenases in Escherichia coli. International Journal of Hydrogen Energy 35(17): 8938-8944.
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Fischer, M., Alderson, J., van Keulen, G., White, J. and Sawers, R. G. (2010). The obligate aerobe Streptomyces coelicolor A3(2) synthesizes three active respiratory nitrate reductases. Microbiology 156(Pt 10): 3166-3179.
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Soboh, B., Kruger, S., Kuhns, M., Pinske, C., Lehmann, A. and Sawers, R. G. (2010). Development of a cell-free system reveals an oxygen-labile step in the maturation of [NiFe]-hydrogenase 2 of Escherichia coli. FEBS Lett 584(18): 4109-4114.