• 卷册 11, 2021
  • 卷册 10, 2020
  • 卷册 9, 2019
  • 卷册 8, 2018
  • 卷册 7, 2017
  • 卷册 6, 2016
  • 卷册 5, 2015
  • 卷册 4, 2014
  • 卷册 3, 2013
  • 卷册 2, 2012
  • 卷册 1, 2011
Image Credit: BioProtoc.4017

往期刊物(2021)

卷册: 11 期号: 10

生物化学

Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins 有多重二硫化物键的正确折叠蛋白质的无细胞合成:真菌疏水性化合物的生产 作者:Rezwan Siddiquee and Ann H Kwan日期:05/20/2021,浏览量:1144,Q&A: 0

Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using Escherichia coli lysate (Figure 1) to successfully express a class of proteins (known as hydrophobins)

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Intracellular IRF5 Dimerization Assay 细胞内IRF5二聚分析 作者:Cherrie D. Sherman and Betsy J. Barnes日期:05/20/2021,浏览量:913,Q&A: 0

The intracellular interferon regulatory factor 5 (IRF5) dimerization assay is a technique designed to measure molecular interaction(s) with endogenous IRF5. Here, we present two methods that detect endogenous IRF5 homodimerization and interaction of endogenous IR5 with cell penetrating peptide (CPP) inhibitors. Briefly, to detect endogenous IRF5

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A Fluorescence Dequenching-based Liposome Leakage Assay to Measure Membrane Permeabilization by Pore-forming Proteins 一种以测定成孔蛋白膜通透性的基于荧光淬灭的脂质体渗漏测定方法 作者:Javier Aguilera, Salvador Vazquez-Reyes and Jianjun Sun日期:05/20/2021,浏览量:867,Q&A: 0

Pore-forming toxins (PFTs) have been discovered in a wide range of organisms. Their functions are essential to the survival or virulence of many species. PFTs often interact with lipid membranes. Large unilamellar vesicles (LUV), also known as liposomes, have been commonly used as reliable membrane models for testing PFTs activity. Liposomes have

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生物物理学

Single-Molecule Studies of Membrane Receptors from Brain Region Specific Nanovesicles 脑区特异性纳米泡膜受体的单分子研究 作者:Surya P. Aryal, Xu Fu, Abdullah A. Masud, Khaga R. Neupane and Christopher I. Richards日期:05/20/2021,浏览量:914,Q&A: 0

Single molecule imaging and spectroscopy are powerful techniques for the study of a wide range of biological processes including protein assembly and trafficking. However, in vivo single molecule imaging of biomolecules has been challenging because of difficulties associated with sample preparation and technical challenges associated with

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癌症生物学

Surface Engineering and Multimodal Imaging of Multistage Delivery Vectors in Metastatic Breast Cancer 转移性乳腺癌多期转运载体的表面工程和多模态成像 作者:Shreya Goel, Mauro Ferrari and Haifa Shen日期:05/20/2021,浏览量:804,Q&A: 0

The design of effective nanoformulations that target metastatic breast cancers is challenging due to a lack of competent imaging and image analysis protocols that can capture the interactions between the injected nanoparticles and metastatic lesions. Here, we describe the integration of in vivo whole-body PET-CT with high temporal resolution, ex

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细胞生物学

FACS Enrichment of Total Interstitial Cells and Fibroblasts from Adult Mouse Ventricles 流式细胞术富集成年小鼠心室间质细胞和成纤维细胞 作者:Nona Farbehi, Vaibhao Janbandhu, Robert E. Nordon and Richard P. Harvey日期:05/20/2021,浏览量:966,Q&A: 0

Besides cardiomyocytes, the heart contains numerous interstitial cell types, including cardiac fibroblasts, endothelial cells, immune (myeloid and lymphoid) cells, and mural cells (pericytes and vascular smooth muscle cells), which play key roles in heart repair, regeneration, and disease. We recently published a comprehensive map of cardiac

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发育生物学

Imaging and Fluorescence Quantification in Caenorhabditis elegans with Flow Vermimetry and Automated Microscopy 秀丽隐杆线虫的流动蠕虫仪和自动显微镜成像和荧光定量研究 作者:Elissa Tjahjono, Alexey V. Revtovich and Natalia V. Kirienko日期:05/20/2021,浏览量:674,Q&A: 0

Gene activation and cellular biomarkers are commonly monitored using fluorescent signals from transgenic reporters or dyes. These quantifiable markers are critical for biological research and serve as an incredibly powerful tool, even more so when combined with high-throughput screening. Caenorhabditis elegans is a particularly useful model in

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免疫学

In vitro and In vivo CD8+ T Cell Suppression Assays CD8+ T细胞体外和体内抑制试验 作者:Lu Xie, Guihuan Liu, Yanjun Liu and Yuming Yu日期:05/20/2021,浏览量:1001,Q&A: 0

CD8+CD28− T suppressor cells (Ts) have been documented to promote immune tolerance by suppressing effector T cell responses to alloantigens following transplantation. The suppressive function of T cells has been defined as the inhibitory effect of Ts on the proliferation rate of effector T cells. 3H-thymidine is a classical immunological

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Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening 从2003年和2019年SARS冠状病毒和四种常见人类冠状病毒的血清学分析和抑制剂筛选中产生病毒刺突蛋白受体结合域 作者:Bruno Segovia-Chumbez, Stephen D. Graham, Ramesh Jadi, Aravinda M. de Silva and Lakshmanane Premkumar日期:05/20/2021,浏览量:860,Q&A: 0

The recombinant receptor-binding domain (RBD) of the viral spike protein from SARS-CoV-1 and 2 are reliable antigens for detecting viral-specific antibodies in humans. We and others have shown that the levels of RBD-binding antibodies and SARS-CoV-2 neutralizing antibodies in patients are correlated. Here, we report the expression and purification

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A Simple and Robust Protocol for in vitro Differentiation of Mouse Non-pathogenic T Helper 17 Cells from CD4+ T Cells 一种简单稳定的用小鼠CD4+ T细胞体外分化非致病性T辅助17细胞的方法 作者:Siwen Kang, Ruohan Wu and Ruoning Wang日期:05/20/2021,浏览量:735,Q&A: 0

Functional and mechanistic studies of CD4+ T cell lineages rely on robust methods of in vitro T cell polarization. Here, we report an optimized protocol for in vitro differentiation of a mouse non-pathogenic T helper 17 (TH17) cell lineage. Most of the previously established protocols require irradiated splenocytes as artificial antigen presenting

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微生物学

Parasitemia Evaluation in Mice Infected with Schistosoma mansoni 曼氏血吸虫感染小鼠的寄生虫病评估 作者:Naiara Clemente Tavares and Marina Moraes Mourão日期:05/20/2021,浏览量:487,Q&A: 0

Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma

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ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates ODELAM:结核分枝杆菌耐药性的快速序列独立检测 作者:Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison日期:05/20/2021,浏览量:477,Q&A: 0

Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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Tracking the Subcellular Localization of Surface Proteins in Staphylococcus aureus by Immunofluorescence Microscopy 用免疫荧光显微镜追踪金黄色葡萄球菌表面蛋白的亚细胞定位 作者:Salvatore J. Scaffidi, Mac A. Shebes and Wenqi Yu日期:05/20/2021,浏览量:1279,Q&A: 0

Surface proteins of Staphylococcus aureus and other Gram-positive bacteria play essential roles in bacterial colonization and host-microbe interactions. Surface protein precursors containing a YSIRK/GXXS signal peptide are translocated across the septal membrane at mid-cell, anchored to the cell wall peptidoglycan at the cross-wall compartment,

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分子生物学

A New Method for Studying RNA-binding Proteins on Specific RNAs 一种研究特异性RNA结合蛋白的新方法 作者:Weiping Sun, Ziheng Zhang, Ji-Long Liu and Min Zhuang日期:05/20/2021,浏览量:1362,Q&A: 0

Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by combining the RNA-binding capacity of CRISPR/Cas13 and the proximity-tagging activity of PUP-IT.

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In vivo CD40 Silencing by siRNA Infusion in Rodents and Evaluation by Kidney Immunostaining 小鼠体内siRNA转染CD40沉默和肾免疫染色评价 作者:Miguel Hueso, Adrián Mallén, Elia Ripoll, Laura de Ramón, Nuria Bolaños, Cristian Varela, Jordi Guiteras, Javier Checa, Estanislao Navarro, Josep Maria Grinyo, Josep Maria Cruzado, Josep Maria Aran and Joan Torras日期:05/20/2021,浏览量:647,Q&A: 0

The co-stimulatory molecule CD40 and its ligand CD40L play a key role in the regulation of immunological processes and are involved in the pathophysiology of autoimmune and inflammatory diseases. Inhibition of the CD40-CD40L axis is a promising therapy, and a number of strategies and techniques have been designed to hinder its functionality. Our

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神经科学

Operant Vapor Self-administration in Mice 小鼠蒸汽吸入自我给药操作 作者:Renata C. N. Marchette, Brendan J. Tunstall, Leandro F. Vendruscolo and Khaled Moussawi日期:05/20/2021,浏览量:615,Q&A: 0

Models of drug addiction in rodents are instrumental in understanding the underlying neurobiology. Intravenous self-administration of drugs in mice is currently the most commonly used model; however, several challenges exist due to complications related to catheter patency. To take full advantage of the genetic tools available to study opioid

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干细胞

Ex vivo Tissue Culture Protocols for Studying the Developing Neocortex 研究发育中的新皮质的体外组织培养方案 作者:Takashi Namba, Christiane Haffner and Wieland B. Huttner日期:05/20/2021,浏览量:593,Q&A: 0

The size of the neocortex and its morphology are highly divergent across mammalian species. Several approaches have been utilized for the analysis of neocortical development and comparison among different species. In the present protocol (Note: This protocol requires basic knowledge of brain anatomy), we describe three ex vivo neocortical

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