神经科学

分类

    现刊
    3D-printed Recoverable Microdrive and Base Plate System for Rodent Electrophysiology
    用于啮齿动物电生理学的3D打印可恢复微驱动器和基板系统
    [Abstract]

    Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations of neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed in recent years (e.g., µLED and Neuropixels silicon probes), implantation methods for silicon probes in

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    Isolation of Nuclei from Mouse Dorsal Root Ganglia for Single-nucleus Genomics
    小鼠背根神经节细胞核的分离及其单核基因组学研究
    作者:Lite Yang, Ivan Tochitsky, Clifford J. Woolf and William Renthal日期:08/05/2021,浏览量:692,Q&A: 0
    [Abstract]

    Primary somatosensory neurons, whose cell bodies reside in the dorsal root ganglion (DRG) and trigeminal ganglion, are specialized to transmit sensory information from the periphery to the central nervous system. Our molecular understanding of peripheral sensory neurons has been limited by both their heterogeneity and low abundance compared with

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    CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes
    基于选择性表达基因的CRISPR介导的鸡胚胎细胞标记
    作者:Masahito Yamagata and Joshua R. Sanes日期:08/05/2021,浏览量:1224,Q&A: 0
    [Abstract]

    The abilities to mark and manipulate specific cell types are essential for an increasing number of functional, structural, molecular, and developmental analyses in model organisms. In a few species, this can be accomplished by germline transgenesis; in other species, other methods are needed to selectively label somatic cells based on the genes

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    Cell-attached and Whole-cell Patch-clamp Recordings of Dopamine Neurons in the Substantia Nigra Pars Compacta of Mouse Brain Slices
    小鼠脑片黑质致密部多巴胺神经元的细胞贴附和全细胞膜片钳记录
    作者:Stefano Cattaneo, Maria Regoni, Jenny Sassone and Stefano Taverna日期:08/05/2021,浏览量:769,Q&A: 0
    [Abstract]

    The Substantia Nigra pars compacta (SNc) is a midbrain dopaminergic nucleus that plays a key role in modulating motor and cognitive functions. It is crucially involved in several disorders, particularly Parkinson’s disease, which is characterized by a progressive loss of SNc dopaminergic cells. Electrophysiological studies on SNc neurons are

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    Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts
    膜片钳移液管重复使用的快速酶清洗方法:通过消除膜片钳尝试之间的手动移液管更换来提高吞吐量
    作者:Corey R. Landry, Mighten C. Yip, Ilya Kolb, William A. Stoy, Mercedes M. Gonzalez and Craig R. Forest日期:07/20/2021,浏览量:863,Q&A: 0
    [Abstract]

    The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and automation were limited by the need to replace pipettes manually after each experiment. This

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    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    神经元的电生理特性:小鼠脑切片中的电流钳记录和放电模式分析
    作者:Elina Nagaeva, Ivan Zubarev and Esa R. Korpi日期:06/20/2021,浏览量:1450,Q&A: 0
    [Abstract]

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain are tremendously diverse in their membrane characteristics and abilities to generate action

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    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:1689,Q&A: 0
    [Abstract]

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the

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    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    同种人类中脑类器官的生成与维持
    作者:Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder日期:06/05/2021,浏览量:2073,Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

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    Single-Molecule Studies of Membrane Receptors from Brain Region Specific Nanovesicles
    脑区特异性纳米泡膜受体的单分子研究
    作者:Surya P. Aryal, Xu Fu, Abdullah A. Masud, Khaga R. Neupane and Christopher I. Richards日期:05/20/2021,浏览量:2158,Q&A: 0
    [Abstract]

    Single molecule imaging and spectroscopy are powerful techniques for the study of a wide range of biological processes including protein assembly and trafficking. However, in vivo single molecule imaging of biomolecules has been challenging because of difficulties associated with sample preparation and technical challenges associated with

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    Transfection and Activation of CofActor, a Light and Stress Gated Optogenetic Tool, in Primary Hippocampal Neuron Cultures
    一种在原代海马神经元培养能辅助因子的转染和激活的光和应激门控的光遗传学工具
    作者:Wyatt P. Bunner, Rachel Dodson, Robert M. Hughes and Erzsebet M. Szatmari日期:04/20/2021,浏览量:1074,Q&A: 0
    [Abstract]

    Proteins involved in neurodegeneration can be coupled with optogenetic reagents to create rapid and sensitive reporters to provide insight into the biochemical processes that mediate the progression of neurodegenerative disorders, including Alzheimer’s Disease (AD). We have recently developed a novel optically-responsive tool (the

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