系统生物学

分类

    现刊
    Transient Expression Assay in NahG Arabidopsis Plants Using Agrobacterium tumefaciens
    使用根癌农杆菌在NahG拟南芥植株中进行瞬时表达分析
    作者:Pepe Cana-Quijada, Eduardo R. Bejarano, Rosa Lozano-Durán and Tábata Rosas-Díaz日期:06/20/2018,浏览量:6357,Q&A: 1
    [Abstract] Agrobacterium-mediated transient expression has greatly contributed to research in molecular plant biology but has low efficiency and inconsistency in Arabidopsis thaliana (Arabidopsis). Here, we describe a simple, efficient and fast protocol to make transient gene expression in NahG Arabidopsis plants using ...
    Synthetic Genetic Interaction (CRISPR-SGI) Profiling in Caenorhabditis elegans
    秀丽隐杆线虫中合成遗传相互作用(CRISPR-SGI)分析
    作者:John A. Calarco and Adam D. Norris日期:03/05/2018,浏览量:5920,Q&A: 0
    [Abstract] Genetic interaction screens are a powerful methodology to establish novel roles for genes and elucidate functional connections between genes. Such studies have been performed to great effect in single-cell organisms such as yeast and E. coli (Schuldiner et al., 2005; Butland et al., 2008; Costanzo et al., 2010), ...
    Protocol for Molecular Dynamics Simulations of Proteins
    蛋白质的分子动力学模拟方案
    作者:MNV Prasad Gajula, Anuj Kumar and Johny Ijaq日期:12/05/2016,浏览量:20201,Q&A: 0
    [Abstract] Molecular dynamics (MD) simulations have become one of the most important tools in understanding the behavior of bio-molecules on nanosecond to microsecond time scales. In this protocol, we provide a general approach and standard setup protocol for MD simulations by using the Gromacs MD suite.
    Computational Identification of MicroRNA-targeted Nucleotide-binding Site-leucine-rich Repeat Genes in Plants
    植物中microRNA靶向结合的富含亮氨酸重复序列基因的计算识别
    作者:Zhu-Qing Shao, Yan-Mei Zhang, Bin Wang and Jian-Qun Chen日期:11/05/2015,浏览量:8063,Q&A: 0
    [Abstract] Plant genomes harbor dozens to hundreds of nucleotide-binding site-leucine-rich repeat (NBS-LRR, NBS for short) type disease resistance genes (Shao et al., 2014; Zhang et al., 2015). Proper regulation of these genes is important for normal growth of plants by reducing unnecessary fitness costs in the absence of pathogen ...
    Character-State Reconstruction to Infer Ancestral Protein-Protein Interaction Patterns
    特征状态重建法推断遗传蛋白质相互作用模式
    作者:Florian Rümpler, Günter Theißen and Rainer Melzer日期:08/20/2015,浏览量:10735,Q&A: 0
    [Abstract] Protein-protein interactions are at the core of a plethora of developmental, physiological and biochemical processes. Consequently, insights into the origin and evolutionary dynamics of protein-protein interactions may provide information on the constraints and dynamics of specific biomolecular circuits and their impact on the organismal ...
    A Conceptual Outline for Omics Experiments Using Bioinformatics Analogies
    采用生物信息类推法进行组学实验概念概述
    作者:Prashanth Suravajhala and Jeffrey W. Bizzaro日期:02/05/2015,浏览量:9913,Q&A: 0
    [Abstract] Hypothetical proteins (HP) are those that are not characterized in the laboratory and so remain “orphaned” in genomic databases. In recent times there has been a lot of progress in characterizing HPs in the laboratory. Various methods, such as sequence capture and Next Generation Sequencing (NGS), have been used to rapidly identify HP functions ...
    SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions
    基于拟南芥滞绿报告基因(SGR)分析植物转录因子与启动子之间的相互作用
    作者:Shisong Ma and Savithramma Dinesh-Kumar日期:08/20/2014,浏览量:8891,Q&A: 0
    [Abstract] We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants. The system uses the Arabidopsis stay green (SGR) gene as a reporter. Induction of SGR expression in N. ...
    Gene Networks Based on the Graphical Gaussian Model
    高斯图模型构建基因网络
    作者:Shisong Ma日期:02/20/2012,浏览量:13184,Q&A: 1
    [Abstract] This protocol describes how to build a gene network based on the graphical Gaussian model (GGM) from large scale microarray data. GGM uses partial correlation coefficient (pcor) to infer co-expression relationship between genes. Compared to the traditional Pearson’ correlation coefficient, partial correlation is a better measurement of direct ...