细胞生物学

分类

    现刊
    Modeling the Nonlinear Dynamics of Intracellular Signaling Networks
    细胞内信号网络的非线性动力学建模
    作者:Oleksii S. Rukhlenko and Boris N. Kholodenko日期:07/20/2021,浏览量:1222,Q&A: 0
    [Abstract]

    This protocol illustrates a pipeline for modeling the nonlinear behavior of intracellular signaling pathways. At fixed spatial points, nonlinear signaling dynamics are described by ordinary differential equations (ODEs). At constant parameters, these ODEs may have multiple attractors, such as multiple steady states or limit cycles. Standard

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    Ethanol-induced Sedative Behavior: An Assay to Investigate Increased Dopamine Signaling in Caenorhabditis elegans
    乙醇诱导的镇静行为:一项在秀丽隐杆线虫研究增加多巴胺信号
    作者:Anuradha Singh, Kavita Babu and Pratima Pandey日期:07/05/2021,浏览量:887,Q&A: 0
    [Abstract]

    Dopamine (DA) signaling affects locomotion, feeding, learning, and memory in C. elegans. Various assays have been developed to study the proteins involved in these behaviors; however, these assays show behavioral output only when there is a drastic change in DA levels. We designed an assay capable of observing behavioral output even with only

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    AIMTOR, a BRET Biosensor for Live Recording of mTOR Activity in Cell Populations and Single Cells
    一种用于实时记录细胞群和单个细胞中mTOR活性的BRET生物传感器AIMTOR
    作者:Nathalie Bouquier, Enora Moutin, Julie Perroy and Vincent Ollendorff日期:04/20/2021,浏览量:1570,Q&A: 0
    [Abstract]

    Mammalian target of rapamycin (mTOR) controls many crucial cellular functions, including protein synthesis, cell size, energy metabolism, lysosome and mitochondria biogenesis, and autophagy. Consequently, deregulation of mTOR signaling plays a role in numerous pathological conditions such as cancer, metabolic disorders and neurological diseases.

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    A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations
    量化人类和小鼠B细胞亚群中BCR介导的磷酸化的通用实验方法
    作者:Jasper Rip, Rudi W. Hendriks and Odilia B. J. Corneth日期:02/05/2021,浏览量:934,Q&A: 0
    [Abstract]

    Signal transduction is the process by which molecular signals are transmitted from the cell surface to its interior, resulting in functional changes inside the cell. B cell receptor (BCR) signaling is of crucial importance for B cells, as it regulates their differentiation, selection, survival, cellular activation and proliferation. Upon BCR

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    In vitro STING Activation with the cGAMP-STINGΔTM Signaling Complex
    cGAMP-STINGΔTM信号复合物体外激活STING
    作者:Yanpu He, Celestine Hong, Darrell J. Irvine, Jiahe Li and Paula T. Hammond日期:02/05/2021,浏览量:2376,Q&A: 0
    [Abstract] Activating the STING (stimulator of interferon genes) signaling pathway via administration of STING agonist cyclic GMP-AMP (cGAMP) has shown great promise in cancer immunotherapy. While state-of-the-art approaches have predominantly focused on the encapsulation of cGAMP into liposomes or polymersomes for cellular delivery, we discovered that the ...
    Immuno-electrophysiology on Neuromuscular Junctions of Drosophila Third Instar Larva
    果蝇三龄幼虫神经肌肉连接的免疫电生理研究
    作者:Raffaella Klima, Giulia Romano, Monsurat Gbadamosi, Aram Megighian and Fabian Feiguin日期:02/05/2021,浏览量:1019,Q&A: 0
    [Abstract]

    Alterations in synaptic transmission are critical early events in neuromuscular disorders. However, reliable methodologies to analyze the functional organization of the neuromuscular synapses are still needed. This manuscript provides a detailed protocol to analyze the molecular assembly of the neuromuscular synapses through

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    Quantification of the Surface Expression of G Protein-coupled Receptors Using Intact Live-cell Radioligand Binding Assays
    利用完整活细胞放射配体结合分析定量G蛋白偶联受体的表面表达
    作者:Xin Xu and Guangyu Wu日期:09/20/2020,浏览量:2300,Q&A: 0
    [Abstract] G protein-coupled receptors (GPCRs) are the most structurally diverse family of signaling proteins and regulate a variety of cell function. For most GPCRs, the cell surface is their functional destination where they are able to respond a wide range of extracellular stimuli, leading to the activation of intracellular signal transduction cascades. ...
    Production and Bioassay of a Diffusible Factor that Induces Gametophyte-to-Sporophyte Developmental Reprogramming in the Brown Alga Ectocarpus
    水云属褐藻配子体-孢子体发育重编程扩散因子的产生及生物测定
    [Abstract] The brown alga Ectocarpus has a haploid-diploid life cycle that involves alternation between two multicellular generations, the sporophyte and the gametophyte. Life cycle generation is not determined by ploidy but by a genetic system that includes two different three amino acid loop extension homeodomain transcription factors called ...
    Flow-cytometric Detection of Low-level Reactive Oxygen Species in Cell Lines and Primary Immune Cells
    细胞系和原代免疫细胞中的低水平活性氧的流式细胞仪检测
    作者:Kevin Bode, Corinna Link, Peter H. Krammer and Heiko Weyd日期:09/05/2020,浏览量:3093,Q&A: 0
    [Abstract] Depending on its concentration and cellular origin the production of reactive oxygen species (ROS) in the organism serves a variety of functions. While high concentrations during an oxidative burst are used to fight pathogens, low to moderate amounts of ROS act as signaling molecules important for several physiological processes such as regulation ...
    Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay
    使用IP-One评估Gαq/ 15信号:基于细胞高通量测定的单板转染和测定方案
    作者:Élie Besserer-Offroy, Rebecca L Brouillette, Jean-Michel Longpré and Philippe Sarret日期:08/20/2020,浏览量:2032,Q&A: 0
    [Abstract] Cell-based functional assays are an important part of compound screening and drug lead optimization, and they can also play a crucial role in the determination of the residues involved in ligand binding and signaling for a particular G-protein-coupled receptor. Conventional methods used for Gαq/15-coupled receptors rely on the use of ...