生物化学

分类

    现刊
    Cell-free Generation of COPII-coated Procollagen I Carriers
    COPII包被的前胶原I载体的无细胞生成
    作者:Lin Yuan, Satoshi Baba, Kanika Bajaj and Randy Schekman日期:11/20/2017,浏览量:5821,Q&A: 0
    [Abstract] The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This protocol describes the preparation of donor membrane and cytosol, the assembly of the reaction, and the ...
    Membrane Lipid Screen to Identify Molecular Targets of Biomolecules
    采用膜脂筛查法鉴定生物分子的分子靶点
    作者:John R. Jimah, Paul H. Schlesinger and Niraj H. Tolia日期:08/05/2017,浏览量:6676,Q&A: 0
    [Abstract] Proteins that bind to and disrupt cell membranes may target specific phospholipids. Here we describe a protocol to identify the lipid targets of proteins and biomolecules. First, we describe a screen to identify lipids in membranes that are specifically bound by the biomolecule of interest. Second, we describe a method for determining if the ...
    Assay to Measure Interactions between Purified Drp1 and Synthetic Liposomes
    量化检测纯化Drp1与合成脂质体之间的相互作用
    作者:Yoshihiro Adachi, Kie Itoh, Miho Iijima and Hiromi Sesaki日期:05/05/2017,浏览量:7974,Q&A: 0
    [Abstract] A mitochondrion is a dynamic intracellular organelle that actively divides and fuses to control its size, number and shape in cells. A regulated balance between mitochondrial division and fusion is fundamental to the function, distribution and turnover of mitochondria (Roy et al., 2015). Mitochondrial division is mediated by ...
    Liposome Flotation Assays for Phosphoinositide-protein Interaction
    磷酸肌醇-蛋白质相互作用的脂质体浮选分析法
    作者:Helene Tronchere and Frederic Boal日期:03/05/2017,浏览量:15497,Q&A: 0
    [Abstract] Phosphoinositides are rare membrane lipids involved in the control of the major cellular functions and signaling pathways. They are able to recruit specific effector proteins to the cytosolic face of plasma membrane and organelles to coordinate a vast variety of signaling and trafficking processes, as well to maintain specific identity of the ...
    Examination of the Interaction between a Membrane Active Peptide and Artificial Bilayers by Dual Polarisation Interferometry
    采用双偏振干涉法检测膜活性肽与人造双分子层之间的相互作用
    作者:Jennifer A.E. Payne, Tzong-Hsien Lee, Marilyn A. Anderson and Marie-Isabel Aguilar日期:01/05/2017,浏览量:5807,Q&A: 0
    [Abstract] Examining the interaction of peptides with lipid bilayers to determine binding kinetics is often performed using surface plasmon resonance (SPR). Here we describe the technique of dual polarisation interferometry (DPI) that provides not only information on the kinetics of the peptide binding to the bilayer, but also how the peptide affects the ...
    Determining Efficiency and Selectivity of Lipid Extraction by Perturbing Agents from Model Membranes
    采用源自模型膜的扰动剂测定脂质提取的效率和选择性
    作者:Michel Lafleur and Alexandre Therrien日期:11/20/2016,浏览量:6107,Q&A: 0
    [Abstract] Several membrane-perturbing agents extract lipids from membranes and, in some cases, this lipid efflux is lipid specific. In order to gain a better description of this phenomenon and to detail the intermolecular interactions that are involved, a method has been developed to characterize the extent and the specificity of membrane-lipid extraction ...
    Preparation of Chloroplast Lipid Membrane and Lipid-Protein Interaction Assay
    叶绿体膜脂的制备及脂质-蛋白质相互作用的分析
    作者:Kumiko Okazaki, Shin-ya Miyagishima and Hajime Wada日期:02/05/2016,浏览量:7676,Q&A: 0
    [Abstract] Lipid-Protein interaction assay is a method to search lipids, which are bound with proteins in vitro. Since membranes that are spotted with chloroplast lipids such as monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and sulfoquinovosyldiacylglycerol (SQDG) are not commercially available, we extracted these lipids ...
    In Gel Detection of Lipase Activity in Crude Plant Extracts (Olea europaea)
    植物粗提物(油橄榄)中脂肪酶活性的凝胶检测
    [Abstract] Here, we provide a detailed protocol describing a SDS-PAGE based procedure to assay in gel neutral lipase activity. Total protein extracts are separated by SDS-PAGE and gels are treated with lipase substrate-α-naphthyl palmitate. This long-chain fatty acid ester is hydrolysed by lipases present in the gel. The product resulting from this reaction ...
    Competitive ELISA for Protein-lipopolysaccharide (LPS) Binding
    竞争性酶联免疫吸附试验检测蛋白和脂多糖(LPS) 的结合
    作者:Victoria Martínez-Sernández and Florencio M. Ubeira日期:11/20/2014,浏览量:11760,Q&A: 1
    [Abstract] Lipopolysaccharide is the major constituent of the outer membrane of gram-negative bacteria and, once released from the bacterial surface into the bloodstream, is a potent activator of the host immune system, which can lead to septic shock. LPS has a hydrophilic region consisting of a repeating oligosaccharide that is strain-specific (O-antigen) ...
    Protein-lipid Interaction Analysis by Surface Plasmon Resonance (SPR)
    表面等离子共振 (SPR)法分析蛋白脂质的相互作用
    作者:Olga Lucia Baron and David Pauron日期:09/20/2014,浏览量:10924,Q&A: 0
    [Abstract] Interactions of lipids with proteins are essential events in the framework of biological membranes. Assessment of the affinity and specificity of protein-lipid binding can give useful information to elucidate cell membrane functions. Surface Plasmon Resonance (SPR) is a powerful technology to study macromolecular interactions, allowing direct and ...