微生物学

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    现刊
    Acid Hydrolysis for the Extraction of Archaeal Core Lipids and HPLC-MS Analysis
    酸水解法提取古生菌核心脂质及HPLC-MS分析
    作者:Maxime Tourte, Philippe Schaeffer, Vincent Grossi and Philippe M. Oger日期:08/20/2021,浏览量:475,Q&A: 0
    [Abstract]

    Lipid membranes are essential cellular elements as they provide cellular integrity and selective permeability under a broad range of environmental settings upon cell growth. In particular, Archaea are commonly recognized for their tolerance to extreme conditions, which is now widely accepted to stem from the unique structure of their lipids. While

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    Analysis of Gram-negative Bacteria Peptidoglycan by Ultra-performance Liquid Chromatography
    革兰阴性菌肽聚糖的超高效液相色谱法分析
    作者:Laura Alvarez, Baptiste Cordier, Sven van Teeffelen and Felipe Cava日期:10/05/2020,浏览量:2001,Q&A: 0
    [Abstract] Bacteria are surrounded by a protective peptidoglycan cell wall. Provided that this structure and the enzymes involved are the preferred target for our most successful antibiotics, determining its structural and chemical complexity is of the highest interest. Traditionally, high-performance liquid chromatography (HPLC) analyses have been ...
    Determination of the Cellular Ion Concentration in Saccharomyces cerevisiae Using ICP-AES
    ICP-AES法测定酿酒酵母细胞离子浓度
    作者:Louise Thines, Anne Iserentant and Pierre Morsomme日期:08/20/2020,浏览量:1409,Q&A: 0
    [Abstract] The yeast Saccharomyces cerevisiae has been perceived over decades as a highly valuable model organism for the investigation of ion homeostasis. Indeed, many of the genes and biological systems that function in yeast ion homeostasis are conserved throughout unicellular eukaryotes to humans. In this context, measurement of the yeast ...
    Quantification of Nitric Oxide and Reactive Oxygen Species in Leishmania-infected J774A.1 Macrophages as a Response to the in vitro Treatment with a Natural Product Compound
    感染利什曼原虫J774A.1的巨噬细胞响应体外天然化合物处理的一氧化氮和活性氧定量分析
    作者:Kalliopi Karampetsou, Olga S. Koutsoni and Eleni Dotsika日期:12/05/2019,浏览量:2220,Q&A: 0
    [Abstract] Leishmaniasis is a parasitic disease caused by the obligatory intracellular protozoa Leishmania spp. Current therapeutic options are limited and thus, drug discovery against leishmaniasis is very important. Nevertheless, there is a great difficulty to develop therapeutic strategies against the disease because the parasite deploys various ...
    Intracellular cAMP Measurements in Candida albicans Biofilms
    白念珠菌生物膜细胞内cAMP的测定
    作者:Liuliu Jiang, Shengyan Chen, Kairui Sun, Peng Zhou and Xin Wei日期:12/05/2019,浏览量:2195,Q&A: 0
    [Abstract] Candida albicans is the most common cause of fungal infections worldwide. Infection by C. albicans is closely associated with its ability to form a biofilm, closely packed communities of cells attached to the surfaces of human tissues and implanted devices, in or on the host. When tested for susceptibility to antifungals, such as ...
    Detection of D-glutamate Production from the Dual Function Enzyme, 4-amino-4-deoxychorismate Lyase/D-amino Acid Transaminase, in Mycobacterium smegmatis
    从垢分支杆菌中通过双功能酶,4-氨基-4-脱氧胆固醇裂解酶/D-氨基酸转氨酶、转氨酶产生的D-谷氨酸的检测
    作者:Helen K. Opel-Reading, Roman Mortuza and Kurt L. Krause日期:01/05/2019,浏览量:3867,Q&A: 0
    [Abstract] D-amino acid transaminase (D-AAT) is able to synthesize both D-glutamate and D-alanine, according to the following reaction: D-alanine + α-ketoglutarate ⇌ D-glutamate + pyruvate. These two D-amino acids are essential components of the peptidoglycan layer of bacteria. In our recently published work, MSMEG_5795 from Mycobacterium smegmatis ...
    Extraction and Analysis of Bacterial Teichoic Acids
    细菌磷壁酸的提取与分析
    作者:Kelvin Kho and Timothy C. Meredith日期:11/05/2018,浏览量:6211,Q&A: 0
    [Abstract] Teichoic acids (TA) are anionic polymers comprised of polyol phosphate repeat units that are abundant in the cell wall of Gram-positive bacteria. Both wall teichoic acid (WTA) and lipoteichoic acid (LTA) play important roles in regulating cell wall remodeling as well as conferring antibiotic resistance. To analyze TA, we describe a polyacrylamide ...
    Extraction and Quantification of Polyphosphate (polyP) from Gram-negative Bacteria
    从革兰氏阴性菌中提取和测定多聚磷酸盐
    作者:Jan-Ulrik Dahl, Lihan Xie and Ursula Jakob日期:09/20/2018,浏览量:4433,Q&A: 0
    [Abstract] Polyphosphate (polyP), a universally conserved biomolecule, is composed of up to 1,000 phosphate monomers linked via phosphoanhydride bonds. Reaching levels in bacteria that are in the high nmoles per mg protein range, polyP plays important roles in biofilm formation and colonization, general stress protection and virulence. Various protocols for ...
    Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining
    采用气相色谱法和尼罗红染色法测定富养罗尔斯通菌中聚羟基丁酸酯(PHB)的含量
    作者:Janina R. Juengert, Stephanie Bresan and Dieter Jendrossek日期:03/05/2018,浏览量:12249,Q&A: 0
    [Abstract] Ralstonia eutropha H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to analyze PHB granules by fluorescence ...
    Identification and Quantification of Secondary Metabolites by LC-MS from Plant-associated Pseudomonas aurantiaca and Pseudomonas chlororaphis
    采用LC-MS法鉴定和定量植物相关桔黄假单胞菌和绿针铜绿假单胞菌中次级代谢产物
    作者:Izzah Shahid, Muhammad Rizwan and Samina Mehnaz日期:01/20/2018,浏览量:9256,Q&A: 0
    [Abstract] Increased antibiotic resistance of plants and human pathogens and continuous use of chemical fertilizers has pushed microbiologists to explore new microbial sources as potential antagonists. In this study, eight strains of Pseudomonas aurantiaca and Pseudomonas chlororaphis, have been isolated from different plant sources and ...