癌症生物学

分类

    现刊
    In vivo Tumor Growth and Spontaneous Metastasis Assays Using A549 Lung Cancer Cells
    利用A549肺癌细胞进行肿瘤生长和自发性转移的体内检测
    作者:Lei Qi, Teresa Knifley, Dava W. Piecoro, Piotr Rychahou, Jianrong Wu, Kathleen L. O’Connor and Min Chen日期:04/05/2020,浏览量:3122,Q&A: 0
    [Abstract] Metastasis accounts for the majority of cancer related deaths. The genetically engineered mouse (GEM) models and cell line-based subcutaneous and orthotopic mouse xenografts have been developed to study the metastatic process. By using lung cancer cell line A549 as an example, we present a modified protocol to establish the cell line-based ...
    Protocol for Murine/Mouse Platelets Isolation and Their Reintroduction in vivo
    鼠/小鼠血小板分离及其在体内再导入实验方案
    作者:Jae Hong Im and Ruth J. Muschel日期:02/20/2017,浏览量:15490,Q&A: 1
    [Abstract] Platelets and coagulation have long been known to be essential for metastasis in experimental models. In order to study the interactions between tumor cells, platelets and endothelium, we have adapted methods used in coagulation research for the isolation of platelets and their reintroduction into mice. Anti-coagulated murine blood served as the ...
    Immunostaining Protocol: P-Smad2 (Xenograft and Mice)
    免疫染色法:P-Smad2(磷酸化-Smad2,异种移植和小鼠)
    [Abstract] Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF-beta responses when injected into nude mice as shown by lack of p-SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we ...