分子生物学

分类

    现刊
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:0,Q&A: 0

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass

    ...
    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用
    作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:0,Q&A: 0

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:Victoria Begley, Lola de Miguel-Jiménez and Sebastián Chávez日期:06/20/2021,浏览量:0,Q&A: 0

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this

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    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:355,Q&A: 0

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples

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    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:606,Q&A: 0

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules

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    Transcriptional Run-on: Measuring Nascent Transcription at Specific Genomic Sites in Yeast
    连缀转录:测定酵母特定基因组位点的新生转录
    作者:Victoria Begley, Lola de Miguel-Jiménez and Sebastián Chávez日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    DNA transcription by RNA polymerases has always interested the scientific community as it is one of the most important processes involved in genome expression. This has led scientists to come up with different protocols allowing analysis of this process in specific locations across the genome by quantitating the amount of RNA polymerases

    ...
    Peptide-mediated Targeting of Nanoparticles with Chemical Cargoes to Chloroplasts in Arabidopsis Plants
    多肽介导的化学物质纳米粒对拟南芥叶绿体的靶向作用
    作者:Israel Santana, Peiguang Hu, Su-Ji Jeon, Chris Castillo, Hann Tu and Juan Pablo Giraldo日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Plant nanobiotechnology is a flourishing field that uses nanomaterials to study and engineer plant function. Applications of nanotechnology in plants have great potential as tools for improving crop yield, tolerance to disease and environmental stress, agrochemical delivery of pesticides and fertilizers, and genetic modification and transformation

    ...
    Differential Analysis of N-glycopeptide Abundance and N-glycosylation Site Occupancy for Studying Protein N-glycosylation Dysregulation in Human Disease
    用于研究人类疾病中蛋白质N-糖基化失调的N-糖肽丰度和N-糖基化位点的差异分析
    作者:Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin日期:06/20/2021,浏览量:0,Q&A: 0
    [Abstract]

    Protein N-glycosylation plays a vital role in diverse cellular processes, and dysregulated N-glycosylation is implicated in a variety of human diseases including neurodegenerative disorders and cancer. With recent advances in high-resolution mass spectrometry-based glycoproteomics technologies enabling large-scale N-glycoproteome profiling of

    ...
    Mechanical Fractionation of Cultured Neuronal Cells into Cell Body and Neurite Fractions
    培养神经元细胞成细胞体和神经突部分的机械分离
    作者:Ankita Arora, Raeann Goering, Hei-Yong G. Lo and J. Matthew Taliaferro日期:06/05/2021,浏览量:606,Q&A: 0
    [Abstract]

    Many cells contain spatially defined subcellular regions that perform specialized tasks enabled by localized proteins. The subcellular distribution of these localized proteins is often facilitated by the subcellular localization of the RNA molecules that encode them. A key question in the study of this process of RNA localization is the

    ...
    Simplified Epigenome Profiling Using Antibody-tethered Tagmentation
    基于抗体系标记的简化表观基因组图谱
    作者:Steven Henikoff, Jorja G. Henikoff and Kami Ahmad日期:06/05/2021,浏览量:355,Q&A: 0
    [Abstract]

    We previously introduced Cleavage Under Targets & Tagmentation (CUT&Tag), an epigenomic profiling method in which antibody tethering of the Tn5 transposase to a chromatin epitope of interest maps specific chromatin features in small samples and single cells. With CUT&Tag, intact cells or nuclei are permeabilized, followed by successive

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    In vivo CD40 Silencing by siRNA Infusion in Rodents and Evaluation by Kidney Immunostaining
    小鼠体内siRNA转染CD40沉默和肾免疫染色评价
    [Abstract]

    The co-stimulatory molecule CD40 and its ligand CD40L play a key role in the regulation of immunological processes and are involved in the pathophysiology of autoimmune and inflammatory diseases. Inhibition of the CD40-CD40L axis is a promising therapy, and a number of strategies and techniques have been designed to hinder its functionality. Our

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    Surface Engineering and Multimodal Imaging of Multistage Delivery Vectors in Metastatic Breast Cancer
    转移性乳腺癌多期转运载体的表面工程和多模态成像
    作者:Shreya Goel, Mauro Ferrari and Haifa Shen日期:05/20/2021,浏览量:804,Q&A: 0
    [Abstract]

    The design of effective nanoformulations that target metastatic breast cancers is challenging due to a lack of competent imaging and image analysis protocols that can capture the interactions between the injected nanoparticles and metastatic lesions. Here, we describe the integration of in vivo whole-body PET-CT with high temporal resolution, ex

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    A New Method for Studying RNA-binding Proteins on Specific RNAs
    一种研究特异性RNA结合蛋白的新方法
    作者:Weiping Sun, Ziheng Zhang, Ji-Long Liu and Min Zhuang日期:05/20/2021,浏览量:1362,Q&A: 0
    [Abstract]

    Proximity-based protein labeling has been developed to identify protein-nucleic acid interactions. We have reported a novel method termed CRUIS (CRISPR-based RNA-United Interacting System), which captures RNA-protein interactions in living cells by combining the RNA-binding capacity of CRISPR/Cas13 and the proximity-tagging activity of PUP-IT.

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    Identification of R-loop-forming Sequences in Drosophila melanogaster Embryos and Tissue Culture Cells Using DRIP-seq
    DRIP-seq法鉴定黑腹果蝇胚胎和组织培养细胞R-loop形成序列
    作者:Célia Alecki and Nicole J. Francis日期:05/05/2021,浏览量:681,Q&A: 0
    [Abstract]

    R-loops are non-canonical nucleic structures composed of an RNA–DNA hybrid and a displaced ssDNA. Originally identified as a source of genomic instability, R-loops have been shown over the last decade to be involved in the targeting of proteins and to be associated with different histone modifications, suggesting a regulatory function. In

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    ATAC-Seq-based Identification of Extrachromosomal Circular DNA in Mammalian Cells and Its Validation Using Inverse PCR and FISH
    基于ATAC-Seq的哺乳动物细胞染色体外环状DNA鉴定及其反向PCR和FISH验证
    作者:Zhangli Su, Shekhar Saha, Teressa Paulsen, Pankaj Kumar and Anindya Dutta日期:05/05/2021,浏览量:755,Q&A: 0
    [Abstract]

    Recent studies from multiple labs including ours have demonstrated the importance of extrachromosomal circular DNA (eccDNA) from yeast to humans (Shibata et al., 2012; Dillon et al., 2015; Møller et al., 2016; Kumar et al., 2017; Turner et al., 2017; Kim et al., 2020). More recently, it has been found that cancer cells obtain a selective

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