分子生物学

分类

    现刊
    Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization
    基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位
    作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:279,Q&A: 0
    Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism ...
    Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry
    利用流式细胞术进行哺乳动物活细胞中Halo 标记蛋白细胞丰度测定
    作者:Claudia Cattoglio, Xavier Darzacq, Robert Tjian and Anders Sejr Hansen日期:02/20/2020,浏览量:365,Q&A: 0
    Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or ...
    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert Tjian and Anders S. Hansen日期:02/20/2020,浏览量:418,Q&A: 0
    Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein ...
    Surface Plasmon Resonance Analysis of the Protein-protein Binding Specificity Using Autolab ESPIRIT
    利用Autolab ESPIRIT进行表面等离子体共振分析蛋白间结合特异性
    作者:Pragyan Parimita Rath, Gaurav Anand and Shalini Agarwal日期:02/20/2020,浏览量:142,Q&A: 0
    Direct protein-protein interactions are known to regulate a wide range of cellular activities. To understand these contacts one can employ various experimental methods like Dynamic Light Scattering (DLS), Fluorescence Resonance Energy Transfer ...
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:85,Q&A: 0
    Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many ...
    RNA Sequencing of Single Myofibers from Mus musculus
    小鼠单肌纤维的RNA序列分析
    作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:304,Q&A: 0
    Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:121,Q&A: 0
    Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. ...
    Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization
    基于高分辨率显微镜的双分子荧光互补用于蛋白复合体装配和共定位
    作者:Jingjing Chen, Zulin Yu, Jay R. Unruh, Brian D. Slaughter and Sue L. Jaspersen日期:02/20/2020,浏览量:279,Q&A: 0
    [Abstract] Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes in vivo. While recent advances in super-resolution ...
    Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry
    利用流式细胞术进行哺乳动物活细胞中Halo 标记蛋白细胞丰度测定
    作者:Claudia Cattoglio, Xavier Darzacq, Robert Tjian and Anders Sejr Hansen日期:02/20/2020,浏览量:365,Q&A: 0
    [Abstract] Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or require sophisticated experimental and computational infrastructure (e.g., fluorescence ...
    Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation
    共免疫沉淀法评价哺乳动物核蛋白的自身相互作用
    作者:Claudia Cattoglio, Iryna Pustova, Xavier Darzacq, Robert Tjian and Anders S. Hansen日期:02/20/2020,浏览量:418,Q&A: 0
    [Abstract] Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that several nuclear proteins ...
    Surface Plasmon Resonance Analysis of the Protein-protein Binding Specificity Using Autolab ESPIRIT
    利用Autolab ESPIRIT进行表面等离子体共振分析蛋白间结合特异性
    作者:Pragyan Parimita Rath, Gaurav Anand and Shalini Agarwal日期:02/20/2020,浏览量:142,Q&A: 0
    [Abstract] Direct protein-protein interactions are known to regulate a wide range of cellular activities. To understand these contacts one can employ various experimental methods like Dynamic Light Scattering (DLS), Fluorescence Resonance Energy Transfer (FRET), Isothermal titration calorimetry (ITC), Chemical crosslinking, Co-immunoprecipitation (Co-IP), ...
    Construction of Antisense RNA-mediated Gene Knock-down Strains in the Cyanobacterium Anabaena sp. PCC 7120
    反义RNA介导的鱼腥藻PCC 7120基因敲除株的构建
    作者:Amit Srivastava, Anand Ballal, Karl Forchhammer and Anil Kumar Tripathi日期:02/20/2020,浏览量:85,Q&A: 0
    [Abstract] Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life function and hence cannot be deleted, causing a ...
    RNA Sequencing of Single Myofibers from Mus musculus
    小鼠单肌纤维的RNA序列分析
    作者:Darren M. Blackburn, Felicia Lazure and Vahab D. Soleimani日期:02/20/2020,浏览量:304,Q&A: 0
    [Abstract] Whole transcriptome analysis is a key method in biology that allows researchers to determine the effect a condition has on gene regulation. One difficulty in RNA sequencing of muscle is that traditional methods are performed on the whole muscle, but this captures non-myogenic cells that are part of the muscle. In order to analyze only the ...
    ChIP-Seq from Limited Starting Material of K562 Cells and Drosophila Neuroblasts Using Tagmentation Assisted Fragmentation Approach
    用Tagmentation Assisted Fragmentation法从K562细胞和果蝇神经母细胞的有限起始材料中提取芯片序列
    作者:Junaid Akhtar, Piyush More and Steffen Albrecht日期:02/20/2020,浏览量:121,Q&A: 0
    [Abstract] Chromatin immunoprecipitation is extensively used to investigate the epigenetic profile and transcription factor binding sites in the genome. However, when the starting material is limited, the conventional ChIP-Seq approach cannot be implemented. This protocol describes a method that can be used to generate the chromatin profiles from as low as ...
    Transcervical Mouse Infections with Chlamydia trachomatis and Determination of Bacterial Burden
    小鼠宫颈沙眼衣原体感染及细菌负荷测定
    作者:Karthika Rajeeve and Rajeeve Sivadasan日期:02/05/2020,浏览量:226,Q&A: 0
    [Abstract] Chlamydia trachomatis is an obligate human pathogen. It infects the genital tract of humans ascending into the fallopian tube, exacerbated by chronic pelvic pain, pelvic inflammatory disease, and fallopian tube scaring resulting in infertility and other malignancies. The major hurdle in controlling chlamydial spread is that the ...
    Structural Alignment and Covariation Analysis of RNA Sequences
    RNA序列的结构比对和协变分析
    作者:Nicolas J. Tourasse and Fabien Darfeuille日期:02/05/2020,浏览量:259,Q&A: 0
    [Abstract] RNA molecules adopt defined structural conformations that are essential to exert their function. During the course of evolution, the structure of a given RNA can be maintained via compensatory base-pair changes that occur among covarying nucleotides in paired regions. Therefore, for comparative, structural, and evolutionary studies of RNA ...
    Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISH
    利用显色原位杂交进行固定细胞和组织中单个RNA的检测
    作者:Meng Jiang, Chen Lin and Rongqin Ke日期:02/05/2020,浏览量:212,Q&A: 0
    [Abstract] Visualization of RNA molecules in situ helps to better understand the functions of expressed genes. Currently, most conventional in situ hybridization methods for visualization of individual RNAs are based on fluorescence detection. Herein we present a chromogenic in situ hybridization protocol for visualization of ...