分子生物学

分类

    现刊
    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:Stefan Bohn日期:09/20/2021,浏览量:375,Q&A: 0

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in

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    Construction of DNA/RNA Triplex Helices Based on GAA/TTC Trinucleotide Repeats
    基于GAA/TTC三核苷酸重复序列构建DNA/RNA三链螺旋
    作者:Jiahui Zhang, Ashkan Fakharzadeh, Feng Pan, Christopher Roland and Celeste Sagui日期:09/20/2021,浏览量:356,Q&A: 0

    Atypical DNA and RNA secondary structures play a crucial role in simple sequence repeat (SSR) diseases, which are associated with a class of neurological and neuromuscular disorders known as “anticipation diseases,” where the age of

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    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:Sébastien Darras日期:09/20/2021,浏览量:528,Q&A: 0

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for

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    Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing
    利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析
    作者:Yang Yang and Weixing Shan日期:09/20/2021,浏览量:265,Q&A: 0

    Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and

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    Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants
    农杆菌介导粳稻成熟胚和再生转基因植株的转化
    作者:Ammar Elakhdar, Masako Fukuda and Takahiko Kubo日期:09/20/2021,浏览量:342,Q&A: 0

    Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice.

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    Protocol for RNA-seq Expression Analysis in Yeast
    酵母RNA-seq表达分析方法
    作者:Stefan Bohn日期:09/20/2021,浏览量:375,Q&A: 0
    [Abstract]

    Genome-wide sequencing of RNA (RNA-seq) has become an inexpensive tool to gain key insights into cellular and disease mechanisms. Sample preparation and sequencing are streamlined and allow the acquisition of hundreds of gene expression profiles in a few days; however, in particular, data processing, curation, and analysis involve numerous steps

    ...
    Construction of DNA/RNA Triplex Helices Based on GAA/TTC Trinucleotide Repeats
    基于GAA/TTC三核苷酸重复序列构建DNA/RNA三链螺旋
    作者:Jiahui Zhang, Ashkan Fakharzadeh, Feng Pan, Christopher Roland and Celeste Sagui日期:09/20/2021,浏览量:356,Q&A: 0
    [Abstract]

    Atypical DNA and RNA secondary structures play a crucial role in simple sequence repeat (SSR) diseases, which are associated with a class of neurological and neuromuscular disorders known as “anticipation diseases,” where the age of disease onset decreases and the severity of the disease is increased as the intergenerational expansion

    ...
    En masse DNA Electroporation for in vivo Transcriptional Assay in Ascidian Embryos
    海鞘胚胎中DNA电穿孔的体内转录分析
    作者:Sébastien Darras日期:09/20/2021,浏览量:528,Q&A: 0
    [Abstract]

    Ascidian embryos are powerful models for functional genomics, in particular, due to the ease of generating a large number of transgenic embryos by electroporation. In addition, the small size of their genome makes them an attractive model for studying cis-regulatory elements that control gene expression during embryonic development. Here, I

    ...
    Quantitative Analysis of RNA Editing at Specific Sites in Plant Mitochondria or Chloroplasts Using DNA Sequencing
    利用DNA测序对植物线粒体或叶绿体特定位点RNA编辑的定量分析
    作者:Yang Yang and Weixing Shan日期:09/20/2021,浏览量:265,Q&A: 0
    [Abstract]

    Cytidine-to-uridine (C-to-U) RNA editing is one of the most important post-transcriptional RNA processing in plant mitochondria and chloroplasts. Several techniques have been developed to detect the RNA editing efficiency in plant mitochondria and chloroplasts, such as poisoned primer extension (PPE) assays, high-resolution melting (HRM) analysis,

    ...
    Agrobacterium-mediated Transformation of Japonica Rice Using Mature Embryos and Regenerated Transgenic Plants
    农杆菌介导粳稻成熟胚和再生转基因植株的转化
    作者:Ammar Elakhdar, Masako Fukuda and Takahiko Kubo日期:09/20/2021,浏览量:342,Q&A: 0
    [Abstract]

    Identification of novel genes and their functions in rice is a critical step to improve economic traits. Agrobacterium tumefaciens-mediated transformation is a proven method in many laboratories and widely adopted for genetic engineering in rice. However, the efficiency of gene transfer by Agrobacterium in rice is low, particularly among japonica

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    In situ Hybridization of miRNAs in Human Embryonic Kidney and Human Pluripotent Stem Cell-derived Kidney Organoids
    在人胚肾和人多能干细胞衍生的肾类器官中微小RNA的原位杂交
    作者:Filipa M. Lopes, Susan J. Kimber and Ioannis Bantounas日期:09/05/2021,浏览量:421,Q&A: 0
    [Abstract]

    MicroRNAs are small RNAs that negatively regulate gene expression and play an important role in fine-tuning molecular pathways during development. There is increasing interest in studying their function in the kidney, but the majority of studies to date use kidney cell lines and assess the total amounts of miRNAs of interest either by qPCR or by

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    Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (KD) and the Effective Concentration (EC50) of an Interplaying Effector Molecule Using Bio-layer Interferometry
    蛋白质-蛋白质复合物的动力学分析,以确定其电离常数 (KD) 和使用生物层干涉法相互作用的效应分子的有效浓度 (EC50)
    作者:Tim Orthwein, Luciano F. Huergo, Karl Forchhammer and Khaled A. Selim日期:09/05/2021,浏览量:1019,Q&A: 0
    [Abstract]

    Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the KD of a protein complex using a 6×His tagged fusion protein as bait immobilized on the NTA sensor chip of the

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    Direct-TRI: High-throughput RNA-extracting Method for all Stages of Zebrafish Development
    Direct-TRI: 斑马鱼发育各阶段高通量RNA提取方法
    作者:Kota Ujibe, Kanako Nishimura, Makoto Kashima and Hiromi Hirata日期:09/05/2021,浏览量:483,Q&A: 0
    [Abstract]

    Recent popularization of next-generation sequencing enables conducting easy transcriptome analysis. Nevertheless, substantial RNA isolation work prior to RNA sequencing, as well as the high cost involved, still makes the routine use of large-scale transcriptome analysis difficult. For example, conventional phenol-chloroform RNA extraction cannot

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    In vitro Cleavage and Electrophoretic Mobility Shift Assays for Very Fast CRISPR
    快速CRISPR的体外裂解和电泳迁移率位移测定
    作者:Roger S. Zou, Yang Liu and Taekjip Ha日期:09/05/2021,浏览量:735,Q&A: 0
    [Abstract]

    CRISPR-Cas9 has transformed biomedical research and medicine through convenient and targeted manipulation of DNA. Time- and spatially-resolved control over Cas9 activity through the recently developed very fast CRISPR (vfCRISPR) system have facilitated comprehensive studies of DNA damage and repair. Understanding the fundamental principles of Cas9

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    Measurement of LRRK2 Kinase Activity by Proximity Ligation Assay
    通过邻近连接测定法测量 LRRK2 激酶活性
    作者:Matthew T. Keeney, Eric K. Hoffman, J. Timothy Greenamyre and Roberto Di Maio日期:09/05/2021,浏览量:505,Q&A: 0
    [Abstract]

    Missense mutations in leucine rich-repeat kinase 2 (LRRK2) cause forms of familial Parkinson’s disease and have been linked to ‘idiopathic’ Parkinson’s disease. Assessment of LRRK2 kinase activity has been very challenging due to its size, complex structure, and relatively low abundance. A standard in the field to assess

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