分子生物学

分类

    现刊
    Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale
    源自生姜及根茎的生姜源性纳米颗粒(GDNPs)的分离、纯化和鉴定
    作者:Junsik Sung, Chunhua Yang, Emilie Viennois, Mingzhen Zhang and Didier Merlin日期:10/05/2019,浏览量:201,Q&A: 0
    Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels ...
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell ...
    Isolation and Transcriptomic Profiling of Single Myofibers from Mice
    小鼠单根肌纤维的分离和转录组分析
    Skeletal muscle is composed of different cells and myofiber types, with distinct metabolic and structural features. Generally, transcriptomic analysis of skeletal muscle is performed using whole muscle, resulting in average information as all cells ...
    ATAC-seq on Sorted Adult Mouse Neurons
    ATAC-seq用于分选成熟小鼠神经元
    作者:Erin A. Clark, Yasuyuki Shima and Sacha Nelson日期:10/05/2019,浏览量:186,Q&A: 0
    Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular and biochemical assays that probe the genome are critical tools in exploring mechanisms of transcription regulation ...
    Identification of Heteroreceptors Complexes and Signal Transduction Events Using Bioluminescence Resonance Energy Transfer (BRET)
    利用生物发光共振能量转移进行异受体复合物和信号转导的鉴定
    作者:Irene Reyes-Resina, Jasmina Jiménez, Gemma Navarro and Rafael Franco日期:10/05/2019,浏览量:142,Q&A: 0
    Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect ...
    Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
    酿酒酵母中非自身hsp70蛋白的功能分析
    作者:Laura E. Knighton, Lizbeth P. Saa, Adam M. Reitzel and Andrew W. Truman日期:10/05/2019,浏览量:99,Q&A: 0
    Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial ...
    In situ Hybridization of Plant-parasitic Nematode Globodera pallida Juveniles to Detect Gene Expression
    原位杂交检测马铃薯白胞囊线虫的基因表达
    作者:Joanna Kud, Nejra Solo, Allan Caplan, Joseph C. Kuhl, Louise-Marie Dandurand and Fangming Xiao日期:09/20/2019,浏览量:239,Q&A: 0
    In this study, we describe a standard whole mount in situ hybridization method which is used to determine the spatial-temporal expression pattern of genes from Globodera spp. Unlike more invasive radioactive labeling approaches, ...
    A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
    一种基于phoa stii技术的 大肠杆菌中高效分泌表达的fab片段纯化
    作者:Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu and Huili Lu日期:09/20/2019,浏览量:200,Q&A: 0
    In comparison with full-length IgGs, antigen binding fragments (Fabs) are smaller in size and do not require the complexed post-translational modification. Therefore, Fab can be cost-effectively produced using an Escherichia coli (E. coli ...
    Using indCAPS to Detect CRISPR/Cas9 Induced Mutations
    利用indCAPS检测CRISPR/Cas9诱导的突变
    作者:Charles Hodgens, Zachary L. Nimchuk and Joseph J. Kieber日期:09/20/2019,浏览量:183,Q&A: 0
    Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an amplified DNA fragment. A mutation that disrupts or ...
    Yeast Single-cell RNA-seq, Cell by Cell and Step by Step
    单细胞酵母逐个单步RNA-seq操作方法
    Single-cell RNA-seq (scRNA-seq) has become an established method for uncovering the intrinsic complexity within populations. Even within seemingly homogenous populations of isogenic yeast cells, there is a high degree of heterogeneity that ...
    A Refined Protocol for Identifying Citrulline-specific Monoclonal Antibodies from Single Human B Cells from Rheumatoid Arthritis Patient Material
    类风湿性关节炎患者单个B细胞中瓜氨酸特异单克隆抗体的改进鉴定方案
    We describe here a detailed, refined protocol for the generation of citrulline-specific monoclonal antibodies from single human B cells from rheumatoid arthritis (RA) patients. This protocol provides a detailed guide of the procedure starting from ...
    PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
    PEG介导的稳定的Cyanidioschizon merolae细胞核和叶绿体转化
    作者:Maksymilian Zienkiewicz, Tomasz Krupnik, Anna Drożak and Kinga Kania日期:09/05/2019,浏览量:244,Q&A: 0
    The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been ...
    A Widely Applicable Urea-based Fluorescent/Colorimetric mRNA in situ Hybridization Protocol
    一种广泛应用的尿素荧光/比色mrna原位杂交技术
    作者:Chiara Sinigaglia日期:09/05/2019,浏览量:726,Q&A: 0
    In situ hybridization methods are routinely employed to detect nucleic acid sequences, allowing to localize gene expression or to study chromosomal organization in their native context. These methods rely on the pairwise binding of a ...
    Development of an Efficient Transformation System for Halotolerant Yeast Debaryomyces hansenii CBS767
    耐盐汉斯德巴氏酵母菌CBS767的一种高效转化体系的建立
    作者:Anu P. Minhas and Dipanwita Biswas日期:09/05/2019,浏览量:224,Q&A: 0
    Debaryomyces hansenii is one of the most osmotolerant and halotolerant yeasts. Further, its association with traditional cheese and meat products imparting special flavors to these products project this yeast with enormous biotechnological ...
    A Novel Dual Lentiviral CRISPR-based Transcriptional Activation System for Gene Expression Regulation in Neurons
    一种新型基于CAS9双载体慢病毒转录激活系统的神经组织基因表达调控
    作者:Katherine E. Savell, Faraz A. Sultan and Jeremy J. Day日期:09/05/2019,浏览量:708,Q&A: 0
    Robust and efficient gene expression control enables the study of a gene’s function in the central nervous system. Advances in CRISPR-based technology provide new avenues not only for gene editing, but for complex transcriptional control. Here, we ...
    Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes
    一种改良的Smart-seq2样品制备方案在小鼠卵母细胞中应用稀有细胞全长单细胞mRNA测序
    作者:Rebecca S. Treger, Scott D. Pope, Xiaojun Xing and Akiko Iwasaki日期:08/20/2019,浏览量:439,Q&A: 0
    Endogenous retroviruses (ERV) are transposable retroelements that form ~10% of the murine genome and whose family members are differentially expressed throughout embryogenesis. However, precise regulation of ERV in germ cells remains unclear. To ...
    Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse Brain
    用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建
    作者:Hiroshi Yamaguchi and Luis de Lecea日期:08/20/2019,浏览量:576,Q&A: 0
    Recently developed gene editing technologies based on engineered CRISPR/Cas9 systems enables researchers to disrupt genes in a cell type-specific manner in the adult mouse brain. Using these technologies, we recently showed that the dopamine ...
    Measurement of the Length of the Integrated Donor DNA during Bacillus subtilis Natural Chromosomal Transformation
    枯草芽孢杆菌自然遗传转化过程中完整供体DNA长度的测定
    作者:Ester Serrano and Begoña Carrasco日期:08/20/2019,浏览量:335,Q&A: 0
    For natural transformation to occur, bacterial cells must first develop a programmed physiological state called competence. Competence in Bacillus subtilis, which occurs only in a fraction of cells, is a transient stress response that ...
    Three-dimensional Reconstruction and Quantification of Proteins and mRNAs at the Single-cell Level in Cultured Cells
    培养细胞中蛋白和mRNA在单细胞水平上的三维重构和量化分析
    作者:Xueer Jiang, Ingrid Brust-Mascher and Li-En Jao日期:08/20/2019,浏览量:649,Q&A: 0
    Gene expression is often regulated by the abundance, localization, and translation of mRNAs in both space and time. Being able to visualize mRNAs and protein products in single cells is critical to understand this regulatory process. The development ...
    A Radioactive in vitro ERK3 Kinase Assay
    体外ERK3激酶的放射性分析
    作者:Lobna Elkhadragy and Weiwen Long日期:08/20/2019,浏览量:361,Q&A: 0
    Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that have an important role in signal transduction. Extracellular signal-regulated kinase 3 (ERK3), also known as MAPK6, is an atypical MAPK. Here, we describe in detail an in ...
    Genotyping of the OATP1B1 c. 521 T>C Polymorphism from the Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens: An Optimized Protocol
    对FFPE组织样品中OATP1B1 c. 521 T>C多态性进行基因分型的优化实验方法
    作者:Alexandra R Crowe, Jonathan Miller and Wei Yue日期:08/20/2019,浏览量:240,Q&A: 0
    Organic anion transporting polypeptide (OATP) 1B1 is a liver-specific transport protein that plays an important role in hepatic drug disposition. It transports many drugs from the blood into the liver, including lipid-lowering statins. The c. 521 ...
    Preparation of RNA 3’ End Sequencing Libraries of Total and 4-thiouracil Labeled RNA for Simultaneous Measurement of Transcription, RNA Synthesis and Decay in S. cerevisiae
    制备酿酒酵母总RNA和4-硫尿RNA的RNA3'端测序文库用于转录,RNA合成和降解的同步测定
    作者:Manfred Schmid, Agnieszka Tudek and Torben Heick Jensen日期:03/20/2019,浏览量:1217,Q&A: 0
    Cellular RNA levels are determined by the rates of RNA transcription from the gene template and subsequent RNA stability. Knowledge about both transcription and RNA decay is, therefore, necessary to interpret RNA levels and gene expression, ...
    Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
    病毒染色体构象俘获技术用于裂解病毒和细胞基因组之间反向相互作用位点的检测
    作者:Kinjal Majumder, Maria Boftsi and David J Pintel日期:03/20/2019,浏览量:1048,Q&A: 0
    The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding ...
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    整体原位杂交检测mRNA并提取DNA检测斑马鱼胚胎的基因分型
    作者:Rachna Narayanan and Andrew C. Oates日期:03/20/2019,浏览量:2520,Q&A: 0
    In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes ...
    Biofilm Assays on Fibrinogen-coated Silicone Catheters and 96-well Polystyrene Plates
    在纤维蛋白原包覆的硅胶导管和96孔聚苯乙烯板上进行生物膜检测
    作者:Cristina Colomer-Winter, José A. Lemos and Ana L. Flores-Mireles日期:03/20/2019,浏览量:1330,Q&A: 0
    Biofilm formation is a well-known bacterial strategy that protects cells from hostile environments. During infection, bacteria found in a biofilm community are less sensitive to antibiotics and to the immune response, often allowing them to colonize ...
    Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
    利用聚乙二醇凝胶和Northern印记法定量分析来自人细胞的tRNA 的Q核苷修饰水平
    作者:Zaneta Matuszek and Tao Pan日期:03/20/2019,浏览量:1097,Q&A: 0
    Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at ...
    Isolation, Purification, and Characterization of Ginger-derived Nanoparticles (GDNPs) from Ginger, Rhizome of Zingiber officinale
    源自生姜及根茎的生姜源性纳米颗粒(GDNPs)的分离、纯化和鉴定
    作者:Junsik Sung, Chunhua Yang, Emilie Viennois, Mingzhen Zhang and Didier Merlin日期:10/05/2019,浏览量:201,Q&A: 0
    [Abstract] Factors implicated in the pathophysiology of intestinal inflammation include defects in intestinal epithelial barrier function, abnormal immune responses, and activities of the gut microbiota. Current agents used to treat human Inflammatory Bowels Disease (IBD), chronic inflammation of digestive tract, have serious side effects. In addition, most ...
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    [Abstract] Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases ...
    Isolation and Transcriptomic Profiling of Single Myofibers from Mice
    小鼠单根肌纤维的分离和转录组分析
    [Abstract] Skeletal muscle is composed of different cells and myofiber types, with distinct metabolic and structural features. Generally, transcriptomic analysis of skeletal muscle is performed using whole muscle, resulting in average information as all cells composing the organ contribute to the expression value detected for each gene with the loss of ...
    ATAC-seq on Sorted Adult Mouse Neurons
    ATAC-seq用于分选成熟小鼠神经元
    作者:Erin A. Clark, Yasuyuki Shima and Sacha Nelson日期:10/05/2019,浏览量:186,Q&A: 0
    [Abstract] Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular and biochemical assays that probe the genome are critical tools in exploring mechanisms of transcription regulation and cell type identity. The mammalian brain is composed of a huge diversity of cell types with ...
    Identification of Heteroreceptors Complexes and Signal Transduction Events Using Bioluminescence Resonance Energy Transfer (BRET)
    利用生物发光共振能量转移进行异受体复合物和信号转导的鉴定
    作者:Irene Reyes-Resina, Jasmina Jiménez, Gemma Navarro and Rafael Franco日期:10/05/2019,浏览量:142,Q&A: 0
    [Abstract] Detecting protein-protein interactions by co-immunoprecipitation provided a major advancement in the immunology research field. In the G-protein-coupled receptors (GPCRs) research field, colocalization and co-immunoprecipitation were used to detect interactions, but doubts arose due to specificity of the antibodies (monoclonal in the case of ...
    Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
    酿酒酵母中非自身hsp70蛋白的功能分析
    作者:Laura E. Knighton, Lizbeth P. Saa, Adam M. Reitzel and Andrew W. Truman日期:10/05/2019,浏览量:99,Q&A: 0
    [Abstract] Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial step, Hsp70 variants (such as Ssa1 point mutants and non-yeast versions such as Nematostella ...
    In situ Hybridization of Plant-parasitic Nematode Globodera pallida Juveniles to Detect Gene Expression
    原位杂交检测马铃薯白胞囊线虫的基因表达
    作者:Joanna Kud, Nejra Solo, Allan Caplan, Joseph C. Kuhl, Louise-Marie Dandurand and Fangming Xiao日期:09/20/2019,浏览量:239,Q&A: 0
    [Abstract] In this study, we describe a standard whole mount in situ hybridization method which is used to determine the spatial-temporal expression pattern of genes from Globodera spp. Unlike more invasive radioactive labeling approaches, this technique is based on a safe, highly specific enzyme-linked immunoassay where a Digoxigenin ...
    A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
    一种基于phoa stii技术的 大肠杆菌中高效分泌表达的fab片段纯化
    作者:Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu and Huili Lu日期:09/20/2019,浏览量:200,Q&A: 0
    [Abstract] In comparison with full-length IgGs, antigen binding fragments (Fabs) are smaller in size and do not require the complexed post-translational modification. Therefore, Fab can be cost-effectively produced using an Escherichia coli (E. coli) expression system. However, the disulfide-bonds containing exogenous protein, including ...
    Using indCAPS to Detect CRISPR/Cas9 Induced Mutations
    利用indCAPS检测CRISPR/Cas9诱导的突变
    作者:Charles Hodgens, Zachary L. Nimchuk and Joseph J. Kieber日期:09/20/2019,浏览量:183,Q&A: 0
    [Abstract] Cleaved amplified polymorphic sequences (CAPS) assays are useful tools for detecting small mutations such as single nucleotide polymorphisms (SNPs) or insertion/deletions (indels) present in an amplified DNA fragment. A mutation that disrupts or creates a restriction site will prevent cleavage by a restriction enzyme, allowing discrimination of ...
    Yeast Single-cell RNA-seq, Cell by Cell and Step by Step
    单细胞酵母逐个单步RNA-seq操作方法
    [Abstract] Single-cell RNA-seq (scRNA-seq) has become an established method for uncovering the intrinsic complexity within populations. Even within seemingly homogenous populations of isogenic yeast cells, there is a high degree of heterogeneity that originates from a compact and pervasively transcribed genome. Research with microorganisms such as yeast ...